Product Details

Uniprot No.

Target Names

SRSF3

Alternative Names

Serine/arginine-rich splicing factor 3 (Pre-mRNA-splicing factor SRP20) (Splicing factor, arginine/serine-rich 3), SRSF3, SFRS3 SRP20

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human SRSF3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SRSF3, also known as SRP20, is a member of the serine/arginine-rich splicing factor family that plays essential roles in constitutive and alternative pre-mRNA splicing. This protein regulates splice site selection and participates in mRNA export from the nucleus, making it a critical factor in post-transcriptional gene regulation. SRSF3 has garnered significant research attention due to its involvement in cell proliferation, tumorigenesis, and developmental processes, with altered expression patterns observed across multiple cancer types.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human SRSF3, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation data demonstrates robust performance across multiple applications. Western blot analysis at 1:1000 dilution detects a clean band at the predicted 19 kDa molecular weight in both human cell lines including HT-29, HeLa, and K562, as well as mouse NIH/3T3 cells, confirming cross-species reactivity between human and mouse samples. Immunohistochemistry staining has been successfully performed on paraffin-embedded human lung cancer and testis tissues using citrate buffer antigen retrieval. For cellular localization studies, immunofluorescence analysis in A431 cells and flow cytometry with Jurkat cells provide validated protocols for examining SRSF3 expression at the single-cell level.

This antibody serves researchers investigating RNA processing mechanisms, splicing regulation, and the growing field of splicing factor dysregulation in cancer biology and developmental disorders.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.

