PSMA1 Recombinant Monoclonal Antibody

PSMA1 (Proteasome subunit alpha type-1) serves as a core component of the 20S proteasome, the catalytic machinery responsible for regulated protein degradation in eukaryotic cells. This proteolytic complex plays essential roles in maintaining cellular homeostasis, regulating cell cycle progression, and modulating immune responses through antigen processing. Dysregulation of proteasome function has been implicated in numerous pathological conditions, including cancer and neurodegenerative diseases, making PSMA1 a valuable target for researchers investigating protein quality control mechanisms and therapeutic interventions.

This recombinant monoclonal antibody, clone 16H4, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human PSMA1 and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring reliable results across extended studies and multi-site collaborations.

Validation data demonstrates robust performance across multiple detection platforms. Immunohistochemistry studies using human colorectal cancer and small intestine tissue sections show clear target detection at 1:100 dilution following citrate buffer antigen retrieval. Immunofluorescence analysis in PC-3 prostate cancer cells reveals distinct cytoplasmic staining patterns consistent with proteasome localization, while flow cytometry experiments using HepG2 hepatocellular carcinoma cells confirm specific signal separation from isotype controls. This versatility across tissue sections, adherent cell lines, and suspension-based assays provides researchers with flexibility in experimental design.

Whether investigating proteasome biology in cancer models, studying protein degradation pathways, or examining cellular stress responses, this antibody supports diverse research applications requiring reliable PSMA1 detection in human samples.