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SIPA1 Recombinant Monoclonal Antibody

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Datasheet
Code CSB-RA822213MA1HU
Size $49.9
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Image
  • Immunofluorescence staining of Hela cell with CSB-RA822213MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
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Product Details

Uniprot No.
Q96FS4
Target Names
SIPA1
Alternative Names
GTPase activating protein Spa 1 antibody; GTPase-activating protein Spa-1 antibody; MGC102688 antibody; MGC17037 antibody; p130 SPA-1 antibody; p130 SPA1 antibody; Signal induced proliferation associated 1 antibody; Signal induced proliferation associated gene 1 antibody; Signal induced proliferation associated protein 1 antibody; Signal-induced proliferation-associated protein 1 antibody; SIPA 1 antibody; Sipa-1 antibody; Sipa1 antibody; SIPA1_HUMAN antibody; Spa1 antibody
Species Reactivity
Human
Immunogen
Recombinant Human SIPA1 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
20H11
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Protocols
ELISA Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

SIPA1, also known as signal-induced proliferation-associated protein 1 or GTPase-activating protein Spa-1, functions as a critical regulator of Rap1 GTPase signaling. By accelerating the hydrolysis of GTP-bound Rap1 to its inactive GDP-bound state, SIPA1 influences fundamental cellular processes including adhesion, proliferation, and migration. Dysregulation of SIPA1 has been implicated in cancer metastasis and immune cell function, making it a compelling target for researchers investigating cell signaling networks and disease mechanisms.

This recombinant monoclonal antibody, clone 20H11, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The human IgG1 isotype format, generated against recombinant human SIPA1 protein, ensures reliable target recognition in human samples.

Validation through immunofluorescence demonstrates clear cytoplasmic localization in HeLa cells, with recommended dilutions ranging from 1:50 to 1:200 providing flexibility to optimize signal intensity for your specific imaging setup. The validation protocol employed standard fixation with 4% formaldehyde and 0.2% Triton X-100 permeabilization, offering a straightforward starting point for your experiments. Additional compatibility with ELISA extends the utility of this antibody across detection platforms.

Supplied in a liquid format with glycerol-based buffer for stability, this affinity-purified antibody is ready for immediate use in cell biology research exploring Rap1-mediated signaling, cellular adhesion dynamics, or proliferation control mechanisms. Whether investigating SIPA1's role in normal physiology or its contribution to pathological processes, this antibody provides the molecular specificity your research demands.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state. Affects cell cycle progression.
Gene References into Functions
  1. SIPA1 promotes oral squamous cell carcinoma metastasis by regulating the ITGB1 and MMP7. PMID: 28237246
  2. Study shows that SIPA1 mRNA and protein expression are down-regulated in gastric cancer cells and correlate with tumor grading, invasion and lymph node metastasis as well as higher expression of VEGFA. Low SIPA1 levels in gastric cancer may then accelerate tumor development and progression by promoting VEGFA expression to increase vascular density. PMID: 28362978
  3. results suggest that SIPA1 and RRP1B germline polymorphisms are important for breast cancer prognosis PMID: 26901824
  4. Our results indicate, for the first time, that the SIPA1 -313A>G may have a prognostic role in unresected NSCLC making it a potential predictor of poor survival due to earlier progression. PMID: 25352027
  5. nuclear SIPA1 contributes to breast cancer cell invasion through the regulation of integrin beta1 signaling. PMID: 24704834
  6. BRD4 short isoform interacts with RRP1B, SIPA1 and components of the LINC complex at the inner face of the nuclear membrane. PMID: 24260471
  7. This meta-analysis suggests that rs746429 is associated with the risk of breast cancer. PMID: 24006220
  8. SIPA1 SNPs, rs746429 and rs2306364, were associated with decreased risk of triple-negative breast tumors. PMID: 23771732
  9. Polymorphism in Sipa1 promoter gene is associated with lung cancer. PMID: 23661532
  10. Patients with metastatic breast cancer with SIPA1 545 T/T genotype had a significantly worse overall survival than did patients with C/T or C/C genotype (50.0% vs. 62.9%, P = 0.042). PMID: 23358895
  11. SIPA1 expression is increased in human colorectal cancer. PMID: 22990111
  12. In this case-control study, SNPs in SIPA1 varied statistically in cervical cancer patients with and without nodal metastases and in MMP9 after controlling for stage and lymphvascular space invasion. PMID: 19906411
  13. Data identif a Rap GTPase-activating protein, signal-induced proliferation-associated protein 1 (SPA-1), as a factor that interacts with Brd4. PMID: 15456879
  14. SIPA1 germline polymorphisms are associated with aggressive disease behavior in breast cancer. PMID: 16563182
  15. SPA1 regulates the maintenance and differentiation of embryonic stem cells. PMID: 18033671
  16. it is unlikely that SIPA1 plays a pathogenetic role in the development of juvenile myelomonocytic leukemia PMID: 18492118
  17. Our results do not support a relationship between SIPA1 polymorphisms and breast cancer risk or subsequent survival PMID: 19089925
  18. SIPA1 SNP rs3741378 was associated with increased breast cancer incidence. PMID: 19765277

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Subcellular Location
Nucleus. Cytoplasm, perinuclear region. Endomembrane system; Peripheral membrane protein.
Tissue Specificity
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Database Links

HGNC: 10885

OMIM: 602180

KEGG: hsa:6494

STRING: 9606.ENSP00000377771

UniGene: Hs.530477

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Related pathways
Leukocyte transendothelial migration Rap1 signaling pathway
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