Product Details

Uniprot No.

Target Names

Alternative Names

Phosphatidylethanolamine-binding protein 1 (PEBP-1) (HCNPpp) (Neuropolypeptide h3) (Prostatic-binding protein) (Raf kinase inhibitor protein) (RKIP) [Cleaved into: Hippocampal cholinergic neurostimulating peptide (HCNP)], PEBP1, PBP PEBP

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4F4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PEBP1, also known as Raf kinase inhibitor protein (RKIP), serves as a critical modulator of multiple signaling cascades including the MAPK/ERK pathway, making it a compelling target for researchers investigating metastasis suppression, neuronal function, and cellular signaling dynamics. This protein's dual role as both a kinase inhibitor and the precursor to hippocampal cholinergic neurostimulating peptide (HCNP) positions it at the intersection of cancer biology and neuroscience research.

This recombinant rabbit monoclonal antibody, clone 4F4, offers the reproducibility and batch-to-batch consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, you can expect reliable performance across long-term studies and multi-site collaborations without the variability inherent to traditional hybridoma-derived reagents.

Validation data demonstrates robust performance across diverse experimental platforms. Western blot analysis confirms specific detection at the expected 21 kDa molecular weight across human cell lines including HeLa, HepG2, HEK293, U-251MG, and SH-SY5Y, with cross-species reactivity extending to mouse and rat brain tissue lysates. Immunohistochemistry staining has been validated in human prostate cancer and liver tissue sections, while immunofluorescence studies in HepG2 cells reveal clear cytoplasmic localization patterns. Flow cytometry analysis in PC-3 cells further demonstrates the antibody's utility for single-cell applications.

The unconjugated format and affinity-purified preparation provide flexibility for researchers to pair this antibody with their preferred detection systems. Whether you're exploring PEBP1's role in tumor metastasis, investigating MAPK pathway regulation, or studying cholinergic neurostimulation, this antibody delivers the specificity and versatility your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.; HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.

