PHB2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA212222A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg), A431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Mouse lung tissue lysate(30µg), Mouse kidney lysate(30µg) All lanes: Prohibitin 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time：5s IHC image of CSB-RA212222A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA212222A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA212222A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA212222A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q99623

Target Names

PHB2

Alternative Names

B cell receptor associated protein BAP37 antibody; B-cell receptor-associated protein BAP37 antibody; BAP antibody; Bap37 antibody; BCAP 37 antibody; D prohibitin antibody; D-prohibitin antibody; p22 antibody; Phb2 antibody; PHB2_HUMAN antibody; PNAS 141 antibody; Prohibitin 2 antibody; Prohibitin-2 antibody; Repressor of estrogen receptor activity antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human PHB2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3A4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Prohibitin 2 (PHB2) serves as a critical regulator of cellular homeostasis, functioning as a mitochondrial chaperone and transcriptional modulator with established roles in estrogen receptor signaling, mitochondrial dynamics, and cell proliferation. Also known as BAP37 and repressor of estrogen receptor activity, this protein has garnered significant attention in cancer biology and metabolic research, making reliable detection tools essential for investigators exploring these pathways.

This recombinant monoclonal antibody, clone 3A4, offers the reproducibility that demanding research requires. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit host and monoclonal nature ensure consistent specificity against human PHB2, with validated cross-species reactivity extending to mouse samples.

Extensive validation demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis confirms detection of the expected 33 kDa band across diverse human cell lines including HeLa, HepG2, A549, A431, COLO205, SH-SY5Y, and HEK293, as well as mouse lung and kidney tissue lysates, providing confidence when working with different model systems. Immunohistochemistry applications have been validated in human heart tissue and colorectal cancer specimens, supporting both normal physiology and disease-focused investigations. Immunofluorescence staining in HepG2 cells and flow cytometry analysis in HeLa cells further expand the experimental possibilities, enabling subcellular localization studies and quantitative single-cell analyses.

Whether investigating mitochondrial function, estrogen receptor regulation, or signal transduction mechanisms in cancer models, this antibody provides the technical foundation for generating reliable, publication-quality data across your preferred experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus.; In the mitochondria, together with PHB, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Also regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity. Has a key role of mitophagy receptor involved in targeting mitochondria for autophagic degradation. Involved in mitochondrial-mediated antiviral innate immunity, activates DDX58/RIG-I-mediated signal transduction and production of IFNB1 and proinflammatory cytokine IL6.; In the nucleus, serves as transcriptional co-regulator (Probable). Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity.; In the plasma membrane, is involved in IGFBP6-induced cell migration. Cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.; (Microbial infection) Involved in human enterovirus 71/EV-71 infection by enhancing the autophagy mechanism during the infection.

