Product Details

Uniprot No.

P15151

Target Names

PVR

Alternative Names

Poliovirus receptor (Nectin-like protein 5) (NECL-5) (CD antigen CD155), PVR, PVS

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human PVR

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19C12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PVR, also known as CD155 or Nectin-like protein 5, serves as a critical immunoregulatory molecule that has gained substantial attention in cancer immunology and infectious disease research. Originally identified as the cellular receptor for poliovirus entry, PVR has since emerged as a key player in tumor immune evasion through its interactions with TIGIT and CD96 on immune cells, making it a compelling target for checkpoint immunotherapy studies.

This recombinant monoclonal antibody, generated from clone 19C12, offers researchers the reproducibility and consistency that sequence-defined production provides. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that each lot delivers identical performance characteristics, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host origin combined with monoclonal specificity yields high-affinity binding to human PVR, developed against a synthetic peptide immunogen.

Validation studies confirm reliable performance across multiple experimental platforms. Western blot analysis of A549 whole cell lysate demonstrates clean detection at the predicted 70 kDa molecular weight, with optimal results achieved at dilutions between 1:500 and 1:2000. This lung adenocarcinoma cell line represents a well-characterized model for PVR expression studies. Immunohistochemistry validation in paraffin-embedded human lung cancer tissue, performed at 1:50 to 1:200 dilutions with citrate buffer antigen retrieval, reveals specific staining patterns suitable for tissue-based investigations.

The affinity-purified antibody arrives in a glycerol-containing buffer optimized for long-term storage stability. For researchers investigating viral pathogenesis, tumor microenvironment dynamics, or immune checkpoint biology, this antibody provides a dependable tool for characterizing PVR expression across cellular and tissue contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.; (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.; (Microbial infection) Acts as a receptor for Pseudorabies virus.; (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

