NFKBIB Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA933059A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Raji whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: IkB beta antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 38 kDa Observed band size: 48 kDa Exposure time：1min IHC image of CSB-RA933059A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA933059A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q15653

Target Names

NFKBIB

Alternative Names

NF-kappa-B inhibitor beta (NF-kappa-BIB) (I-kappa-B-beta) (IkB-B) (IkB-beta) (IkappaBbeta) (Thyroid receptor-interacting protein 9) (TR-interacting protein 9) (TRIP-9), NFKBIB, IKBB TRIP9

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human NFKBIB protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12B9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NFKBIB, also known as IκB-beta, serves as a critical regulator of the NF-κB signaling pathway by sequestering NF-κB transcription factors in the cytoplasm and preventing their nuclear translocation. This inhibitory function makes NFKBIB essential for controlling inflammatory responses, immune cell activation, and cell survival decisions. Researchers investigating NF-κB-dependent gene expression, inflammatory disease mechanisms, or immune signaling dynamics rely on precise detection of this key regulatory protein.

This recombinant monoclonal antibody, generated against a synthetic peptide from human NFKBIB, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced from a single rabbit clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations, ensuring your results remain comparable across experiments.

Validation across multiple applications demonstrates genuine workflow flexibility. Western blot analysis confirms robust detection in diverse human cell lines including Jurkat, HeLa, K562, HepG2, MCF-7, and Raji cells, as well as mouse NIH/3T3 cells, supporting cross-species reactivity for both human and mouse samples. The observed band at 48 kDa, higher than the predicted 38 kDa molecular weight, likely reflects post-translational modifications such as phosphorylation or glycosylation that are characteristic of IκB family proteins under physiological conditions. Immunohistochemistry validation in human testis tissue demonstrates suitability for spatial expression studies in fixed specimens, while flow cytometry analysis in Jurkat cells confirms utility for single-cell protein quantification.

This antibody supports investigations into NF-κB pathway regulation, inflammatory signaling, cancer biology, and immune cell function where reliable NFKBIB detection is fundamental to experimental success.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Gene References into Functions

The subcellular distributions of IkappaB and NFkappaB are indicative of carcinogenesis. Inhibition of XPO1 results in intranuclear retention of IkappaB, which inhibits NFkappaB and thereby provides a novel mechanism for drug therapy in sarcoma. This effect can be further enhanced in relatively selinexor-resistant sarcoma cell lines by pretreatment with the proteasome inhibitor carfilzomib. PMID: 28314790
data suggest that miRNA-4776 modulates Influenza A virus production in infected cells through NFKBIB expression, possibly through the modulation of NF-kappaB. PMID: 28448456
our data establish the importance of a novel tumor suppressive IKBB gene in abrogating angiogenesis in NPC via the NF-kappaB signalling pathway. PMID: 26227166
NFKBIBrs3136641TT single nucleotide polymorphism was associated with a significantly decreased risk of developing wheezing. PMID: 25326706
IkappaBbeta may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-kappaB/IkappaBbeta in spermatogenesis PMID: 12475944
VEGF increased Mn-superoxide dismutase promoter activity, an effect that was dependent on a second intronic NF-kappaB consensus motif. PMID: 15308628
data indicate that inhibition of NFkappa-B activity by the hepatitis C virus core protein might be related to its physical interaction with and interrupted nuclear localization of IKKbeta PMID: 15919917
None of the NFKBIB SNPs are associated with pneumococcal susceptibility. PMID: 17463416
increased I-kappaBbeta expression reversed NF-kappaB activation in cancer cells, compensating for the loss of I-kappaBalpha via TGase 2 polymerization. PMID: 18950638

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

NF-kappa-B inhibitor family

Tissue Specificity

Expressed in all tissues examined.

Database Links

HGNC: 7798

OMIM: 604495

KEGG: hsa:4793

STRING: 9606.ENSP00000312988

UniGene: Hs.9731

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Email: [email protected]
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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Chemokine signaling pathway B cell receptor signaling pathway NOD-like receptor signaling pathway T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation Adipocytokine signaling pathway Neurotrophin signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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