PSMA1 Antibody

Datasheet

Code	CSB-PA018865LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate All lanes: PSMA1 antibody at 3.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 31 kDa Observed band size: 30 kDa Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA018865LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA018865LA01HU at dilution of 1:100 Immunofluorescent analysis of PC-3 cells using CSB-PA018865LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunoprecipitating PSMA1 in K562 whole cell lysate Lane 1: Rabbit control IgG (1μg) instead of CSB-PA018865LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000) Lane 2: CSB-PA018865LA01HU (8μg) + K562 whole cell lysate (500μg) Lane 3: K562 whole cell lysate (10μg)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PSMA1 Polyclonal antibody

Uniprot No.

P25786

Target Names

PSMA1

Alternative Names

30 kDa prosomal protein antibody; HC 2 antibody; HC2 antibody; Macropain subunit C2 antibody; Macropain subunit nu antibody; MGC14542 antibody; MGC14575 antibody; MGC14751 antibody; MGC1667 antibody; MGC21459 antibody; MGC22853 antibody; MGC23915 antibody; Multicatalytic endopeptidase complex subunit C2 antibody; NU antibody; PROS 30 antibody; PROS-30 antibody; PROS30 antibody; Proteasome (prosome macropain) subunit alpha type 1 antibody; Proteasome alpha 1 subunit antibody; Proteasome component C2 antibody; Proteasome nu chain antibody; Proteasome subunit alpha type 1 antibody; Proteasome subunit alpha type I antibody; Proteasome subunit alpha type-1 antibody; Proteasome subunit nu antibody; Protein P30 33K antibody; PSA1_HUMAN antibody; PSC 2 antibody; PSC2 antibody; PSMA 1 antibody; psmA1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Proteasome subunit alpha type-1 protein (163-263AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PSMA1 Antibody (Product code: CSB-PA018865LA01HU) is Non-conjugated. For PSMA1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018865LB01HU	PSMA1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA018865LC01HU	PSMA1 Antibody, FITC conjugated
Biotin	CSB-PA018865LD01HU	PSMA1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

Gene References into Functions

In thyroid hemiagenesis, genomic examinations revealed the presence of four recurrent defects (three deletions and one duplication) affecting highly conservative proteasome genes PSMA1, PSMA3, and PSMD3. PMID: 28390009
Data indicate that RNA interference screens identified PSMA1, a component of the 20S proteasome as target of non-small cell lung cancer (NSCLC) cytotoxicity. PMID: 24040035
LC/MS/MS analysis revealed that the C-terminal 16 residues of sperm alpha6 subunit are processed. PMID: 21703233

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peptidase T1A family

Database Links

HGNC: 9530

OMIM: 602854

KEGG: hsa:5682

STRING: 9606.ENSP00000414359

UniGene: Hs.102798

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Monoclonal Antibody Drugs Overview Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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