Gene References into Functions

High PHB2 expression is associated with high ribosomal RNA transcription and facilitation of cell proliferation in rhabdomyosarcoma. PMID: 29367618
the present study suggested that PHB2 may promote Prostate cancer cell migration by inhibiting the expression of AKT2. These results provide information regarding the role of PHB2 in Prostate cancer migration and malignancy PMID: 29207197
Taken together, the results indicate that PHB2 plays a central role in p21 upregulation following GGCT knockdown and as such may promote deregulated proliferation of cancer cells by suppressing p21. PMID: 29307834
analysis of LGALS3, PHB2, MUC1, and GK2 expression with CA15-3 in early-stage breast cancer PMID: 26289852
REA modulates cross talk among multiple cell types in the uterine tissue and host background, serving as a brake on the estradiol-ER axis and restraining multiple aspects that contribute to the pathologic progression of endometriosis. PMID: 26653759
results show that PHB2 binds to the ligand binding domain of ERalpha with a conformational change in the helix 12 of ERalpha PMID: 26049107
Functional analysis of selected regulated proteins revealed that knockdown of HNRPD, PHB2 and UB2V2 can increase HCMV replication, while knockdown of A4 and KSRP resulted in decreased HCMV replication. PMID: 25910425
These results demonstrate that estradiol upregulates REA expression and recruits REA via ERalpha to the EREs on the RORgammaT promoter region, thus inhibiting RORgammaT expression and Th17 differentiation. PMID: 25769926
BIG3 (ARFGEF3) is predicted to interact with its partner PHB2 through an ARM-type alpha-helical structure. PMID: 24997568
Data indicate that the up-regulated expression of prohibitin promoted acute promyelocytic leukemia cell line NB4-R1 cell apoptosis. PMID: 25200157
PHB2 in hepatocellular carcinoma supports the development and progression of hepatocellular malignancy. PMID: 23661548
Prohibitin 2 acts as a nuclear AKT substrate during all-trans retinoic acid-induced differentiation of acute promyelocytic leukemia cells PMID: 24522204
prohibitin and prohibiton (PHB2) contribute to PIG3-mediated apoptosis by binding to the PIG3 promoter (TGYCC)15 motif PMID: 24388982
Data indicate that IGFBP-6 binds to prohibitin-2 on the cell membrane, and knockdown of the latter abrogates IGFBP-6-induced migration. PMID: 24003225
Data demonstrate that PHB2 is essential for metabolic activation of mitochondria and, as a consequence, for function and survival of beta-cells. PMID: 23863811
ASURA specifically binds to chromatin when Scc1 is associated with chromatin. PMID: 23548868
PHBs are localized on the human platelet membrane and are involved in PAR1-mediated platelet aggregation. PMID: 22212092
REA physiologically restrains endometrial stromal cell decidualization, controlling the timing and magnitude of decidualization to coordinate uterine differentiation with concurrent embryo development that is essential for implantation and fertility. PMID: 23392257
Casein kinase 1 proteomics reveal prohibitin 2 function in molecular clock PMID: 22384121
Prohibitin 1 and 2 are required for cancer cell proliferation and adhesion. PMID: 20856874
results point to binding of the Phb1/Phb2 complex to the Env-CT as being of importance for replicative spread in nonpermissive cells, possibly by modulating critical Phb-dependent cellular process(es) PMID: 19906925
REA has a role as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases PMID: 15140878
mammalian PHB2 has roles in mitochondria and its nuclear translocation is estrogen receptor-dependent PMID: 17008324
EZH2 regulates the transcription of estrogen-responsive genes through association with REA, an estrogen receptor corepressor PMID: 17453341
Findings suggest that, in addition to the shugoshin, PHB2 is also required to protect the centromeric cohesion from phosphorylation by Plk1 during early mitosis and that its function is essential for proper mitotic progression. PMID: 17656096
SKP2 variant Skp2B interacts with the repressor of estrogen receptor activity (REA) and that overexpression of Skp2B leads to a reduction in REA levels. PMID: 17785450
Results demonstrate that the repressor of estrogen receptor activity (REA), a protein related to PHB, interacts with PHB, to form heteromers and enhance the protein stability of both corepressors. PMID: 17932104
Phb1 and Phb2 are novel phosphoproteins up-regulated during T cell activation that function to maintain mitochondrial integrity PMID: 18086671
a novel mechanism by which RNF2 and PHB2 modulate the CP2-mediated transcriptional pathway. PMID: 18629613
Estradiol downregulation of the tumor suppressor gene BTG2 requires estrogen receptor-alpha and the REA corepressor PMID: 19117054
Severe acute respiratory syndrome coronavirus nonstructural protein 2 interacts with a host protein complex of PHB1 and PHB2 involved in mitochondrial biogenesis and intracellular signaling PMID: 19640993

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Subcellular Location

Mitochondrion inner membrane. Cytoplasm. Nucleus. Cell membrane.; [Isoform 1]: Mitochondrion inner membrane.; [Isoform 2]: Mitochondrion inner membrane.

Protein Families

Prohibitin family

Database Links

HGNC: 30306

OMIM: 610704

KEGG: hsa:11331

STRING: 9606.ENSP00000441875

UniGene: Hs.504620

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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