PPA1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA614884MA1HU
Size	US$210
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Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(20µg), HEK293 whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), K562 whole cell lysate(20µg), U-251MG whole cell lysate(20µg), HepG2 whole cell lysate(20µg), Jurkat whole cell lysate(20µg), A549 whole cell lysate(20µg), HeLa whole cell lysate(20µg) All lanes: PPA1 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time：5s Western Blot Positive WB detected in: Mouse small intestine tissue lysate(20µg), Rat small intestine tissue lysate(20µg) All lanes: PPA1 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size: 32.7 kDa Observed band size: 33 kDa Exposure time: 2min Overlay Peak curve showing HEK293 cells stained with CSB-RA614884MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q15181

Target Names

PPA1

Alternative Names

cytosolic inorganic pyrophosphatase antibody; diphosphate phosphohydrolase antibody; inorganic diphosphatase antibody; inorganic pyrophosphatase 1 antibody; Inorganic pyrophosphatase antibody; IOPPP antibody; IPYR_HUMAN antibody; PP antibody; PP1 antibody; PPA1 antibody; PPase antibody; Pyp antibody; pyrophosphatase, inorganic, 1 antibody; Pyrophosphate phospho hydrolase antibody; Pyrophosphate phospho-hydrolase antibody; SID6-8061 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human PPA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

1H4E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Inorganic pyrophosphatase 1 (PPA1) serves as a critical enzyme in cellular energy metabolism, catalyzing the hydrolysis of inorganic pyrophosphate to drive biosynthetic reactions forward. This housekeeping enzyme plays an essential role in nucleic acid synthesis, protein production, and lipid metabolism, making it a valuable marker for studying metabolic regulation and cellular proliferation. Emerging research has also linked PPA1 expression to tumor progression and metabolic reprogramming in cancer cells, positioning it as a target of growing interest in oncology research.

This recombinant monoclonal antibody against PPA1 offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The human IgG1 format provides reliable performance across detection platforms while maintaining specificity for your target.

Validation studies demonstrate robust performance across multiple applications. In western blotting, this antibody detects PPA1 at the expected 33 kDa molecular weight across a diverse panel of human cell lines including HT-29, HEK293, MCF-7, K562, U-251MG, HepG2, Jurkat, A549, and HeLa cells. Cross-species reactivity extends to rodent samples, with confirmed detection in mouse and rat small intestine tissue lysates, supporting translational research using animal models. Flow cytometry validation in HEK293 cells demonstrates clear separation from isotype control, enabling intracellular protein quantification studies.

Whether investigating metabolic enzyme regulation, characterizing cancer cell phenotypes, or exploring pyrophosphate-dependent pathways, this antibody provides the specificity and cross-species reactivity needed to advance your research across multiple experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

The p37 controls cortical NuMA levels via the phosphatase PP1 and its regulatory subunit Repo-Man, but it acts independently of Galphai, the kinase Aurora A, and the phosphatase PP2A. PMID: 29222185
Proteomic profiling identifies the inorganic pyrophosphatase (PPA1) protein as a potential biomarker of metastasis in laryngeal squamous cell carcinoma. PMID: 26948660
Results show that PPA1 expression is significantly higher in many tumors, especially those of lung and ovarian origin, which suggests that PPA1 plays an important role in carcinogenesis and in the development of some tumors. PMID: 27666431
PPA1 serves as a potential prognostic biomarker for patients with Ovarian serous carcinoma. PMID: 28202851
Thus, the Ska complex, specifically the Ska1 C-terminal domain, recruits PP1 to kinetochores to oppose spindle checkpoint signaling kinases and promote anaphase onset. PMID: 26981768
PPA1 may serve as a potential biomarker of poor prognosis in patients with gastric cancer PMID: 26722435
PP-1 and Akt have roles in AR-v7 protein expression and activities when AR is functionally blocked PMID: 26378044
The Newcastle disease virus-induced translation shutoff at late infection times was attributed to sustaining phosphorylation of eIF2a, which is mediated by continual activation of PKR and degradation of PP1. PMID: 26869028
The human PP1 interaction with the measles V protein is mediated by a conserved PP1-binding motif in the C-terminal region of the V protein. PMID: 25011105
Measles virus binding to DC-SIGN leads to inhibition of PP1 phosphatase activity and hence inhibition of RIG-I and Mda5 dephosphorylation. PMID: 25011106
Results identify PP1 as a novel regulator of Cl(-)-HCO3(-) anion exchangers activity which, in concert with CFTR, coordinates events at both apical and basolateral membranes, crucial for efficient HCO3(-) secretion from Calu-3 cells. PMID: 23215877
PPP1R42 regulation of PP1 function in centrosome separation. PMID: 23718219
Pyrophosphatase overexpression is associated with invasion, metastasis and recurrence in gastric cancer PMID: 22797819
PP1gamma is the major histone H3 phosphatase acting on the mitotically phosphorylated residues H3T3ph, H3S10ph, H3T11ph, and H3S28ph. PMID: 21514157
IRBIT opposes the effects of WNKs and SPAK by recruiting PP1 to the complex to dephosphorylate CFTR and NBCe1-B, restoring their cell surface expression, in addition to stimulating their activities PMID: 21317537
Data are consistent with the postsynaptic gephyrin scaffold acting as a platform for protein phosphatase 1 (PP1), which regulates gephyrin cluster size by dephosphorylation of gephyrin- or cytoskeleton-associated proteins. PMID: 20206270
Neither human hexokinase-1 nor human inorganic pyrophosphatase expression segregated concordantly with human cytoplasmic glutamic-oxaloacetic transaminase expression. PMID: 17494625

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Subcellular Location

Cytoplasm.

Protein Families

PPase family

Tissue Specificity

Expressed ubiquitously.

Database Links

HGNC: 9226

OMIM: 179030

KEGG: hsa:5464

STRING: 9606.ENSP00000362329

UniGene: Hs.437403

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Related pathways

Myocardial Fatty Acid Metabolism Oxidative Phosphorylation

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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