Product Details

Uniprot No.

Target Names

Alternative Names

CLL associated antigen KW 7; CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGF; Lens epithelium derived growth factor; Lens epithelium-derived growth factor; MGC74712; p52; p75; PAIP; PC4 and SFRS1 interacting protein 1; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1 interacting protein; PC4 and SFRS1-interacting protein; PSIP 1; PSIP 2; Psip1; PSIP1_HUMAN; PSIP2; Transcriptional coactivator p75/p52

Species Reactivity

Human

Immunogen

A synthesized peptide from human PSIP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5H8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PSIP1, also known as LEDGF (Lens Epithelium-Derived Growth Factor) or DFS70, serves as a critical transcriptional coactivator involved in chromatin binding, stress response, and DNA repair pathways. This protein has garnered significant research attention due to its role as a cofactor for HIV integrase, making it a key target in retroviral integration studies. Additionally, PSIP1 has emerged as a clinically relevant autoantigen, with anti-DFS70 antibodies serving as important biomarkers in autoimmune disease diagnostics.

This recombinant rabbit monoclonal antibody (clone 5H8) offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human PSIP1, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Western blot analysis confirms robust detection in human cell lines including HepG2, Jurkat, and HeLa lysates, with an observed band at approximately 75 kDa. This size difference from the predicted 60 kDa molecular weight likely reflects post-translational modifications, particularly glycosylation, characteristic of the native protein. Immunohistochemistry validation in human spleen tissue and colorectal cancer specimens shows clear staining using citrate buffer antigen retrieval, while flow cytometry analysis in SH-SY5Y neuroblastoma cells confirms utility for single-cell detection approaches.

Whether investigating LEDGF's role in HIV integration, exploring its function in transcriptional regulation, or examining its significance as an autoimmune target, this antibody provides a reliable tool for advancing your PSIP1 research across immunoblotting, tissue-based, and cytometric applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.

