SEC62 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA169564A0HU
Size	US$210
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Image	IHC image of CSB-RA169564A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA169564A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA169564A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q99442

Target Names

SEC62

Alternative Names

SEC62; TLOC1; Translocation protein SEC62; Translocation protein 1; TP-1; hTP-1

Species Reactivity

Human

Immunogen

A synthesized peptide from human SEC62 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SEC62 is a critical component of the Sec61 translocon complex, playing an essential role in the post-translational translocation of proteins across the endoplasmic reticulum membrane. This protein has garnered significant research attention due to its involvement in cellular stress responses and its emerging role in cancer biology, where SEC62 amplification and overexpression have been linked to tumor progression and metastasis in several malignancies.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human SEC62, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies and collaborative projects.

The antibody has been validated for immunohistochemistry and flow cytometry applications, providing flexibility across different experimental workflows. IHC validation demonstrates clear detection in paraffin-embedded human kidney tissue and human colorectal cancer specimens at 1:100 dilution using citrate buffer antigen retrieval, making it suitable for both normal tissue characterization and oncology research. Flow cytometry validation in MCF-7 breast cancer cells confirms its utility for intracellular detection of SEC62 in fixed and permeabilized samples, with clear separation from isotype control.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody is well-suited for researchers investigating ER stress pathways, protein translocation mechanisms, or the role of SEC62 in cancer development and progression. Its validated performance in human tissues and cancer cell lines makes it a reliable tool for both basic research and translational oncology studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Mediates post-translational transport of precursor polypeptides across endoplasmic reticulum (ER). Proposed to act as a targeting receptor for small presecretory proteins containing short and apolar signal peptides. Targets and properly positions newly synthesized presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen.

Gene References into Functions

Results show higher SEC62 in the lymph node metastases from head and neck squamous cell carcinomas and cervical cancer of unknown primary patients compared with the primary tumor. Tumors from N1 to N3 stage exhibit increasing SEC62 expression in the lymphatic metastases. Its knockdown resulted in tumor cell line migration inhibition while, its overexpression stimulated cell migration. PMID: 28002801
High Sec62 expression is associated with dysplastic cervical lesions. PMID: 27553742
identify Sec62 as a critical molecular component in maintenance and recovery of endoplasmic reticulum homeostasis PMID: 27749824
these studies identify TLOC1 and SKIL as driver genes at 3q26 and more broadly suggest that cooperating genes may be coamplified in other regions with somatic copy number gain. PMID: 23764425
Sec62 overproduction significantly correlates with reduced non-small lung cancer patient survival. PMID: 24304694
Phosphorylation of Sec63 by CK2 enhanced its binding to Sec62 PMID: 23287549
Silencing the SEC62 gene inhibits post-translational transport of small presecretory proteins into endoplasmic reticulum. PMID: 22375059
the Sec62-dependent translocation pathway serves as a fail-safe mechanism to ensure efficient secretion of small proteins and provides cells with an opportunity to regulate secretion of small proteins independent of the signal recognition particle pathway PMID: 22648169
These results revealed that cyclin B1 and Sec62 may be candidate biomarkers and potential therapeutic targets for HBV-related HCC recurrence after surgery. PMID: 22682366
The results indicate that SEC62 represents a potential candidate oncogene in the amplified 3q region in cases of non-small cell lung cancer. PMID: 22197383
Our data indicate a crucial function of Sec62 in the response to thapsigargin-induced endoplasmic reticulum stress. PMID: 21557272
after silencing of SEC62 the cell migration and the invasive potential of the cells was blocked or at least dramatically reduced while cell viability was hardly affected. SEC62 gene may indeed be considered a target gene in the therapy of various tumors PMID: 20669223
The gene TLOC1/SEC62 revealed the highest frequency (50%) of copy number gains in the prostate cancer and was found to be up-regulated at the mRNA level. PMID: 16547154

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

SEC62 family

Database Links

HGNC: 11846

OMIM: 602173

KEGG: hsa:7095

STRING: 9606.ENSP00000337688

UniGene: Hs.744859

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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