Product Details

Uniprot No.

Target Names

Alternative Names

HMG box transcription factor 4 antibody; hTCF 4 antibody; hTCF-4 antibody; T cell factor 4 antibody; T cell specific HMG box antibody; T cell specific transcription factor 4 antibody; T-cell factor 4 antibody; T-cell-specific transcription factor 4 antibody; TCF 4 antibody; TCF-4 antibody; TCF4 antibody; TCF7L2 antibody; TCF7L2 protein antibody; TF7L2_HUMAN antibody; Transcription factor 7 like 2 antibody; Transcription factor 7 like 2 T cell specific HMG box antibody; Transcription factor 7-like 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human TCF7L2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13G5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TCF7L2, also known as TCF-4, serves as a critical transcription factor within the Wnt signaling pathway, where it partners with β-catenin to regulate genes controlling cell proliferation, differentiation, and survival. This pathway's dysregulation has been extensively linked to colorectal cancer progression and type 2 diabetes susceptibility, making TCF7L2 a compelling target for researchers investigating metabolic disease mechanisms and oncogenic signaling networks.

This recombinant monoclonal antibody, clone 13G5, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human TCF7L2 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The result is reliable performance across extended studies where experimental continuity matters.

Validation studies demonstrate robust performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of HeLa cells reveals clear nuclear localization consistent with TCF7L2's role as a transcription factor, with optimal results achieved at dilutions between 1:50 and 1:200. Flow cytometric analysis using A549 cells confirms specific detection with minimal background, showing distinct separation from isotype control when used at 1:100 dilution. Both cell lines were processed using standard fixation with 4% formaldehyde and permeabilization with 0.2% Triton X-100, providing researchers with established protocols for immediate implementation.

This antibody supports investigations into Wnt pathway dynamics, cancer biology, and metabolic disease research where understanding TCF7L2 expression patterns and subcellular localization provides mechanistic insights into disease pathophysiology and potential therapeutic intervention points.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.