Gene References into Functions

These findings highlight the importance of the TIGIT/CD226/PVR axis as an immune checkpoint barrier that could hinder future "cure" strategies requiring potent HIV-specific CD8(+) T cells PMID: 28084312
Study investigated the more detailed mechanism for this cis-interaction of Necl-5 with the PDGF receptor beta. Necl-5 contains three Ig-like domains and the PDGF receptor beta contains five Ig-like domains at their extracellular regions; showed that the third Ig-like domain of Necl-5 cis-interacted with the fifth Ig-like domain of the PDGF receptor beta. PMID: 29431243
Studied association of poliovirus receptor (PVR/CD155) mutation and cleft lip and cleft palate. Validated previous findings that PVR/CD155 markers are associated with cleft lip and palate. PMID: 29381645
The authors demonstrate that HIV and specifically Nef and/or Vpu do not modulate CD155 on infected primary T cells and both CD155 and NKG2D ligands synergize as a natural killer cell receptor to trigger natural killer cell lysis of the infected cell. PMID: 27296670
Data show that gastric cancer cells inhibit T-cell metabolism through CD155/TIGIT signaling. PMID: 28883004
Studies showed that CD155 was frequently overexpressed in human malignant tumors. Its overexpression promotes tumor cell invasion and migration, and is associated with tumor progression. [review] PMID: 28730595
soluble CD226 elevated in sera of CTCL patients would be important for tumor immunity by interacting with CD155 on tumor cells. PMID: 28395975
data reveal that MICA and PVR are directly regulated by human cytomegalovirus immediate early proteins, and this may be crucial for the onset of an early host antiviral response PMID: 27733551
The SNP detection assay was successfully developed for identification of Ala67Thr polymorphism in human PVR/CD155 gene. The SNP assay will be useful for large scale screening of DNA samples. PMID: 27834324
sCD155 levels were significantly decreased after surgical resection of cancers. Thus, sCD155 level in serum may be potentially useful as a biomarker for cancer development and progression PMID: 27049654
implying that TIGIT exerts immunosuppressive effects by competing with DNAM-1 for the same ligand, CD155 PMID: 26842126
The present study provides evidence that regulation of the PVR/CD155 DNAM-1 ligand expression by nitric oxide may represent an additional immune-mediated mechanism and supports the anti-myeloma activity of nitric oxide donors. PMID: 25609078
Our findings suggest that loss of CD155 expression may play an important role in the immune escape of HCC cells and thus CD155 may serve as a prognostic marker as well as a potential therapeutic target for HCC. PMID: 25320021
CD155 may play a critical role through both immunological and non-immuno logical mechanisms in pancreatic cancer and may be a therapeutic target for this intractable malignancy. PMID: 25862891
CD155 (PVR/Necl5) mediates a costimulatory signal in CD4+ T cells and regulates allergic inflammation. PMID: 25972481
The cell-surface receptor (Pvr) catalyzes a large structural change in the poliovirus that exposes membrane-binding protein chains. PMID: 25631086
In granulosa cells, there are significant changes in expression during follicular maturation. PMID: 24828608
UPR decreases CD226 ligand CD155 expression and sensitivity to NK cell-mediated cytotoxicity in hepatoma cells. PMID: 25209846
Ala residues 10, 14 and 18 in the TM domain of Vpu are required for CD155 downregulation. PMID: 25113908
TIGIT/PVR ligation signaling mediates suppression of IFN-gamma production via the NF-kappaB pathway. PMID: 24817116
UL141 can inhibit cell-surface expression of both natural killer (NK) cell-activating ligand CD155 as well as TRAIL death receptors (TRAIL-R1 and TRAIL-R2). PMID: 24598754
Vpr upregulates PVR during Hiv-1 infection by activating ATR kinase that triggers the DNA damage rsponse pathway and G2 arrest. PMID: 24045107
The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans. PMID: 23980210
PVR resides in the recently identified lateral border recycling compartment, similar to PECAM and CD99. PMID: 23333754
findings suggest Necl-5 expression in lung cancer cells is crucial for their invasiveness in the cancer-stromal interaction PMID: 23276719
The concordant computational and experimental data of the present study indicate that the extent of NECL-5 expression correlates with melanoma progression. PMID: 22929570
we demonstrated the expression of both CD155 mRNA and protein in bone and soft tissue sarcoma cell lines PMID: 22692919
Expression of PVR in B-ALL cells is modulated by epigenetic mechanisms. PMID: 22169283
This investigation has enhanced understanding of cell invasion and confirmed CD44 to play a more significant role in this biological process than CD155. PMID: 22363471
the PVR downmodulation by Nef and Vpu is a strategy evolved by HIV-1 to prevent NK cell-mediated lysis of infected cells. PMID: 22301152
Data show that a high expression of CD112 and CD155 (DNAM-1 ligands) on leukemic blasts. PMID: 21383766
The host TICAM-1 pathway, particularly in macrophages, serves as a source of type I interferon induction that protects poliovirus (PV) receptor-bearing transgenic mice from PV infection and paralytic death. PMID: 21998457
CD155 is an IFNgamma-inducible immune regulatory protein on the surface of human endothelial cells that attenuates the acquisition of effector functions in CD8 T cells. PMID: 21330602
Necl-5 plays a role in mediating tumor cell invasion and that the overexpression of Necl-5 in cancer cells has clinical significance for prognostic evaluation of patients with primary pulmonary adenocarcinoma. PMID: 20331633
We propose that the cytoplasmic domain may target CD155-containing endocytic vesicles to the microtubular network PMID: 11751937
activation of expression of sonic hedgehog protein PMID: 11983699
Data show that both PVR and Nectin-2 represent specific ligands for the DNAM-1 triggering receptor. PMID: 12913096
CD155 may have an important role in cellular function PMID: 12943679
These data indicate that Tage4 represents the functional orthologue of CD155 in mouse. PMID: 14652024
that DNAM-1 regulates monocyte extravasation via its interaction with CD226 expressed at endothelial junctions on normal cells. PMID: 15136589
These results suggest that CD155alpha plays a role in the regulation of cell adhesion and cell motility. PMID: 15194502
cytoplasmic domain of PVR directly interacts with Tctex-1 and plays an important role in retrograde transport of poliovirus-containing vesicles along microtubules in vivo PMID: 15194795
Upregulation of the molecular target CD155 renders explant cultures of high-grade malignant gliomas highly susceptible to a prototype oncolytic poliovirus recombinant. PMID: 15279713
Analysis of the ligands for triggering NK receptors revealed the consistent expression of cd155 and cd112 in myeloid leukemias, and less frequent expression in lymphoblastic leukemias PMID: 15536144
Evasion of NK cell killing was mediated by human cytomegalovirus UL141 blocking surface expression of CD155 PMID: 15640804
Necl-5 has a critical role in integrin alphavbeta3 clustering and focal complex formation PMID: 17446174
Results describe the establishment of a poliovirus oral infection system in human poliovirus receptor-expressing transgenic mice that are deficient in alpha/beta interferon receptor. PMID: 17507470
no statistically significant association between this marker allele and non-syndromic clefting PMID: 17534374
CD155, at least in part, enhances the proliferation of ras-mutated cells PMID: 17893876
crystal structure of C155 D1D2 has been determined to 3.5-A resolution and fitted into approximately 8.5-A resolution cryoelectron microscopy reconstructions of the virus-receptor complexes for the 3 PV serotypes PMID: 19011098

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Subcellular Location

[Isoform Alpha]: Cell membrane; Single-pass type I membrane protein.; [Isoform Delta]: Cell membrane; Single-pass type I membrane protein.; [Isoform Beta]: Secreted.; [Isoform Gamma]: Secreted.

Protein Families

Nectin family

Database Links

HGNC: 9705

OMIM: 173850

KEGG: hsa:5817

STRING: 9606.ENSP00000402060

UniGene: Hs.171844

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Code	CSB-RA567944A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: Poliovirus Receptor antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa IHC image of CSB-RA567944A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.42% DAB.
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PVR Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background