Code | CSB-RA222792A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
The production of the THBD recombinant monoclonal antibody involves a well-structured process. It begins with the isolation of B cells from an immunized animal, where a synthesized peptide derived from human THBD is used as the immunogen. The harvested B cells are then utilized for RNA extraction, followed by reverse transcription to generate cDNA. Through PCR amplification using primers specific to the antibody constant regions, the THBD antibody genes are amplified and inserted into an expression vector. This vector is then transfected into host cells to facilitate antibody expression. The THBD recombinant monoclonal antibodies are collected from the cell culture supernatant and purified using affinity chromatography. To ensure their reliability, the purified THBD recombinant monoclonal antibody undergoes rigorous characterization, including ELISA and IHC analysis, to validate their specificity and binding to human THBD protein.
There are currently no reviews for this product.