Gene References into Functions

Results show that SRSF3 impairs the autoregulation mechanism of SRSF5 and promotes the overexpression of SRSF5 in oral squamous cell carcinoma. PMID: 29857020
TDP43 alters most splicing events with splicing factor SRSF3 in triple-negative breast cancer. PMID: 29581274
Loss of SRSF3 expression was significantly associated with poor survival and shorter disease-free survival, particularly in early stages, in colorectal cancer. PMID: 29131639
Findings reveal the molecular mechanism of SRSF3 in pri-miRNA processing and support the previously proposed explanation for the highly conserved position of CNNC in SRSF3-enhanced pri-miRNA processing. PMID: 29615481
High expression of SRSF3 is associated with oral squamous cell carcinoma. PMID: 27808105
MicroRNA-1908-5p contributes to the oncogenic function of the splicing factor SRSF3 via NKIRAS2 axis in osteosarcoma cells. PMID: 28039456
Dehydroepiandrosterone (DHEA) and cortisol modulate SRSF9 and SRSF3 in a different way and data suggest that the anti-glucocorticoid effect of DHEA, among other mechanisms, is also exerted by modulating the expression of proteins involved in the splicing of the GR pre-mRNA. PMID: 28373129
that SRSF3 controls the expression of the miR-132/212 cluster through regulating REST expression PMID: 28624413
Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons controlled by SRF3. PMID: 27566151
Introduction of point mutations in the SRSF3-binding site or knockdown of SRSF3 expression in cells reduces human papillomavirus 18 929;3434 splicing and E1;E4 production but activates other, minor 929;3465 and 929;3506 splicing. Knockdown of SRSF3 expression also enhances the expression of E2 and L1 mRNAs. PMID: 27489271
data indicate that the Human Papillomavirus E2 protein links the viral replication cycle to epithelial differentiation via SRSF3, a key cellular regulator of high-risk (HR)-HPV gene expression PMID: 26962216
Results suggested that SRSF3 is associated with the initiation and development of OSCC and may be a biomarker and therapeutic target of OSCC. PMID: 27429590
we also demonstrated frequent co-overexpression and positive correlation of DARPP-32, SRp20 and CD44E expression levels in human gastric primary tumors. PMID: 26119931
Results show that YTHDC1 promotes exon inclusion in targeted mRNAs through recruiting pre-mRNA splicing factor SRSF3 while blocking SRSF10 mRNA binding. PMID: 26876937
These data indicate that SRSF3 affects a global change of gene expression to maintain cell homeostasis. PMID: 26704980
We propose that SRSF3 could act as a coordinator of the expression of PDCD4 protein via two mechanisms on two alternatively spliced mRNA isoforms. PMID: 26773498
This new function of SRSF3 not only explains why overexpression of SRSF3 is required for ovarian cancer cell growth and survival but also offers a new insight into the mechanism of the neoplastic transformation. PMID: 26282282
SRSF3 and hnRNP H1 are the first splicing factors identified which regulate the production of these functionally distinct HER2 splice variants and therefore maybe important for the regulation of HER2 signaling. PMID: 26367347
expression either decreased or the protein mislocalized in hepatocellular carcinoma PMID: 25132062
Results identified modulation of SRSF3 activity as the molecular mechanism by which ORF57 promotes RNA splicing. PMID: 25234929
Data suggest that the oncogenic potential of SRSF3 might be realized, in part, through the translational repression of PDCD4 mRNA. PMID: 24292556
Stress-inducible truncated SRSF3 may participate in the acceleration of cell growth through facilitating c-Jun-mediated G1 progression under stressful conditions. PMID: 24190040
This is the first study that demonstrates that rs1122608 confers protection against ischemic stroke and implicates splicing factor SFSR3 in the disease process PMID: 24190014
findings disclose an important role of SRSF3 in the regulation of the G1-to-S-phase progression and alternative splicing of HIPK2 in tumor growth PMID: 23503458
truncated SRSF3 protein may function as a positive regulator of oxidative stress-initiated inflammatory responses in colon cancer cells. PMID: 24284797
Therefore we conclude that SRSF3 promotes exon 9 skipping of caspase-2 pre-mRNA by interacting with exon 8 PMID: 24321384
Work described here examined the punctate pattern of SRp20 localization in the cytoplasm of poliovirus-infected cells, demonstrating the partial co-localization of SRp20 with the stress granule marker protein TIA-1. PMID: 23830997
Digoxin-mediated repression of SRSF3 expression plays a role in the digoxin-mediated inclusion of exon 20 in the IKBKAP transcript generated from the familial dysautonomia mutant allele. PMID: 23711097
downregulation of SRSF3 represents an endogenous mechanism for cellular senescence that directly regulates the TP53 alternative splicing to generate p53beta PMID: 22777358
results further define the mechanism of SRp20 cellular redistribution during picornavirus infections PMID: 23255796
Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver PMID: 23233666
This study does not confirm a role for genetic variants in the SFRS3 and FKBP4 genes in the pathogenesis of corticosteroid-induced ocular hypertension. PMID: 22921020
This study found that significant changes in serine/arginine protein 20 (Srp20) gene expression in AD cases and confirmed this using a second cohort of control/AD. PMID: 22788679
Protein extracts of colon cancer CD133+ cell stem cells were compared to protein extracts of colon cancer cell CD133- stem cells by 2D DIGE. Demonstrated a direct cause-effect relationship between Wnt pathway activation & increased SRp20 expression. PMID: 22623141
Relative levels of SRp20, SRp30c, and SRp40 in TM cells control differential expression of the two alternatively spliced isoforms of the GR and thereby regulate GC responsiveness. PMID: 22205602
a model in which SRp20 interacts with PCBP2 bound to the viral RNA, and this interaction functions to recruit ribosomes to the viral RNA in a direct or indirect manner, with the participation of additional protein-protein or protein-RNA interactions. PMID: 21779168
Silencing of SFRS3 increased IL-1beta secretion due to elevation of IL-1beta and caspase-1 mRNA in addition to active caspase-1 levels. PMID: 21611201
expression of PTB and SRp20 is associated with malignancy of ovarian tumors but not with stage of invasive epithelial ovarian cancer PMID: 20856201
Results suggest that increased SRp20 expression in tumor cells is a critical step for tumor initiation, progression, and maintenance. PMID: 21179588
Epstein-Barr Virus SM protein utilizes cellular splicing factor SRp20 to mediate alternative splicing. PMID: 20810723
The authors report that siRNA knockdown of SRp20 or 9G8 resulted in about a 10 fold decrease in herpes simplex virus 1 yields and in nuclear accumulation of polyA+ RNA. PMID: 20227104
insulin induces the proteasome-dependent degradation of SRp20 as well as the subnuclear relocalization of CLIC1 PMID: 15827065
These results suggest that the C-terminal domain of RPB1 promotes exon skipping by recruiting SRp20 and that this contributes independently of elongation to the transcriptional control of alternative splicing. PMID: 17028590
nucleo-cytoplasmic SR protein, SRp20, functions in internal ribosome entry site (IRES)-mediated translation of a viral RNA. PMID: 17183366
abundant SRp20 in cancer cells or undifferentiated keratinocytes is important for the expression of the viral early E6 and E7 by promoting the expression of cellular transcription factor SP1 for transactivation of viral early promoters. PMID: 18945760
Study reports the splicing factor SRp20 as a novel target gene of beta-catenin/TCF4 signaling. PMID: 18952824
SRp20, SF2/ASF, and CUG-BP1 act antagonistically to regulate IR alternative splicing in vivo and that the relative ratios of SRp20 and SF2/ASF to CUG-BP1 in different cells determine the degree of exon inclusion. PMID: 19047369
SRp20 associate with interphase chromatin, and is released from hyperphosphorylated mitotic chromosomes PMID: 19250906

Hide All

Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

Splicing factor SR family

Database Links

HGNC: 10785

OMIM: 603364

KEGG: hsa:6428

STRING: 9606.ENSP00000362820

UniGene: Hs.405144

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA932304A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: SRSF3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time：2min IHC image of CSB-RA932304A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA932304A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA932304A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA932304A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

SRSF3 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background