NCS1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA944972A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: NCS1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time：3min IHC image of CSB-RA944972A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of hela cell with CSB-RA944972A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P62166

Target Names

NCS1

Alternative Names

Neuronal calcium sensor 1 (NCS-1) (Frequenin homolog) (Frequenin-like protein) (Frequenin-like ubiquitous protein), NCS1, FLUP FREQ

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from Human NCS1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9H5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Gene References into Functions

NCS-1, a calcium-binding protein, is associated with clinicopathologic features of aggressiveness in breast cancer cells and worse outcome in two breast cancer patient cohorts PMID: 28275088
Results show that although NCS-1 Var2mRNA can be detected, protein expression was not detectable. When overexpressed in cell lines NCS-1 Var2 appears to be folded properly, yet it has a lower calcium affinity. Because most NCS-1 binding to its protein partners requires calcium binding, it is unlikely that NCS-1 Var2 would play a major regulatory role in cells. PMID: 27575489
structural and dynamical response of NCS-1 protein to elevated temperature may be one of its intrinsic functional properties PMID: 27007011
Lithium normalizes gamma band oscillations mediated by P/Q-type calcium channels modulated by NCS-1. PMID: 27033453
Study determined the salient features of the folding free-energy landscapes of the Mg2+-bound and apo states of NCS-1 PMID: 26153708
results reveal the important role of salt bridges on the structural properties of NCS-1 protein and that R102Q mutation disables the dynamic relocation of C-terminus, which may block the binding of NCS-1 protein to its receptors PMID: 25343687
single-molecule optical tweezers were used to monitor misfolding reactions of the human neuronal calcium sensor-1. PMID: 25157171
A strong liason was characterized between myristoylation and cryptic EF-1 in NCS-1. PMID: 25565019
The functional link in substantia nigra dopamine neurons between Cav1.3- L-type-Ca2+ channels and D2-autoreceptor activity is an adaptive signalling network controlled by NCS-1. PMID: 24934288
NCS1 has evolved a remarkable complex interdomain cooperativity and a fundamentally different folding mechanism compared to structurally related proteins. PMID: 24012477
data suggest that genetic variants in the NCS-1 gene contribute to susceptibility of Cocaine Abuse in individuals of African descent. PMID: 22999924
[review] Receptor interaction properties in schizophrenia are dependent upon NCS-1, a protein found to regulate the phosphorylation, trafficking, and signaling profile of the D2 dopamine receptor (D2R) in neurons. PMID: 21777187
the C-terminal tail is important for regulating the conformational stability of NCS1 in Ca(2+)-activated state PMID: 22227393
analysis of the interaction between the D2 dopamine receptor and neuronal calcium sensor-1 PMID: 21875085
it is possible to protect cells from paclitaxel-induced degradation of NCS-1 by inhibiting calpain activity PMID: 21808066
NCS-1 is a novel regulator of cardiac calcium signaling, specifically in immature and hypertrophic hearts. PMID: 21737792
Impairment of the normal cycling of NCS-1 by the R102Q mutation could have subtle effects on neuronal signalling and physiology in the developing and adult brain. PMID: 20479890
up-regulated in the prefrontal cortex of schizophrenic and bipolar patients PMID: 12496348
Analysis with chimeric proteins between KChIP2 and NCS-1 reveals that the three regions of KChIP2 are necessary and sufficient for its effective binding to Kv4.3 protein PMID: 12928444
may serve as regulator of certain isoforms of phosphatidylinositol 4-kinase PMID: 14512421
key role for residues within the motif EELTRK in NCS-1 in keeping the myristoyl group exposed and allowing the protein to be constitutively membrane-associated. PMID: 14726528
NCS-1 is shown to be mainly associated with azurophilic granules in human neutrophils and promyelocitic cells and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules PMID: 14760944
These results demonstrate a novel role for NCS-1 and PI4Kbeta in regulating ERK1/2 signaling and inflammatory reactions in mast cells. PMID: 16837555
NCS-1 expression was diminished in CD4+T lymphocytes, CD19+ B lymphocytes and CD14+ monocytes of bipolar disorder patients and also decreased in CD4+ T lymphocytes and CD56+ natural killer cells of schizophrenia patients PMID: 19091302
occurrence of an unusual TG 3' splice site in intron 2 PMID: 17672918

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Subcellular Location

Golgi apparatus. Cell junction, synapse, postsynaptic density. Cytoplasm, perinuclear region. Cytoplasm. Cell membrane; Peripheral membrane protein. Membrane; Lipid-anchor.

Protein Families

Recoverin family

Database Links

HGNC: 3953

OMIM: 603315

KEGG: hsa:23413

STRING: 9606.ENSP00000361475

UniGene: Hs.642946

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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