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| Code | CSB-RA618984A380phHU |
| Size | US$350 |
| Uniprot No. | Q15418 |
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| Immunogen | A synthesized peptide derived from Human Phospho-RPS6KA1 (S380) |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:1000 |
| Relevance | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). |
| Form | Liquid |
| Conjugate | Non-conjugated |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method | Affinity-chromatography |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Alias | Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK-1, RPS6KA1, MAPKAPK1A, RSK1 |
| Immunogen Species | Homo sapiens (Human) |
| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunoprecipitation (IP) Protocol |
| Research Area | Signal Transduction |
| Gene Names | RPS6KA1 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Function | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration |
| Subcellular Location | Nucleus, Cytoplasm |
| Protein Families | Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily |
| Database Links |
HGNC: 10430 OMIM: 601684 KEGG: hsa:6195 STRING: 9606.ENSP00000435412 UniGene: Hs.149957 |
| Pathway |
MAPK signaling pathway
mTOR signaling pathway Neurotrophin signaling pathway |
RPS6KA1 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA980342 | Phospho-RPS6KA1 (Thr348) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA212336 | Phospho-RPS6KA1 (Ser352) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA990220 | RPS6KA1 (Ab-348) Antibody | Human,Mouse,Rat | ELISA,WB,IF |
| CSB-PA906196 | RPS6KA1 (Ab-352) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA240123 | Phospho-RPS6KA1 (Thr573) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA182506 | Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Ser221/227/S218/232) Antibody | Human,Mouse | ELISA,WB |
| CSB-PA698512 | RPS6KA1 (Ab-573) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA004034 | RPS6KA1 Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040251 | Phospho-RPS6KA1 (S380) Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040252 | Phospho-RPS6KA1 (T359/S363) Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040253 | Phospho-RPS6KA1 (T573) Antibody | Human,Mouse,Rat | WB,IHC,IF,ELISA |
| CSB-PA040254 | RPS6KA1 Antibody | Human,Mouse,Rat | WB,IHC,IF,ELISA |
| CSB-PA121989 | RPS6KA1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA798266 | RPS6KA1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-RA618984A359phHU | Phospho-RPS6KA1 (T359+S363) Antibody | Human | ELISA, WB, IF, IP |
| CSB-PA618984LB01HU | RPS6KA1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA618984LD01HU | RPS6KA1 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA618984LA01HU | RPS6KA1 Antibody | Human | ELISA, IF |
| CSB-PA618984LC01HU | RPS6KA1 Antibody, FITC conjugated | Human | ELISA |
RPS6KA1 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP618984HU CSB-EP618984HU CSB-BP618984HU CSB-MP618984HU |
Recombinant Human Ribosomal protein S6 kinase alpha-1(RPS6KA1) | Yeast E.coli Baculovirus Mammalian cell |
2-hydroxyisobutyryl-HIST1H3A (K23) Antibody
Applications: ELISA, WB, IF, ChIP
Species Reactivity: Human
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