Product Details

Uniprot No.

Target Names

Alternative Names

Glucose sensitive factor antibody; Glucose-sensitive factor antibody; GSF antibody; IDX 1 antibody; IDX-1 antibody; IDX1 antibody; Insulin promoter factor 1 antibody; Insulin promoter factor 1 homeodomain transcription factor antibody; Insulin upstream factor 1 antibody; IPF 1 antibody; IPF-1 antibody; IPF1 antibody; Islet/duodenum homeobox 1 antibody; Islet/duodenum homeobox-1 antibody; IUF 1 antibody; IUF-1 antibody; IUF1 antibody; MODY4 antibody; Pancreas/duodenum homeobox 1 antibody; Pancreas/duodenum homeobox protein 1 antibody; pancreatic and duodenal homeobox P antibody; PDX 1 antibody; PDX-1 antibody; PDX1 antibody; PDX1_HUMAN antibody; Somatostatin transactivating factor 1 antibody; Somatostatin-transactivating factor 1 antibody; STF 1 antibody; STF-1 antibody; STF1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PDX1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4A3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PDX1, also known as pancreas/duodenum homeobox protein 1, serves as a master transcription factor essential for pancreatic development and beta cell function. This homeodomain protein regulates insulin gene expression and plays a critical role in glucose homeostasis, making it a key target for researchers investigating diabetes, pancreatic differentiation, and metabolic disorders. Mutations in PDX1 are associated with maturity-onset diabetes of the young type 4 (MODY4), underscoring its clinical significance in understanding glucose regulation pathways.

This recombinant monoclonal antibody, clone 4A3, offers the reproducibility and consistency that demanding research applications require. Generated against a synthesized peptide from human PDX1 protein and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across studies.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis in COLO205 whole cell lysate detects a band at approximately 42 kDa, which differs from the predicted 31 kDa molecular weight. This size shift is commonly attributed to post-translational modifications, particularly glycosylation and phosphorylation events known to occur on PDX1. Immunohistochemistry validation in paraffin-embedded human pancreatic tissue and gastric cancer samples confirms reliable tissue staining at 1:100 dilution. Flow cytometry analysis using HeLa cells further demonstrates intracellular detection capability with clear signal separation from isotype controls.

Whether you are investigating signal transduction pathways, characterizing pancreatic cell populations, or exploring PDX1 expression in disease contexts, this affinity-purified antibody delivers the performance and consistency needed for publication-quality results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Gene References into Functions

