NAT10 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA189183A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate(20µg), A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), NIH/3T3 whole cell lysate(20µg), HEK293 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), COLO205 whole cell lysate(20µg),Jurkat whole cell lysate(20µg),HepG2 whole cell lysate(20µg) All lanes: NAT10 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 116 kDa Observed band size: 130 kDa Exposure time：20s Overlay Peak curve showing jurkat cells stained with CSB-RA189183A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9H0A0

Target Names

NAT10

Alternative Names

ALP antibody; DKFZp434C116 antibody; FLJ10774 antibody; FLJ12179 antibody; FLJ23850 antibody; hALP antibody; KIAA1709 antibody; N acetyltransferase 10 antibody; N acetyltransferase 10 GCN5 related antibody; N acetyltransferase like antibody; N acetyltransferase like protein antibody; N-acetyltransferase 10 antibody; NAT10 antibody; NAT10_HUMAN antibody; NET43 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human NAT10

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NAT10, or N-acetyltransferase 10, is a multifunctional enzyme that plays critical roles in ribosome biogenesis, telomerase regulation, and RNA acetylation. As the only known enzyme capable of catalyzing ac4C modification on both RNA and DNA, NAT10 has emerged as a significant focus in cancer biology, cellular senescence research, and epitranscriptomics. Its involvement in cell proliferation and potential as a therapeutic target makes reliable detection tools essential for advancing these research areas.

This recombinant monoclonal antibody, clone 9C11, offers the consistency and reproducibility that demanding experiments require. Generated against a synthetic peptide derived from human NAT10 and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation. The rabbit IgG format, purified by affinity chromatography, ensures high purity for sensitive downstream applications.

Validation studies demonstrate robust performance across multiple experimental platforms. In western blot applications, this antibody successfully detects NAT10 across an extensive panel of human cell lines including SH-SY5Y, A549, HeLa, HEK293, PC-3, COLO205, Jurkat, and HepG2, as well as mouse NIH/3T3 cells, confirming cross-species reactivity. The observed band at approximately 130 kDa, slightly higher than the predicted 116 kDa molecular weight, likely reflects post-translational modifications such as glycosylation or phosphorylation characteristic of this nuclear protein. Flow cytometry validation in Jurkat cells demonstrates clear positive signal separation from isotype controls, supporting intracellular detection workflows.

Whether investigating RNA modification pathways, exploring NAT10's role in tumorigenesis, or studying ribosome assembly mechanisms, this antibody provides the reliability researchers need for reproducible results across western blot, flow cytometry, and ELISA applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA. May also catalyze the formation of ac4C at position 1337 in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. The relevance of the protein lysine acetyltransferase activity is however unsure in vivo. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Involved in the regulation of centrosome duplication by acetylating CENATAC during mitosis, promoting SASS6 proteasome degradation.

Gene References into Functions

immunohistochemistry staining of NAT10 in hepatocellular carcinoma tissues demonstrates that the cytoplasmic NAT10 is correlated with poorer prognosis compared with nuclear NAT10, while the membranous NAT10 predicts the poorest clinical outcome of the patients. PMID: 29634924
Increased NAT10 expression levels are associated with shortened hepatocellular carcinoma (HCC) patient survival and correlated with mutant p53 levels. NAT10 upregulates mutant p53 level and might enhance its tumorigenic activity. Hence, it is proposed that NAT10 could be a potential prognostic and therapeutic candidate for p53-mutated HCC. PMID: 28859621
NAT10 is acetylated in vivo and autoacetylated in vitro PMID: 27993683
NAT10 translocates to nucleoplasm to bind and acetylate p53 at K120 upon cellular stress. PMID: 26882543
The subcellular redistribution of NAT10 can be induced by decreases in GSK-3b activity. This redistribution increases cancer cell motility, and is, thus, correlated with invasive potential and poorer clinical outcome PMID: 24982245
Identification of NAT10 as responsible for both 18S rRNA and leucine/serine tRNA acetylation. PMID: 25653167
NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N(4)-acetylcytidine (ac(4)C) at position 1842 in the terminal helix of mammalian 18 S rRNA. PMID: 25411247
we identified hALP, a histone acetyl-transferase as a novel t-UTP PMID: 21177859
hALP is a nucleolar protein, and the nucleolar localization is mediated by its carboxy terminal domain. PMID: 18677378
The investigation suggested that hALP influences the activity of histone acetylation and could up-regulate telomerase activity through transactivation of hTERT promoter. PMID: 14592445
The above results suggested that NAT10 could be involved in DNA damage response and increased cellular resistance to genotoxicity. PMID: 17180247
chromosome de-condensation needs the function of an inner nuclear membrane (INM) protein hsSUN1 and a membrane-associated histone acetyltransferase (HAT), hALP. PMID: 17631499
NAT10 may play an important role in cell division through facilitating reformation of the nucleolus and midbody in the late phase of cell mitosis, and stabilization of microtubules. PMID: 19303003

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Subcellular Location

Nucleus, nucleolus. Midbody.

Protein Families

RNA cytidine acetyltransferase family, NAT10 subfamily

Database Links

HGNC: 29830

OMIM: 609221

KEGG: hsa:55226

STRING: 9606.ENSP00000257829

UniGene: Hs.577281

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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