Product Details

Uniprot No.

Target Names

Alternative Names

EPB72 like protein 2 antibody; EPB72-like protein 2 antibody; HSPC108 antibody; OTTHUMP00000021320 antibody; Paraprotein target 7 antibody; Paratarg 7 antibody; SLP 2 antibody; SLP-2 antibody; SLP2 antibody; STML2_HUMAN antibody; Stomatin (EPB72) like 2 antibody; Stomatin like 2 antibody; Stomatin like protein 2 antibody; Stomatin like protein 2, mitochondrial antibody; Stomatin-like protein 2 antibody; STOML2 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human STOML2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4B4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

STOML2, also known as stomatin-like protein 2, is a mitochondrial membrane protein that plays a critical role in mitochondrial biogenesis and respiratory chain assembly. Its involvement in cellular energy metabolism and stress responses has made it a protein of significant interest in cancer research, where altered mitochondrial function frequently contributes to tumor progression and metabolic reprogramming.

This recombinant monoclonal antibody, clone 4B4, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and production occurs in controlled expression systems, you can expect consistent performance across experiments and between lot numbers, eliminating a common source of variability in long-term studies.

Validation data demonstrates robust performance across multiple experimental platforms. In Western blot applications, the antibody detects a clean band at the predicted 39 kDa molecular weight across an extensive panel of human cell lines including HeLa, HepG2, COLO-205, A431, A549, SH-SY5Y, and HEK293T. Cross-species reactivity extends to mouse and rat, confirmed through successful detection in brain tissue lysates from both species, making this antibody suitable for comparative studies and work with rodent disease models.

For tissue-based investigations, immunohistochemistry validation shows clear staining in paraffin-embedded human kidney tissue and lung cancer specimens using citrate buffer antigen retrieval. The recommended dilution range of 1:50 to 1:200 for IHC provides flexibility for optimization across different tissue types and fixation conditions.

Whether investigating mitochondrial dysfunction in cancer metabolism or exploring STOML2's emerging roles in cellular stress pathways, this antibody provides a reliable tool for protein detection and localization studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation.

Gene References into Functions

TROP-2, SLP-2 and CD56 were effective diagnostic markers for PTC, especially when they were combined to use. PMID: 29951933
Data show that SLP-2 is upregulated by high cisplatin concentrations, leading to increased protein turnover. Overexpression of SLP-2 activates the MEK/ERK signaling pathway, and suppresses the mitochondrial apoptosis pathway, indicating that SLP-2 inhibits apoptosis by activating the MEK/ERK pathway and inhibiting the mitochondrial apoptosis pathway in cervical cancer cells. PMID: 29516570
The expression of STOML2, a gene that plays a key role in mitochondrial function and T-cell activation, is associated with both IL-6 signaling and asthma risk. PMID: 26932604
results reveal an important role of SLP2 membrane scaffolds for the spatial organization of inner membrane proteases regulating mitochondrial dynamics, quality control, and cell survival. PMID: 27737933
Slp2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans PMID: 27986413
The significant association of SLP-2 overexpression with unfavorable clinicopathological characteristics and BRAFV600E mutation indicates that SLP-2 may have a role in aggressiveness of BRAF-mutated papillary thyroid carcinoma. PMID: 26750533
Stomatin-like protein 2 is overexpressed in epithelial ovarian cancer and predicts poor patient survival PMID: 26487491
STOML2 was correlated to progression in cervical cancer, and implicated it as a potential predictive factor for the prognosis of cervical cancer. PMID: 25973071
Our study showed that STOML-2 was negatively regulated by miR1207-5p in esophageal carcinoma PMID: 25695396
SLP-2 was upregulated by TGF-beta1, indicating a possible role of SLP-2 in Papillary thyroid cancer tumorigenesis. PMID: 24190591
STOML2 may have a role in progression of gastric adenocarcinoma PMID: 24258357
Increased levels of SLP-2 correlate with lymph node metastasis in gastric cancer. PMID: 23371255
SLP-2 may play an important role in human GBC tumorigenesis, and SLP-2 might serve as a novel prognostic marker in human GBC. PMID: 23918306
Expression of SLP-2 is associated with invasion of esophageal squamous cell carcinoma. PMID: 23667687
SLP-2 deficiency in T-lymphocytes is associated with abnormal cardiolipin compartmentalization in mitochondrial membranes, defects associated with altered mitochondrial respiration that is increasingly uncoupled from ATP production. PMID: 23028053
[review] Stomatin family member STOML2 is oligomeric; it localizes mostly to membrane domains and has been shown to modulate ion channel activity. PMID: 21501885
SLP-2 facilitates the compartmentalization not only of mitochondrial membranes but also of the plasma membrane into functional microdomains. PMID: 22623988
investigation of biomarkers for early diagnosis of endometriosis: Data suggest that SLP2, tropomyosin 3, and tropomodulin 3 are autoantigens present in blood of women with endometriosis; immunodominant epitopes were identified. PMID: 22158085
SLP-2 and HER2/neu can play a role in lymph node/distant metastases of breast cancers PMID: 22081131
We propose that the function of SLP-2 is to recruit prohibitins to cardiolipin to form cardiolipin-enriched microdomains in which electron transport complexes are optimally assembled. PMID: 21746876
plasma concentrations of stomatin (EPB72)-like 2 in early-stage colorectal cancer patients were elevated as compared with those of healthy individuals PMID: 21209152
The dominant inheritance of hyperphosphorylated paratarg-7 explains cases of familial IgM monoclonal gammopathy of undetermined significance and Waldenstrom macroglobulinemia PMID: 21220746
negatively modulates mitochondrial sodium-calcium exchange PMID: 19944461
SLP-2 overexpression is associated with tumour distant metastasis and poor prognosis in pulmonary squamous cell carcinoma. PMID: 19839737
overexpression of stomatin-like protein 2 is associated with neoplasms PMID: 16533792
SLP-2 is a high abundance protein in several tissues and cells and may play an important biological role; this study uses mass spectrometry to analyze its primary structure PMID: 16671055
SLP-2 was overexpressed in endometrial adenocarcinoma compared with their normal counterparts. PMID: 17342323
High-level SLP-2 expression was associated with decreased overall survival (P = .011) and was more often found in patients with tumors larger than 20 mm, lymph node metastasis, advanced clinical stage, distant metastasis PMID: 17709317
SLP-2 is an important player in T cell activation by ensuring sustained TCR signaling PMID: 18641330
Familial MGUS and multiple myeloma were associated with a dominant inheritance of hyperphosphorylated paratarg-7 PMID: 19767238
Endosymbiotic origin of paraslipin from an alphaprotoebacterial ancestor (SLP-2) PMID: 18267007

Hide All

Subcellular Location

Cell membrane; Peripheral membrane protein. Mitochondrion. Mitochondrion inner membrane; Lipid-anchor. Mitochondrion intermembrane space. Membrane raft. Cytoplasm, cytoskeleton.

Protein Families

Band 7/mec-2 family

Tissue Specificity

Ubiquitously expressed at low levels. Expressed in lymphoid tissues (at protein level).

Database Links

HGNC: 14559

OMIM: 608292

KEGG: hsa:30968

STRING: 9606.ENSP00000348886

UniGene: Hs.3439

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Code	CSB-RA040491A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), A431 whole cell lysate(30µg), A549 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes:SLP 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time：30s IHC image of CSB-RA040491A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA040491A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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STOML2 Recombinant Monoclonal Antibody

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Target Background