Phospho-SMAD5 (S463+S465) Antibody

Code CSB-RA859108A463phHU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • IHC image of CSB-RA859108A463phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Uniprot No. Q99717
Target Names SMAD5
Alternative Names DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; JV5-1 antibody; MAD homolog 5 antibody; MAD, mothers against decapentaplegic homolog 5 antibody; MADH 5 antibody; MADH5 antibody; Mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic, drosophila, homolog of, 5 antibody; Mothers against DPP homolog 5 antibody; MusMLP antibody; SMA and MAD related protein 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD, mothers against DPP homolog 5 antibody; Smad5 antibody; SMAD5_HUMAN antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from Human Phospho-SMAD5 (S463+S465)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 2G11
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Gene References into Functions
  1. We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. We identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. PMID: 29852786
  2. Smad5 acts as a intracellular pH messenger and maintains the bioenergetic homeostasis of cells by regulating cytoplasmic metabolic machinery. PMID: 28675158
  3. Data suggest that differences in expression levels in granulosa-like tumor cells and granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS) are due to increased expression of microRNA-27a-3p in GCs caused by insulin resistance in PCOS; microRNA-27a-3 expression is up-regulated in GCs in PCOS; overexpression of miR-27a-3p inhibits SMAD5 expression and promotes apoptosis. (SMAD5 = SMAD family member 5) PMID: 29029022
  4. Here the involvement of the pathway in adult brain function is suggested. This exploratory study establishes a strategy to better identify neuronal molecular signatures that are potentially associated with mental illness and cognitive deficits. We propose that the SMAD pathway may be a novel target in addressing cognitive deficit of SZ in future studies. PMID: 27727244
  5. miR-23a and miR-27a target SMAD5 and regulate apoptosis in human granulosa cells via the FasL-Fas pathway PMID: 26400397
  6. Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation hBMSCs through regulating Smad5-RUNX2 signaling axis. PMID: 26809090
  7. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. PMID: 25754204
  8. among the 15 SNPs, rs3206634 was significantly associated with KD in a recessive model (odds ratio = 2.31, p = 0.019), whereas there was no association between any of the 15 SNPs and CALs. PMID: 24163009
  9. Specific gene siRNAs knock-down further confirmed the osteogenic effects of Genistein on BMP2, SMAD5 and RUNX2 protein expression PMID: 24339730
  10. ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad5 PMID: 23384547
  11. found that restoration of SMAD5, in addition to the TGF-beta type II receptor, which was epigenetically silenced by the latent viral protein latency-associated nuclear antigen, sensitized BC3 cells to the cytostatic effect of TGF-beta signaling PMID: 22013049
  12. Studies indicate SMAD5beta isoform plays role in hematopoietic stem cell homeostasis. PMID: 20148926
  13. regulatory signals are active at transcriptionally subnuclear sites PMID: 12060751
  14. Smad5 activation by BMP4 in human hematopoietic cells results in significantly increased proliferation of erythroid progenitors and formation of glycophorin-A+ cells. PMID: 12064918
  15. up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection PMID: 12473652
  16. Results demonstrate a mitochondrial distribution of Smad5 in non-stimulated chondroprogenitor cells. PMID: 12849988
  17. SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma PMID: 14670176
  18. In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad5. PMID: 15877825
  19. We used homology-modeling techniques to generate a reliable molecular model of the Smad5 MH1 domain based on the crystal structure of Smad3 MH1 domain PMID: 16243555
  20. SMAD5 gene was found to be associated with schizophrenia. PMID: 18298822

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Subcellular Location Cytoplasm, Nucleus
Protein Families Dwarfin/SMAD family
Tissue Specificity Ubiquitous.
Database Links

HGNC: 6771

OMIM: 603110

KEGG: hsa:4090

STRING: 9606.ENSP00000441954

UniGene: Hs.167700

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