PSMD14 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA886270A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), U251 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Jurkat whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse stomach tissue lysate(30µg), Rat stomach tissue lysate(30µg) All lanes:PSMD14 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time：1min IHC image of CSB-RA886270A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of PC-3 cell with CSB-RA886270A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA886270A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

O00487

Target Names

PSMD14

Alternative Names

26S proteasome non-ATPase regulatory subunit 14 antibody; 26S proteasome regulatory subunit rpn11 antibody; 26S proteasome-associated PAD1 homolog 1 antibody; 26S proteasome-associated PAD1 homolog antibody; PAD1 antibody; PAD1, yeast, homolog of antibody; POH1 antibody; Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody; Proteasome 26S subunit non ATPase 14 antibody; PSDE_HUMAN antibody; Psmd14 antibody; RPN11 antibody; Testis tissue sperm binding protein Li 69n antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PSMD14 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25G9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PSMD14, also known as POH1 or RPN11, serves as an essential deubiquitinating enzyme within the 19S regulatory particle of the 26S proteasome. This metalloprotease cleaves ubiquitin chains from substrates prior to their degradation, making it a critical regulator of protein homeostasis. Beyond its canonical proteasomal function, PSMD14 has emerged as a significant player in DNA damage repair, cell cycle progression, and has been implicated in various malignancies, positioning it as a valuable target for researchers investigating proteostasis and cancer biology.

This recombinant monoclonal antibody, generated from a defined rabbit clone (25G9) against a synthetic peptide derived from human PSMD14, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is fixed, researchers can expect uniform performance across different lots, eliminating a common source of experimental variability that can complicate long-term studies.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects a clean 35 kDa band matching the predicted molecular weight across diverse human cell lines including MCF7, K562, U251, SH-SY5Y, Jurkat, and COLO205, with cross-species reactivity confirmed in mouse and rat stomach tissue lysates. Immunohistochemistry staining has been optimized in human liver cancer tissue sections, while immunofluorescence studies in PC-3 cells reveal the expected subcellular localization pattern. Flow cytometry validation in 786-O cells further extends the application flexibility available to researchers.

Whether investigating proteasome function, exploring ubiquitin-dependent pathways, or examining PSMD14's emerging roles in oncogenesis, this antibody provides a reliable tool for cell biology research across multiple experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.

Gene References into Functions

overexpression of RPN11 in breast cancer tissues was associated with an advanced clinical stage. Patients with tumors with high expression of RPN11 had worse prognosis. PMID: 28535005
Deubiquitylase POH1 stabilizes E2F1 protein through binding to and deubiquitylating E2F1 in liver cancer. PMID: 26510456
The data demonstrated that proteasomal POH1 is a key de-ubiquitinating enzyme that regulates ubiquitin conjugates generated in response to damage and that several aspects of the DNA double-strand break response are regulated by the proteasome. PMID: 22909820
Disorder of POH1 expression is involved in the onset of idiopathic nephrotic syndrome (INS), and confers multidrug resistance in children with INS PMID: 19419512
Down-regulation of PSMD14 results in decreased cell proliferation, cell cycle arrest and senescence. A comparative study with PSMB5, revealed that PSMB5 and PSMD14 have different effects on cell cycle, senescence and associated molecular events. PMID: 19732767
Ectopic expression of POH1 in HEK293 cells decreased the level of c-Jun ubiquitination, leading to significant accumulation of the protein and a corresponding increase in AP1-mediated gene expression PMID: 16569633
An intact zinc metalloproteinase motif of Poh1 is essential for cell viability and 26S proteasome function. PMID: 17237285
Specificity for K63-linked polyubiquitin is a common property of the JAMM/MPN+ family of deubiquitinating enzymes. PMID: 19214193

Hide All

Protein Families

Peptidase M67A family, PSMD14 subfamily

Tissue Specificity

Widely expressed. Highest levels in heart and skeletal muscle.

Database Links

HGNC: 16889

OMIM: 607173

KEGG: hsa:10213

STRING: 9606.ENSP00000386541

UniGene: Hs.740477

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address