NME2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA564743A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HeLa whole cell lysate(30µg), A431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Mouse kidney tissue lysate(30µg) All lanes: NDKB antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time：5s Immunofluorescence staining of MCF-7 cell with CSB-RA564743A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA564743A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P22392

Target Names

NME2

Alternative Names

Nucleoside diphosphate kinase B (NDK B) (NDP kinase B) (EC 2.7.4.6) (C-myc purine-binding transcription factor PUF) (Histidine protein kinase NDKB) (EC 2.7.13.3) (nm23-H2), NME2, NM23B

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human NME2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NME2, also known as nucleoside diphosphate kinase B, plays a multifaceted role in cellular biology that extends well beyond its canonical enzymatic function. This protein participates in nucleotide metabolism, DNA binding, and transcriptional regulation, notably acting as a purine-binding transcription factor that influences c-myc expression. Its involvement in metastasis suppression and cellular differentiation makes NME2 a compelling target for cancer biology research and studies investigating gene regulation mechanisms.

This recombinant monoclonal antibody, clone 14G12, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced through controlled expression systems, you can expect consistent performance across experiments and between lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple experimental platforms. Western blot analysis detects a clean 17 kDa band matching the predicted molecular weight, confirmed across an extensive panel of human cell lines including A549, HepG2, HeLa, A431, HEK293, PC-3, MCF-7, and Jurkat cells, as well as mouse kidney tissue. This cross-species reactivity between human and mouse samples provides flexibility for researchers working with different model systems. Immunofluorescence staining in MCF-7 cells reveals clear cellular localization patterns, while flow cytometry validation in the same cell line shows distinct population separation from isotype controls, confirming utility for quantitative single-cell analyses.

The antibody's compatibility with ELISA, Western blot, immunofluorescence, and flow cytometry workflows makes it suitable for researchers investigating NME2's roles in tumor progression, metastatic potential, nucleotide metabolism, and transcriptional regulation across diverse experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not. Exhibits histidine protein kinase activity.

Gene References into Functions

Data suggest that NME2 participates in epigenetic repression of TERT expression via a mechanism that is dependent on interactions with G-quadruplex DNA; NME2 appears to interact with TERT promoter as NME2/REST/LSD1 complex. (NME2 = metastasis suppressor protein 2; TERT = telomerase reverse transcriptase; REST = RE1-silencing transcription factor; LSD1 = lysine specific demethylase 1) PMID: 28717007
Role of Interaction and Nucleoside Diphosphate Kinase B in Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator Function by cAMP-Dependent Protein Kinase A PMID: 26950439
NME2 may have an inhibitory activity on the propagation and invasion of gastric cancer cells. PMID: 25700270
Regulatory functions of Nm23-H2 in tumorigenesis: insights from biochemical to clinical perspectives PMID: 25413836
Recent findings demonstrate NME2 presence at telomere ends, association with telomerase, and NME2's role in inhibition of telomerase activity in cancer cells PMID: 25547372
A novel mechanism of CARMA3 in lung cancer stemness and metastasis through the negative regulation of NME2 was identified. PMID: 25906011
NDPK-B and NDPK-D were shown to bind efficiently to liposomes mimicking plasma membrane and mitochondrial inner membrane PMID: 25010650
Patients whose MPM tissues expressed elevated mRNA levels of BIRC5, DSP, NME2, and THBS2 showed a statistically significant shorter overall survival. PMID: 25771974
The results demonstrate that reduced NME2 levels lead to transcriptional de-repression of vinculin and regulate lung cancer metastasis. PMID: 25249619
nucleoside diphosphate kinase B (NDPK-B), activates TRPV5 channel activity and Ca(2+) flux, and this activation requires histidine 711 in the carboxy-terminal tail of TRPV5. PMID: 24523290
NDPKB is required for VEGF-induced angiogenesis and contributes to the correct localization of VEGF receptor type 2 and VE-cadherin at the endothelial adherens junctions. PMID: 25147336
PuF may regulate the APP gene promoter PMID: 23368879
post-transcriptional up-regulation of the tumor antigen NME2 is a common and specific property of CML closely associated with Bcr-Abl activity PMID: 22251158
NM23-H2 expression enhances tumorigenicity. PMID: 22192927
further demonstrate that shNDPK-B is released into the circulation in immunocompromized mice carrying the human breast carcinoma cell MDA-MB- PMID: 20830314
Observational study of gene-disease association, gene-gene interaction, and genetic testing. (HuGE Navigator) PMID: 20864512
NM23-H2 protein expression in human leukemia cell lines is related to cellular lineage and differentiation stages. PMID: 12007505
The nucleotide-binding site of NM23-H2 plays a critical role in not only the NDP kinase reaction but also the reactions involving covalent DNA binding and DNA cleavage catalysis. PMID: 12009894
Nm23-H2 had a cytoplasmic and nuclear localization but was induced to translocate to the plasma membrane upon stimulation of thromboxane A2 receptor beta to show extensive co-localization with the receptor. PMID: 14976202
novel association of NM23-H2 with ERbeta and show for the first time its expression in vascular cells and demonstrate regulation of its expression and localization by estrogen PMID: 17272673
Thus, these results indicate the existence of a previously undiscovered mechanism by which NM23-H2 involves in the regulation of Diva-mediated apoptosis. PMID: 17532299
NM23-H2 as an estrogen receptor beta-associated protein may play an important role in modulating the response to endogenous and exogenous estrogens. PMID: 17964137
describe the identification of NM23-H2 as a novel HLA-A32-restricted TAA of CML cells and demonstrate the presence of specifically reactive T cells in a patient 5 years after transplantation PMID: 18496563
While hPLIC proteins are not ubiquitinated by HERC3, HERC5 plays an important role in ubiquitination of Nm23B. PMID: 18535780
data indicate that NM23H2 is a negative regulator of cellular proliferation stimulated by EGF- and Ras-mediated activation of the ERK pathway PMID: 19022560
Role of NM23-H2 in the regulation of cell shape and migration via Rho family GTPase signals is reported. PMID: 19381785
Data support the hypothesis that exogenous pDNA binds to cytoplasmic shuttle proteins NM23-H2 and Chx10, and is then translocated to the nucleus using the minimal import machinery. PMID: 19638341

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Subcellular Location

Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle.

Protein Families

NDK family

Tissue Specificity

Isoform 1 and isoform 3 are ubiquitously expressed.

Database Links

HGNC: 7850

OMIM: 156491

KEGG: hsa:4831

STRING: 9606.ENSP00000376886

UniGene: Hs.463456

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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