Phospho-MAPK3 (T202/Y204) + MAPK1 (T185/Y187) Recombinant Monoclonal Antibody

The vectors expressing anti-MAPK3/MAPK1 antibody were constructed as follows: immunizing an animal with a synthesized peptide derived from human Phospho-MAPK3 (T202/Y204) + MAPK1 (T185/Y187), isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening MAPK3/MAPK1 antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant MAPK3/MAPK1 antibody. Recombinant MAPK3/MAPK1 antibody in the culture medium was purified using affinity-chromatography. It can react with MAPK3/MAPK1 protein from Human and is used in the ELISA, WB.

The phosphorylated mitogen-activated protein kinase3/1 (MAPK3/1) protein is expressed in some primordial follicles and all growing follicles. Culture of 3 days post-parturition (dpp) ovaries with the MAPK3/1 signaling inhibitor U0126 significantly reduced the number of activated follicles and was accompanied by dramatically reduced granulosa cell proliferation and increased oocyte apoptosis. MAPK3 and its isoforms have been commonly ascribed analogous downstream functions due to their striking similarities. However, it is becoming increasingly clear that these isoforms- particularly MAPK3 and MAPK1 have explicitly different functions. While MAPK1 has a more pronounced role in cell proliferation and developmental processes. MAPK3 does not seem to be required during development and its deficiency may be compensated for by MAPK1. So far, a deficiency of MAPK3 in the CNS has been associated with facilitated learning and long-term memory. MAPK3 (Erk1) is important for the induction of T-cell anergy. Our goal was to determine the influence of MAPK3 on the capacity of DC to arm T-cell responses in autoimmunity.