Gene References into Functions

Low RKIP Expression is associated with asthma. PMID: 30282734
our studies demonstrate how KRAS inhibits the tumor suppressor RKIP, thus offering novel justification for targeting RKIP as a strategy to overcome KRAS-induced tumor metastasis and chemoresistance in PDAC. PMID: 29315556
RKIP expression in gastrointestinal stromal tumors is associated with tumor size, NIH risk grade, and mucosal invasion, and low or no expression of RKIP predicts a high malignancy potential. PMID: 29930472
Octreotide may weaken invasion and metastasis through the upregulation of PEBP1. Octreotide may reduce the risk of recurrence and metastasis after surgery for liver cancer. PMID: 29990987
Knockdown of RKIP increased phosphorylated ERK and thus suppressed sorafenib-mediated cell death. PMID: 29858683
Baseline urinary RKIP, evaluated in an independent cohort of 56 Clear cell Renal Cell Carcinoma patients and 28 healthy subjects, successfully distinguished both groups and, most importantly, a cut-off value of 10 ng/mg/g Pr/uCr enabled a highly accurate prediction of Cancer-specific survival and Progression-free survival. PMID: 28418894
we found that the RKIP expression was decreased significantly in HBV infected tumor tissues compared to normal adjacent tissues, suggesting the reduction or absence of RKIP expression was closely related to liver carcinogenesis. PMID: 27902472
downregulation of RKIP plays an important role in the breast neoplastic progression and correlates with poor prognosis in patients with breast carcinoma (BC). Aberrant RKIP methylation is one of the mechanisms that lead to downregulation of RKIP in BC. PMID: 26894644
can act as a negative mediator in autophagy through stimulation of the AKT-MTORC1 pathway and direct interaction with LC3. PMID: 27540684
PEBP1, a scaffold protein inhibitor of protein kinase cascades, complexes with two 15LO isoforms, 15LO1 and 15LO2, and changes their substrate competence to generate hydroperoxy-phosphatidylethanolamines (PE). Inadequate reduction of hydroperoxy-PE due to insufficiency or dysfunction of a selenoperoxidase, GPX4, leads to ferroptosis. PMID: 29053969
miR-23a as a negative regulator of RKIP expression in AML. PMID: 27197200
Knockdown of RKIP promotes LX-2 cell proliferation. PMID: 28031115
RKIP contributes to colitis development by promoting inflammation and mediating intestinal epithelial cell apoptosis. PMID: 26801887
The study demonstrates that miR-543 promotes the proliferation and metastasis of prostate cancer via targeting RKIP. PMID: 28245474
RKIP inhibitors locostatin reduces extracellular matrix production as well as the migration and proliferation of myometrial and leiomyoma cells. PMID: 27565262
RKIP has a role in suppressing proliferation and metastasis of breast cancer cell lines through up-regulation of miR-185 targeting HMGA2 PMID: 27651238
positive p-Ser153 RKIP expression is a favorable prognostic factor and affects clinical response to radiotherapy in nasopharyngeal carcinoma PMID: 27647315
Expression of RKIP is closely correlated with the survival of pancreatic cancer patients. RKIP can inhibit pancreatic adenocarcinoma cells proliferation, activities of migration and invasion, through downregulating Raf-1-MEK1/2-ERK1/2 signaling pathway. PMID: 27444299
RKIP expression is negatively associated with tumor metastasis in gastric cancer. PMID: 25764514
Authors demonstrate that the inhibition of the CCL5 expression is the cause of the observed effects resulting from RKIP expression. Results underscore the significance of RKIP as important negative regulator of tumor microenvironment. PMID: 26375811
report of the expression profile of RKIP in a heterogeneous group of soft tissue sarcomas; findings showed that only a small subset of cases exhibited RKIP loss, yet its loss is an independent biomarker of prognosis PMID: 26859895
RKIP inhibits glioma cell migration and invasivess by inhibiting MMP2/MMP9/HMAG2 expression. PMID: 26823735
Loss of RKIP is associated with neoplasms. PMID: 26716415
Downregulation or depletion of RKIP was related to the onset and progression of esophageal squamous cell carcinoma, and facilitated the invasion and metastasis of ESCC by downregulating E-cadherin and upregulating NF-kB p65. PMID: 26617724
Identify intestinal RKIP as capable of hydrolyzing prasugrel to its thiolactone metabolite. PMID: 26558823
Study indicates that reduced RKIP expression is correlated with metastasis and recurrence of disease in stage II colorectal cancer (CRC) suggesting it as an important metastasis-suppressor gene in CRC. PMID: 26238523
RKIP Inhibits Local Breast Cancer Invasion by Antagonizing the Transcriptional Activation of MMP13 PMID: 26308852
Results suggest that up-regulation of miR-27a could suppress RKIP expression and in turn contribute to chemoresistance of lung adenocarcinoma cells to cisplatin. PMID: 25128483
Raf kinase inhibitor protein mediated signaling inhibits invasion and metastasis of hepatocellular carcinoma. PMID: 26116914
RKIP is a metastatic suppressor and predictor for metastasis and prognosis in nasopharyngeal carcinoma, and RKIP downregulation promotes nasopharyngeal carcinoma invasion, metastasis and epithelial to mesenchymal transition by activating Stat3 signaling PMID: 25915430
The upregulated expression of RKIP in colorectal cancer cells inhibited cell invasion and metastasis, while downregulation of RKIP reduced chemosensitivity by inhibiting apoptosis induced by HCPT. PMID: 26177829
This study showed that elevated amygdala HCNP-pp expression may contribute to mechanisms of major depression in women. PMID: 25819500
RKIP is upregulated in heart failure. PMID: 26479924
In triple negative breast cancer, RKIP regulates tumor-associated macrophage recruitment by blocking HMGA2, resulting in reduced expression of numerous macrophage chemotactic factors, including CCL5. PMID: 26238785
downregulation of RKIP occurs frequently... suggesting RKIP being a critical tumor suppressor for clear cell renal cell carcinomas PMID: 25277181
Loss of RKIP expression was associated with unfavorable OS. PMID: 25171481
Data suggest that RKIP inhibits gastric cancer metastasis via the downregulation of its downstream target genes STAT3 and cyclin D1. PMID: 25337233
functional RKIP rs17512051 polymorphism may affect ccRCC susceptibility PMID: 25329396
Raf kinase inhibitor protein expression is a suitable and easily determinable marker in the primary tumour that could predict the risk of uveal melanoma to metastasize PMID: 24763848
Authors demonstrate that the N-terminus of human Raf1 kinase (hRaf11-147aa) binds with human RKIP (hRKIP) at its ligand-binding pocket, loop "127-149", and the C-terminal helix by nuclear magnetic resonance experiments. PMID: 24863296
RKIP inhibits gastric cancer cell survival and invasion by regulating the expression of HMGA2 and OPN. PMID: 25172097
Fine control of RKIP activity and regulation is crucial to avoid pathological processes, such as metastasis,pulmonary arterial hypertension, and heart failure.[review] PMID: 25597351
Genetic an epigenetic control of RKIP transcription and review of known mechanisms employed by different types of chemotherapeutic agents to activate RKIP expression in cancer cells.[review] PMID: 25597352
This brief review describes the role of RKIP in the regulation of drug sensitivity via disruption of the NF-kappaB/Snail/ YY1/RKIP loop that regulates resistance in cancer cells. [review] PMID: 25597353
Here, we review RKIP's role as a metastasis suppressor and the signaling networks and genes regulated by RKIP in metastatic, triple-negative breast cancer. [review] PMID: 25597354
RKIP loss is a frequent occurrence in many solid tumor cancers and may serve as a viable prognostic biomarker. [review] PMID: 25597355
Here, we present a three-state model to explain the RKIP functional switch and discuss the role of the pocket loop in regulating RKIP activity. [review] PMID: 25597357
RKIP synergizes with microtubule inhibitors to induce apoptosis and inhibit STAT3 activation of breast and prostate cancer cells. RKIP plays a critical role in opposing the effects of pro-oncogenic STAT3 activation. PMID: 24658061
overexpression of RKIP could decrease the proliferation and increase the apoptosis of K562 cells. PMID: 25015191
down-regulation of RKIP expression was observed in human gastric cell lines, and miR-224 could negatively regulate the expression and biological characteristics of RKIP, contributing to suppress the proliferation and invasion of gastric cells. PMID: 25017365

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Subcellular Location

Cytoplasm.

Protein Families

Phosphatidylethanolamine-binding protein family

Database Links

HGNC: 8630

OMIM: 604591

KEGG: hsa:5037

STRING: 9606.ENSP00000261313

UniGene: Hs.433863

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Code	CSB-RA903387A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: PEBP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time：1min IHC image of CSB-RA903387A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA903387A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA903387A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing PC-3 cells stained with CSB-RA903387A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PEBP1 Recombinant Monoclonal Antibody

Product Details

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Target Background