Product Details

Uniprot No.

Target Names

SET

Alternative Names

2PP2A antibody; HLA DR associated protein II antibody; HLA-DR-associated protein II antibody; I 2PP2A antibody; I-2PP2A antibody; I2PP2A antibody; IGAAD antibody; Inhibitor of granzyme A activated DNase antibody; Inhibitor of granzyme A-activated DNase antibody; Inhibitor of GzmA-activated DNase antibody; inhibitor-2 of protein phosphatase-2A antibody; IPP2A2 antibody; PHAPII antibody; Phosphatase 2A inhibitor I2PP2A antibody; protein phosphatase type 2A inhibitor antibody; Protein SET antibody; Set antibody; SET nuclear oncogene antibody; SET translocation antibody; SET translocation (myeloid leukemia-associated) antibody; SET_HUMAN antibody; TAF I antibody; TAF IBETA antibody; TAF-I antibody; TAFI antibody; Template activating factor I antibody; Template-activating factor I antibody; Template-Activating Factor-I, chromatin remodelling factor antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human SET

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SET protein serves as a multifunctional regulator with significant roles in chromatin remodeling, transcriptional regulation, and cell cycle control. As a potent inhibitor of protein phosphatase 2A, SET influences numerous cellular signaling pathways, while its function as an inhibitor of granzyme A-activated DNase connects it to apoptotic processes. The protein's involvement in myeloid leukemia translocations and its designation as a nuclear oncogene make it a compelling target for cancer research and studies of cellular transformation.

This recombinant monoclonal antibody, generated from clone 5E6 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments.

Validation testing demonstrates robust performance across multiple applications, providing flexibility for diverse experimental approaches. Western blot analysis detects SET protein in HeLa, COLO205, HEK293, and HL-60 cell lysates at dilutions between 1:500 and 1:2000. The observed band at 39 kDa runs slightly higher than the predicted 33 kDa molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation or glycosylation. Immunohistochemistry staining has been validated in human colorectal cancer and stomach tissue sections using citrate buffer antigen retrieval. Immunofluorescence microscopy reveals clear nuclear localization in HeLa cells, consistent with SET's known biological function. Flow cytometry analysis further confirms reliable detection in permeabilized HeLa cells.

This antibody supports investigations into PP2A signaling, chromatin dynamics, and oncogenic mechanisms across cancer biology and cell signaling research programs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