Gene References into Functions

structurally conserved IBD-binding motifs (IBMs) on known LEDGF/p75 binding partners can be regulated by phosphorylation, permitting switching between low- and high-affinity states. PMID: 29997176
findings suggest a novel role of LEDGF/p75 in protecting the unintegrated 3' processed linear HIV-1 cDNA from exonucleolytic degradation. PMID: 28914817
mono-specific anti-DFS70 antibodies are a strong discriminator between ANA positive healthy controls and patients with systemic autoimmune rheumatic diseases. PMID: 28770702
Authors report here that the endogenous levels of LEDGF/p75 are upregulated in metastatic castration resistant prostate cancer (mCRPC) cells selected for resistance to the taxane drug docetaxel (DTX). PMID: 28212536
An important role of PSIP1 in triple negative breast cancer tumorigenicity by promoting the expression of genes that control the cell cycle and tumor metastasis PMID: 28633434
We identify the Myc-interacting protein JPO2 and its partner binding protein LEDGF/p75 as critical modulators of PI3K/AKT signaling and metastasis in medulloblastoma PMID: 27013196
The association of LEDGF proteins with the FACT complex and give further support to a role of SSRP1 in HIV-1 infection. PMID: 27216501
short isoform (p52) of a transcriptional co-activator-PC4 and SF2 interacting protein (Psip1), which is known to be involved in linking transcription to RNA processing, specifically regulates the expression of the lncRNA Hottip-located at the 5' end of the Hoxa locus PMID: 28384324
Germline polymorphisms in an enhancer of PSIP1 are associated with epithelial ovarian cancer. PMID: 26840454
PSIP1 likely plays an important role in Hereditary Hearing Loss. PMID: 26689366
LEDGF/p75 Overexpression Attenuates Oxidative Stress-Induced Necrosis and Upregulates the Oxidoreductase ERP57/PDIA3/GRP58 in Prostate Cancer PMID: 26771192
common LEDGF/p75 interaction interface shared by JPO2, PogZ, MLL1, IWS1 and HIV IN PMID: 26245978
Results show that these antibodies are highly specific for dense fine speckles 70 protein DFS70/LEDGFp75 and do not target methyl CpG binding protein 2 (MeCP2). PMID: 26235378
LEDGF/p75 interacts with splicing factors, contributes to exon choice, and directs HIV-1 integration to transcription units that are highly spliced. PMID: 26545813
Together, these results provide evidence for an Integrase/Pol mediated uptake of LEDGF/p75 in HIV-1 viral particles and a specific cleavage by HIV protease. PMID: 25809198
Altogether, these results indicate that a complex containing LEDGF/p75, Iws1, and Spt6 participates in regulating postintegration steps of HIV latency. PMID: 25590759
LEDGF and 44 other novel putative nuclear targets of human thioredoxin 1 have been identified. PMID: 25231459
Results of the present work reinforce the association of PSIP1 gene and rs61744944 single nucleotide polymorphism with HIV-1 infection long-term nonprogressors status. PMID: 25047784
Data show that the same site on integrase-binding domain (IBD) of lens epithelium-derived growth factor (LEDGF) is involved in binding to MLL protein and HIV-IN (integrase), indicating an approach to target LEDGF in both leukemia and HIV infection. PMID: 25305204
Authors identified an additional LEDGF/p75-MLL interface, which overlaps with the binding site of known LEDGF/p75 interactors-HIV-1 integrase, PogZ, and JPO2. PMID: 25082813
a regulation of LEDGF interaction with chromatin by cellular partners of its PWWP domain could be involved in several processes linked to LEDGF tethering properties, such as lentiviral integration, DNA repair or transcriptional regulation PMID: 24312278
LEDGF is crucial for the growth and survival of HPV-positive cancer cells PMID: 24604027
Data indicate that JPO2 and LEDGF/p75 interact directly and specifically in vivo through the specific interaction domain of JPO2 and the C-terminal domain of LEDGF/p75. PMID: 24634210
conclude that FBXO10, a candidate breast cancer susceptibility associated gene, is induced by cellular stress and LEDGF may play a role in expression of this gene PMID: 23138933
Data indicate that the stabilized peptides inhibit the interaction of HIV-1 integrase (IN) with the cellular cofactor LEDGF/p75. PMID: 23758584
LEDGF organizes and stabilizes an active integrase tetramer suitable for specific viral DNA integration. PMID: 23593299
Although identified in a cohort of long-term nonprogressors, our study did not indicate that the I436S or T473I mutation in LEDGF/p75 affects the interaction with HIV-1 integrase. PMID: 23211777
Collectively, results reveal the involvement of LEDGF-mediated elevated expression of Hsp27-dependent survival pathway(s) in prostate cancer PMID: 22647853
The observed variation in LEDGF/p75 expression was not correlated with the disease progression. PMID: 23226247
A role for the human immunodeficiency virus type 1 capsid CA protein in determining the dependency of HIV-1 on LEDGF/p75 during infection highlights a connection between the viral capsid and chromosomal DNA integration. PMID: 23097450
Cotransfection studies with Sp1 in Drosophila cells that were Sp1-deficient, showed increased LEDGF/p75 transcription. PMID: 22615874
reduced LEDGF/p75 levels may play a role in resistance to HIV-1 infection. PMID: 22479480
findings provide insights into the regulation and regulatory functions of LEDGF in Sumoylation-dependent transcriptional control that may be essential for modifying the physiology of cells to maintain cellular homeostasis PMID: 22748127
Depletion of LEDGF impairs the recruitment of C-terminal binding protein interacting protein (CtIP) to DNA DSBs and the subsequent CtIP-dependent DNA-end resection. PMID: 22773103
We propose that Psip1/p52, through its binding to both chromatin and splicing factors, might act to modulate splicing PMID: 22615581
The LEDGF/p75-MeCP2 interaction differentially influences Hsp27pr activation depending on the cellular and molecular context. PMID: 22275515
findings suggest that genetic variation in LEDGF may influence susceptibility to HIV-1 infection and disease progression, which provides in-vivo evidence that LEDGF/p75 is an important host cofactor for HIV-1 replication PMID: 22317832
Results further establish LEDGF/p75 as a cancer-related protein, and provide a rationale for ongoing studies aimed at understanding the clinical significance of its expression in specific human cancers. PMID: 22276150
The fusion genes combining NUP98 exon 11/12 with PSIP1 exon 8 may be implicated in the pathogenesis of myelodysplastic syndrome (MDS). PMID: 22103895
P23H rhodopsin as well as WT rhodopsin form aggregates in RPE cells and LEDGF(1-326) decreases these aggregates. Further, LEDGF(1-326) reduces the RPE cell damage caused by P23H rhodopsin. PMID: 21915354
Computational, biochemical, and genetic approaches enabled us to identify the transcription factor Sp1 as a key modulator of the PSIP1 promoter, controlling LEDGF/p75 transcription through two binding sites PMID: 22019592
LEDGF/DFS70 activates the MK2/IL6/STAT3 pathway in HaCaT keratinocytes. PMID: 21676593
We conclude that serum IgE-anti-DFS70 autoantibodies (aAbs) and IgG(4)-anti-DFS70 aAbs may be related to the severity of atopic dermatitis. PMID: 21329475
Association of polymorphisms in the LEDGF/p75 gene (PSIP1) with susceptibility to HIV-1 infection and disease progression may affect patients outcomes. PMID: 21681054
We confirm that p75 is useful in differentiating atypical fibroxanthoma from spindle cell melanoma. PMID: 21623867
The memory subset of CD4+ T cells showed significantly higher expression levels of APOBEC3G, TRIM5alpha, and LEDGF/p75. PMID: 21784078
The effect of HIV-1 integrase on the chromatin binding of LEDGF/p75 requires the direct interaction of these two proteins. PMID: 21510906
LEDGF dominant interference and depletion impair HIV-1 integration at distinct postentry stages. PMID: 21270171
ectopic expression of LEDGF/DFS70 in keratinocytes could be involved in the pathology of psoriasis vulgaris PMID: 20631726
It appears that the lack of integration observed in HIV-1 infected LEDGF/p75-knockdown cells is due mainly to the inhibitory effect of Rev following the formation of a Rev-integrase complex. PMID: 20678206

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Involvement in disease

A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1 exon 4.

Subcellular Location

Nucleus. Note=Remains chromatin-associated throughout the cell cycle.

Protein Families

HDGF family

Tissue Specificity

Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), i

Database Links

HGNC: 9527

OMIM: 603620

KEGG: hsa:11168

STRING: 9606.ENSP00000370109

UniGene: Hs.658434

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Code	CSB-RA275990A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Hela whole cell lysate(30µg) All lanes: PSIP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time：1min IHC image of CSB-RA275990A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA275990A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA275990A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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PSIP1 Recombinant Monoclonal Antibody

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