NDUFB9 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA568204A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), K562 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HeLa whole cell lysate(30µg), A549 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Rat stomach tissue lysate(30µg) All lanes: NDUFB9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1min IHC image of CSB-RA568204A0HU diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA568204A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9Y6M9

Target Names

NDUFB9

Alternative Names

B22 antibody; CI B22 antibody; CI-B22 antibody; complex I B22 subunit antibody; Complex I-B22 antibody; DKFZp566O173 antibody; FLJ22885 antibody; I B22 antibody; LYR motif containing protein 3 antibody; LYR motif-containing protein 3 antibody; LYRM3 antibody; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody; NADH ubiquinone oxidoreductase B22 subunit antibody; NADH-ubiquinone oxidoreductase B22 subunit antibody; NDUB9_HUMAN antibody; Ndufb9 antibody; UQOR22 antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human NDUFB9

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4D7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Gene References into Functions

Down-Regulation of NDUFB9 Promotes Breast Cancer Cell Proliferation, Metastasis by Mediating Mitochondrial Metabolism. PMID: 26641458
Mutant NDUFB9 is a new cause of complex I deficiency. PMID: 22200994

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Protein Families

Complex I LYR family

Database Links

HGNC: 7704

OMIM: 601445

KEGG: hsa:4715

STRING: 9606.ENSP00000276689

UniGene: Hs.15977

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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