Gene References into Functions

hsa_circ_0000673 promoted hepatocellular carcinoma malignant behaviors via regulating miR-767-3p/SET pathway. PMID: 29627570
Findings indicate that TCE may induce hepatocyte apoptosis via SET-mediated dephosphorylation and overexpression of nucleolin. PMID: 28402964
Recent studies showed that SET is overexpressed in breast cancers, ovary cancers and other malignancies. The strong correlation between SET expression levels and survival of ovarian cancer patients, and SET-mediated activation of androgen synthesis, strongly indicate a significant role in gynecologic cancers. Also, SET appears to exert its activity through PP2A-dependent as well as PP2A-independent mechanisms. [review] PMID: 28356029
SET overexpression is associated with GC progression. PMID: 28677734
The results demonstrate that NFkB plays an important role in regulation of the human SET gene expression. PMID: 27351675
SET accumulation is implicated in epigenetic modifications, such as DNA hypomethylation and histone hypoacetylation. It is associated with gene expression regulation and chromatin organization, thereby affecting gene expression control, DNA damage repair, and genome instability. PMID: 28460463
Data show that PP32 and SET/TAF-Ibeta proteins block HAT1-mediated H4 acetylation. PMID: 28977641
SEToverexpression promotes morphological and oncogenic cell transformation of an oral keratinocyte cell. PMID: 28636114
Overexpression of SET-Nup214 in HeLa cells leads to the formation of similar nuclear bodies that recruit CRM1, export cargo proteins, and certain nucleoporins and concomitantly affect nuclear protein and poly(A)(+) RNA export. PMID: 27613868
Molecular chaperone SET-assisted eviction of linker histones and Shugoshins is a fundamental step in mammalian mitotic progression. PMID: 28781233
Our findings suggested the oncogenic role of SET and the adverse prognostic value of SET overexpression in hepatocellular carcinoma (HCC). This alteration defines a subgroup of HCC patients who could benefit from SET antagonists, such as EMQA. PMID: 26876205
This review summarizes and discusses the general mechanisms of H3K4 methylation, and how the six main enzymes from the SET/MLL family (responsible for H3K4me1/me2/me3) function in hematopoiesis and in hematologic malignancies. [review] PMID: 27796741
identification of the oncoprotein SET as a major cellular factor whose binding with p53 is dependent on C-terminal domain acetylation status PMID: 27626385
The findings of the present study suggested that the SET gene may contribute to tumorigenesis and may be a potential novel effective therapeutic target for leukemia and other cancer types. PMID: 27035430
Data indicate that SET protein as a direct target of microRNA miR-125b, and the downregulation of SET, observed during tumor migration, was affected by the overexpression of miR125b. PMID: 27383536
significance of SET/I2PP2A-mediated PP2A and non-PP2A pathways in polycystic ovary syndrome PMID: 27836688
Both SET protein levels (and BACE1) were increased in Down syndrome patients. PMID: 24935721
SET interacts with the Kruppel-associated box (KRAB)-associated co-repressor KAP1, and its overexpression results in the sustained retention of KAP1 and Heterochromatin protein 1 (HP1) on chromatin PMID: 25818296
the results of the present study demonstrated that SET was associated with paclitaxel resistance in MCF-7/PTX cells, and that paeonol reversed paclitaxel resistance in MCF-7/PTX cells by downregulating the activity of the SET/PP2A/Akt pathway. PMID: 25760096
These findings establish a framework for understanding the molecular basis of cytochrome c-mediated blocking of SET/TAF-Ibeta. PMID: 26216969
Low SET expression is associated with bone metastasis in renal cell carcinoma. PMID: 26115722
SET overexpression is a frequent event in metastatic colorectal cancer that plays a potential oncogenic role associated with worse outcome and resistance to oxaliplatin. PMID: 25388166
A functional link between SET-mediated NDRG1 regulation. PMID: 25152373
Therefore, SET overexpression in HEK293 cells promotes mitochondrial fission and reduces autophagic flux in apparent association with up-regulation of UCP2 and UCP3. PMID: 25656576
The stable suppression of SET through RNA interference inhibited the growth, migration and invasion of breast cancer cells. Knockdown of SET increased the activity and expression of PP2Ac and decreased the expression of matrix metalloproteinase 9. PMID: 25234598
Data indicate a therapeutic paradigm of inhibitor-2 of protein phosphatase-2A (SET) antagonism using OP449 in combination with tyrosine kinase inhibitors for the treatment of chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) cell lines. PMID: 24436473
Inactivation of nuclear localization signal causes retention of I2PP2A in the cell cytoplasm, where it promotes Tau hyperphosphorylation by affecting PP2A signaling. PMID: 25128526
SET/TAF-Ibeta inhibits FoxO1 acetylation and activates its transcriptional activity toward p21 PMID: 24983498
SET accumulation has important actions in head and neck squamous cell carcinoma PMID: 24555657
SET/TAF-Ibeta interacts with Ku70/80 in the nucleus and inhibits Ku70 acetylation. PMID: 24305947
SET is overexpressed in about 50-60% and CIP2A in about 90% of breast cancers. PMID: 24927563
Set-beta differentially regulates axon growth and regeneration depending on subcellular localization and phosphorylation. PMID: 24849368
C6-ceramide inhibits I2PP2A mediated upregulation of c-Myc and downregulation of histone acetylation in prostate cancer cells. PMID: 24025258
When compared with SET-NUP214-negative patients, SET-NUP214-positive patients showed a significantly higher rate of corticosteroid resistance (91% vs 44%; P = .003) and chemotherapy resistance (100% vs 44%; P = .0001). PMID: 24449214
Data show that SET accumulation up-regulated hnRNPK mRNA and total/phosphorylated protein, promoted hnRNPK nuclear location, and reduced Bcl-x mRNA levels. PMID: 24508256
Detention of cytoplasmic SET protein occurs with Ser9 phosphorylation in Alzheimer's disease. PMID: 23374587
TAF-Ibeta promotes BZLF1 expression and subsequent lytic infection by affecting chromatin at the BZLF1 promoter PMID: 23691099
Recombinant SET with phosphorylation-mimic Ser171 to Glu substitution reduced its inhibitory effects on PP2A phosphatase activity compared with Ser171 to Ala substituted or wild-type SET PMID: 23251465
SET is potential marker for head and neck squamous cell carcinoma associated with cancer cell resistance PMID: 22739068
SET protein interacts with intracellular domains of the gonadotropin-releasing hormone receptor and differentially regulates receptor signaling to cAMP and calcium in gonadotrope cells PMID: 23233674
A SET mutant, lacking a nuclear localization signal, SET(DeltaNLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. PMID: 23195690
SET downregulated repair genes ATM, BRCA1 and CHEK2 and upregulated TP53 PMID: 23106910
SET/TAF-Ibeta is sequentially recruited to the target gene promoter ATF3 after the PRC2 complex via H3K27me recognition and may offer additive effects in the repression of the target gene. PMID: 22796192
SET knockdown increased high-affinity binding of agonist in intact cells and membrane preparations. PMID: 22466417
Results showed that SET is significantly overexpressed in pediatric acute lymphoblastic leukemia samples, and an increased level of SET might contribute to leukemic process. PMID: 22677993
SET overexpression is a key mechanism in the inhibition of PP2A in acute myeloid leukemia. PMID: 22133779
Suggest that accumulated SET could act via Akt/PTEN either as cell survival signal or as oxidative stress sensor for cell death. PMID: 22143534
Data describe the relatively high incidence of SET-NUP214 rearrangement in adult T-ALLs, and demonstrate comprehensive clinical, phenotypic, and genetic characteristics of this entity. PMID: 21720744
crystals of template-activating factor Ibeta diffracted to 2.7 A resolution and belonged to space group P4(3)2(1)2. PMID: 20693670
TAF-I is a key molecule that regulates linker histone-mediated chromatin assembly and disassembly PMID: 21940793

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Involvement in disease

A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Subcellular Location

Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus, nucleoplasm. Note=In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium.

Protein Families

Nucleosome assembly protein (NAP) family

Tissue Specificity

Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

Database Links

HGNC: 10760

OMIM: 600960

KEGG: hsa:6418

STRING: 9606.ENSP00000361777

UniGene: Hs.436687

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Code	CSB-RA561645A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HeLa whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HEK293 whole cell lysate, HL-60 whole cell lysate(30µg) All lanes: SET antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 39 kDa Exposure time: 2min IHC image of CSB-RA561645A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA561645A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA561645A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA561645A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SET Recombinant Monoclonal Antibody

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