Code | CSB-RA859108A465phHU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
In the production of the phospho-SMAD5 (S463/S465) recombinant monoclonal antibody, the initial phase commences with the extraction of genes responsible for encoding this SMAD5 antibody. These genes are acquired from rabbits that have been previously exposed to a synthesized peptide originating from the human SMAD5 protein phosphorylated at S463/S465. Subsequently, these antibody genes are seamlessly integrated into specialized expression vectors. Following this genetic modification, the vectors are introduced into host suspension cells, which are then meticulously cultivated to encourage the robust expression and secretion of antibodies. Following this cell culture phase, the phospho-SMAD5 (S463/S465) recombinant monoclonal antibody is subjected to a thorough purification process utilizing affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Ultimately, the antibody's functionality is comprehensively evaluated through a battery of assays, including ELISA and WB tests, conclusively confirming its capacity to interact effectively with the human SMAD5 protein phosphorylated at S463/S465.
Phosphorylation of SMAD5 at S463 and S465 is a fundamental mechanism for regulating BMP and TGF-β signaling pathways, controlling gene expression, and coordinating various cellular processes during development and tissue homeostasis. Dysregulation of this phosphorylation event can have significant implications in developmental disorders and cancer.
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