heat shock proteins are involved in PDX1-mediated effects on the genesis of gastric cancer PMID: 30310259
Investigated the effect of gestational diabetes mellitus (GDM) on the expression and methylation of PGC-1alpha and PDX1 in placenta and their effects on fetal glucose metabolism. Blood glucose was negatively correlated with the expression of PGC-1alpha and PDX1 mRNA in the placenta. PMID: 29730292
PDX1, Neurogenin-3, and MAFA are critical transcription regulators for beta cell development and regeneration. (Review) PMID: 29096722
study provides a novel culture protocol for generating pancreas-committed cells from hAECs and reveals an interplay between Pdx1 and activin A/nicotinamide signaling in early pancreatic fate determination. PMID: 28632450
Data indicate the secretory granule membrane glycoprotein 2 as a marker for PDX1+/NKX6-1+ pancreatic progenitors (PPs). PMID: 28835709
expression of regulatory master genes of embryonic development in pancreatic cancer cells on the intracellular concentration of the master regulator PDX1 PMID: 28864896
Efficiency of differentiation of Induced pluripotent stem cells to insulin producing cells can be increased by concurrent expression of PDX1 and NKX6.1 during progenitor cells maturation. PMID: 27998294
Mutation in PDX1 gene is associated with Maturity Onset Diabetes of the Young. PMID: 28095440
Results found that Pdx-1 overexpression exerts its effects on islet cell replication via a noncell-autonomous mechanism involving induction of a secreted soluble factor or factors. Also, Inhbb is identified as one factor mediating the proliferative effect of Pdx-1. PMID: 27620967
Our findings indicate that MET induces the differentiation of Pdx1-expressing cells within the definitive endoderm in a time-dependent manner, and suggest useful application for regenerative medicine. PMID: 28202025
Pdx1 and MafA play crucial roles in the pancreas and maintain mature beta-cell function. Our results showed that the expression of Pdx1 and MafA were significantly upregulated after a sleeve gastrectomy for morbid obesity. PMID: 26781599
Data show that the expression patterns of sex determining region Y-box 2 (SOX2) and pancreatic and duodenal homeobox 1 (Pdx1)) highly correlated with prognosis of pancreatic neuroendocrine tumors (p-NETs). PMID: 26491904
The ERK-PDX-1 interaction in PANC-1 cells. PMID: 26695692
Foxo1 and PDX1 are colocalized in fetal pancreatic islets, suggesting they function in parallel in beta cells. PMID: 26727107
This study investigated the role of Pdx1 and its mechanism in differentiating induced pluripotent stem cells (iPSCs) into islet beta cells. PMID: 26345820
the human Pdx1 is highly effective in triggering human biliary tree stem cells differentiation toward functional beta-pancreatic cells. PMID: 26252949
Introduction of the PDX1 gene makes cells acquire a functional insulin secretory capacity PMID: 25817342
These results suggest that IFN-gamma induced beta-cell dysfunction is mediated through decreased nuclear localization of Pdx-1. PMID: 25839229
The rs12255372 SNP of TCF7L2 and D76N of PDX-1 genes may confer susceptibility to T2DM in the population living in Mashhad, Iran. PMID: 26058934
Pdx1 has a role in pancreatic differentiation in human amniotic fluid-derived stem cells PMID: 23147868
physiological and pathophysiological conditions dynamically regulated Pdx1 binding to these Swi/Snf complexes in vivo PMID: 25801033
we found that PDX1 binds and suppresses expression of endogenous liver genes. These findings rebrand PDX1 as a context-dependent transcriptional repressor and activator within the same cell type. PMID: 25843046
PDX-1mRNA is detectable in samples of pancreatic cancer. Its quantification may be helpful to improve the diagnosis of pancreatic cancer. PMID: 25454709
These results indicate that PDX-1 mRNA may offer a promising approach to produce safe IPCs for clinical diabetes mellitus treatment. PMID: 25201603
CONCLUSIONS: Our results demonstrate the use of in vitro-synthesized PDX1 messenger RNA to differentiate hUC-MSCs into islet-like cells and pave the way toward the development of reprogramming and directed-differentiation methods PMID: 25287601
MAFA, MAFB, NKX6.1, and PDX1 activity provides a gauge of islet beta cell function, with loss of MAFA (and/or MAFB) representing an early indicator of beta cell inactivity PMID: 23863625
A rare (0.20%) frameshift variant in PDX1, encoding p.Gly218Alafs*12, associates with high risk of type 2 diabetes (OR = 2.27, P = 7.3 x 10(-7)). PMID: 24464100
Performed fully atomistic Molecular Dynamics simulations both in crystal and aqueous environments in order to elucidate the nature of the difference in binding contacts between Pdx1 and DNA (PDB 2H1K) . PMID: 23950697
Developed a liquid-phase, non-radioactive, sensitive and specific luciferase immunoprecipitation systems assay to detect pdx1 autoantibodies in human sera. PMID: 23696946
Pdx1 is a transcription factor required for the formation of endocrine pancreas that is inhibited during Coxsackievirus B4 infection of human pancreas ductal cells PMID: 23775130
recessive PDX1 mutations are a rare but important cause of isolated permanent neonatal diabetes in patients without pancreatic hypoplasia/agenesis PMID: 23320570
PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells. PMID: 23853095
studies indicated p38 MAP kinase is a positive regulator of PDX-1 stability and p38 exerts its stabilizing effect on PDX-1 through a phosphorylation-dependent inhibition of PDX-1 ubiquitination. PMID: 23331010
PDX-1 is a therapeutic target for both hormonal symptoms and tumor volume in models of pancreatic cancer, insulinoma and islet neoplasia PMID: 22905092
Up-regulation of Pdx1 is a feature of cholangiocarcinogenesis associated with chronic cholangitis. PMID: 22594685
data demonstrate epigenetic modifications of PDX-1 may reduce its expression in diabetic islets which may lead to impaired insulin expression and secretion; data further suggest hyperglycemia may be a factor behind increased DNA methylation and decreased expression of PDX-1 PMID: 22570331
Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID: 22761608
PDX1 repression of FABP1, LCT, and SI suggests a role for PDX1 in patterning anterior intestinal development. PMID: 22640736
Analysis of cell death genes led to the identification of Noxa and Puma as direct targets of Pdx1 PMID: 22322596
Through multiple mechanisms, SREBP-1c, when induced in a lipotoxic state, repressed PDX-1 expression contributing to the inhibition of insulin expression and beta cell dysfunction. PMID: 21652712
a significant relation of PDX1 and CDX2 expressions among phenotypic classification of gastric carcinomas reveals an idea about similar functions for PDX1 and CDX2 in the evolution of gastric carcinoma. PMID: 21909647
PDX-1 plays an important role in the induction of CD44+/CD105+ human amniotic fluid cells (HuAFCs) into functional pancreatic beta-cell-like cells in vitro. PMID: 21612404
Gastrin-positive neuroendocrine tumors, whether of duodenal or pancreatic origin, frequently expressed PDX1 (17/18), ISL1 (14/18), and NGN3 (14/18). PMID: 21739268
PDX1, important for the regulation of insulin metabolism regulation, was able to repress the TBX15 promoter in a methylation-dependent manner, which might, at least partially, explain the specific mRNA decrease of TBX15 observed in vIUGR placentas. PMID: 20962579
ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
pdx-1 functions as a "pancreas-permitting gene". PMID: 20668890
Data suggest that NKX6.1 activates immature pancreatic markers but not pancreatic hormone gene expression in human liver cells, and suggest a potential role for NKX6.1 in promoting PDX-1 reprogrammed maturation along a beta-cell-like lineage. PMID: 21108535
TFF1, TFF2, and PDX1 were expressed only in lobular endocervical glandular hyperplasia. PMID: 21228366
Results indicate that beta-cell dysfunction during progression of CP correlates with the decrease in PDX-1 gene expression. PMID: 20467340
in adult mouse beta cells, Pcif1 limits Pdx1 protein accumulation and thus the expression of insulin and other gene targets important in the maintenance of beta cell mass and function. PMID: 20811152

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Involvement in disease

Pancreatic agenesis 1 (PAGEN1); Diabetes mellitus, non-insulin-dependent (NIDDM); Maturity-onset diabetes of the young 4 (MODY4)

Subcellular Location

Nucleus. Cytoplasm, cytosol.

Protein Families

Antp homeobox family, IPF1/XlHbox-8 subfamily

Tissue Specificity

Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

Database Links

HGNC: 6107

OMIM: 125853

KEGG: hsa:3651

STRING: 9606.ENSP00000370421

UniGene: Hs.32938

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Code	CSB-RA820141A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in: COLO205 whole cell lysate(30µg) All lanes: PDX 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 31 kDa Observed band size: 42 kDa Exposure time：120s IHC image of CSB-RA820141A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA820141A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA820141A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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PDX1 Recombinant Monoclonal Antibody

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