Gene References into Functions

Studied association of single nucleotide polymorphisms in Transcription factor 7-like 2 (TCF7L2) gene and genetic predisposition to type 2 diabetes in nonobese patients. PMID: 28263491
The meta-analysis suggested that TCF7L2 polymorphism rs7903146 was significantly associated with type 2 diabetes mellitus in Caucasian, East Asian, South Asian and other ethnicities. [Meta-analysis] PMID: 29514658
The responses of insulin and HOMA-IR to ALE supplementation have shown an interaction with single-nucleotide polymorphism rs7903146 in TCF7L2. PMID: 30177026
The two varients of the gene TCF7L2 are important genetic risk factors for the T2D development in the Han ethnic group in China. PMID: 30266127
In conclusion, homozygous TT allele carriers show altered postprandial triglyceride response, mainly influencing very low density lipoproteins and high density lipoproteins subclasses suggesting a genotype-mediated effect on hepatic lipid regulation. PMID: 28220878
a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID: 29959006
stable knockdown of FOXO3, NCOA3, and TCF7L2 restored growth in low glucose but reduced MEK/MAPK phosphorylation, reduced anchorage-independent growth, and modulated expressions of GLUT1 and Ras pathway related proteins. PMID: 29301589
Study showed the association of TCF7L2 SNPs (rs7903146, rs12255372, and rs10885406) with high total cholesterol/high-density lipoproteins ratio. Findings highlight the influence of TCF7L2 SNPs on altered lipid profile in the Balinese, which may further link to the risk of cardiovascular diseases. PMID: 30027476
TCF7L2 rs7903146 polymorphism may be associated with the susceptibility to diabetic nephropathy in Chinese Han population, but rs290487 is not. The strong linkage disequilibrium existed between the 2 single nucleotide polymorphisms and haplotype T-T (rs7903146-rs290487) increased the susceptibility to diabetic nephropathy. PMID: 30290587
the T risk allele of the rs7903146 in the TCF7L2 gene increases the risk of type 2 diabetes, was determined. PMID: 29631902
Common variation at TCF7L2 influences acute responses to both glipizide and metformin in people without diabetes and highlight altered incretin signaling as a potential mechanism by which TCF7L2 variation increases T2D risk. PMID: 29326107
No correlation between the studied polymorphisms of the TCF7L2 gene and GDM was observed. PMID: 27958632
Type 1 diabetes mellitus patients carriers of the TCF7L2 variant had a milder immunologic and metabolic phenotype at type 1 diabetes diagnosis, which could be partly driven by type 2 diabetes-like pathogenic mechanisms. PMID: 29025879
The SNPs in TCF7L2 and HHEX were genotyped by polymerase chain reaction-restriction fragment length polymorphism. There were no significant differences in the distribution of genotypes and alleles between polycystic ovary syndrome cases and controls PMID: 26563606
rs122555372 was associated with pancreatic reserve in patients with type 2 diabetes and with fasting glucose and beta-cell function in individuals without diabetes PMID: 28101933
TCF7L2 mRNA expression is downregulated in humans with impaired glucose tolerance and adipocyte insulin resistance. PMID: 29317436
PGC-1alpha induction during differentiation is required for both mitochondrial biogenesis and commitment to the hepatocytic lineage, and TCF7L2 repression is sufficient to increase PGC-1alpha expression, mitochondrial biogenesis and OXPHOS activity. PMID: 28795454
role in dermal papilla cell proliferation PMID: 24354472
Silencing the tcf4 gene confers sensitivity to oxaliplatin in colorectal cancer cells. PMID: 24869759
GRbeta bound to the N-terminus domain of TCF-4 its influence on Wnt signaling required both ligand- and DNA-binding domains. This is enough to maintain the TCF/LEF activity at a high level in the absence of beta-catenin stabilization. PMID: 25301232
TRIB2 negatively regulates Wnt activity through a reduction in protein stability of TCF4 and beta-Catenin PMID: 25311538
CtBP physically interacted with TCF-4, and this interaction was significantly inhibited in the presence of MTOB. The above findings point to a novel role of CtBPs in the promotion of CSC growth and self-renewal PMID: 25483087
Our data revealed that low TCF4 protein expression was a useful predictive factor of good tumor response to nCRT and good outcomes in patients with LARC. PMID: 25519018
These results indicate that a dynamic interplay of TCF transcription factors governs MYC gene expression in colorectal cancers. PMID: 25659031
KLF5 facilitates lysophosphatidic acid -induced interaction between beta-catenin and TCF4. PMID: 25683913
The E3 ligase RNF43 inhibits Wnt signaling downstream of mutated beta-catenin by sequestering TCF4 to the nuclear membrane. PMID: 26350900
Results provides evidence that TCF4 and ZEB1 modulate each other's transcriptional activity in the regulation of tumor pro-invasive Wnt target genes. PMID: 26387539
The expression of NLK was negatively correlated with TCF4 expression in lung cancers PMID: 26823848
the beta-catenin/Tcf4 interaction is disrupted by BC-23 PMID: 27014877
Data show that HMG-box transcription factor 1 protein HBP1-mediated elevation of CDK inhibitor p21 through the Mdm2/p53 and TCF4/EZH2 pathways contributes to both cellular senescence and tumor inhibition. PMID: 27129219
These results may provide a linkage between PCa chemoresistance and exosome regulatory networks and thus lead us to propose that AR, PTEN and TCF4 genes may be the important genes which are regulated by exosome miRNAs in chemoresistance cancer cells. PMID: 27278879
G-17 caused the overexpression of beta-catenin and TCF-4. PMID: 27430592
Taken together, these results suggest that this newly identified Rock2-beta-catenin/TCF4-SCARA5 axis will provide novel insight into the understanding of the regulatory mechanisms of proliferation in human RCC. PMID: 27793664
we observed Dickkopf-related protein 3 (DKK3) as a direct target of miR-25 in vitro. Upregulation of DKK3 partially attenuated the oncogenic effect of miR-25 on melanoma cells. Ectopic expression of miR-25 in melanoma cells induced beta-catenin accumulation in nuclear and inhibited TCF4 (T cell factor 4) activity, as well as the expression of c-Myc and Cyclin D1. PMID: 27801786
FOXN3 bind to beta-catenin and inhibited beta-catenin/TCF signaling by blocking the interaction between beta-catenin and TCF4. Loss of FOXN3 in colon cancer activates beta-catenin/TCF signaling and promotes the growth and migration of cancer cells. PMID: 28039460
High TCF4 expression is associated with colorectal cancer. PMID: 28921929
Overexpression of vPK led to reduced mRNA expression of cyclin D1, a well-known transcriptional product of Wnt signaling, suggesting that vPK effectively regulates the host signaling pathway through direct interactions with cellular proteins. PMID: 29432739
TCF7L2 rs290487, rs6585194, and rs7094463 polymorphisms were found to be significantly associated with gestational diabetes mellitus. PMID: 27465520
TCF7L2 rs7903146 polymorphism is associated with ischemic heart disease. PMID: 28299838
The IVS3C>T locus in the TCF7L2 gene is not independently statistically significantly associated with the development of type 2 Diabetes Mellitus in the Kyrgyz population. PMID: 29171469
six of eight SNPs were found to have significant associations between TCF7L2 variants and gestational diabetes mellitus (GDM) risk in the overall population, with the most powerful SNPs being rs7903146, rs12255372 and rs7901695, but the contribution of these SNPs to GDM risk were variable among different racial/ethnic groups - meta-analysis PMID: 28002648
These results suggest rs12573128 is significantly associated with an increased risk of SCZ in the Chinese Han population. PMID: 28404897
The study found that TCF7L2 SNPs (rs1225404 and rs7003146) might be associated with breast cancer risk in Northwest Chinese Han populations. PMID: 27738320
These findings further support the hypothesis that TCF7L2 gene variation contributes to diabetogenesis in a subset of young people with Type 1 diabetes. PMID: 27027642
The rs7903146 variant in the TCF7L2 gene increases the risk of impaired glucose tolerance or type 2 diabetes in obese adolescents by impairing beta-cell function, and hepatic insulin sensitivity predicts the development of impaired glucose tolerance or type 2 diabetes over time. PMID: 28611053
In conclusion, TCF7L2 regulates estradiol- or progesterone-modulated islet and hepatic glucose metabolism. PMID: 27108846
TCF7L2 rs7903146 and 112/112 haplotype of CAPN10 might be associated with gestational diabetes risks.[meta-analysis] PMID: 28277135
Authors found that KIF23 was regulated by TCF-4 at transcriptionally level. Therefore, this evidence indicates KIF23 over-expression is associated with glioma malignancy and conferred a worse survival time in glioma. PMID: 27013586
findings indicate that the association between TCF7L2 SNP rs12255372 and HDL-C may be modified by dietary fat intake in this Asian Indian population PMID: 29182660
Wnt Signaling Pathway Effector TCF7L2 plays a role in Glucose Homeostasis. PMID: 27159876

Hide All

Involvement in disease

Diabetes mellitus, non-insulin-dependent (NIDDM)

Subcellular Location

Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Protein Families

TCF/LEF family

Tissue Specificity

Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived

Database Links

HGNC: 11641

OMIM: 125853

KEGG: hsa:6934

STRING: 9606.ENSP00000444972

UniGene: Hs.593995

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA192658A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of Hela cell with CSB-RA192658A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A549 cells stained with CSB-RA192658A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

TCF7L2 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background