Product Details

Uniprot No.

Target Names

Alternative Names

E(Sp1) homolog antibody; Enhancer of split groucho 1 antibody; Enhancer of split groucho like protein 1 antibody; Enhancer of split groucho-like protein 1 antibody; ESG antibody; ESG1 antibody; GRG1 antibody; TLE1 antibody; TLE1_HUMAN antibody; Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody; Transducin like enhancer of split 1 antibody; Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody; Transducin like enhancer protein 1 antibody; Transducin-like enhancer protein 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TLE1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

22D6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TLE1, or Transducin-like enhancer protein 1, functions as a transcriptional corepressor within the Groucho/TLE family, playing essential roles in developmental signaling pathways including Wnt, Notch, and various homeodomain transcription factor networks. This protein has garnered significant attention in diagnostic pathology as a highly sensitive marker for synovial sarcoma, while its broader involvement in cell fate determination and differentiation makes it a valuable target for researchers investigating epigenetic regulation and nuclear signaling mechanisms.

This recombinant monoclonal antibody, clone 22D6, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human TLE1, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies. Affinity chromatography purification delivers a reagent ready for sensitive detection applications.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis detects TLE1 at the expected 83 kDa molecular weight across diverse human cell lines including MCF7, A549, U251, and HEK293, confirming reliable detection in breast cancer, lung adenocarcinoma, glioblastoma, and embryonic kidney models at dilutions ranging from 1:500 to 1:5000. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human stomach tissue and gastric cancer specimens shows clear staining using citrate buffer antigen retrieval at 1:50 to 1:200 dilutions, providing flexibility for optimizing signal intensity in your specific tissue context.

Whether investigating TLE1's role in transcriptional repression, exploring its diagnostic utility in soft tissue tumor classification, or examining its function in developmental pathways, this antibody provides a dependable tool for advancing your epigenetics and nuclear signaling research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Gene References into Functions

The majority of malignant rhabdoid tumors and atypical teratoid/rhabdoid tumor cases showed some TLE1 immunoreactivity (n = 16; 89% for >/=1 + staining); 14 (78%) of total cases showed >/=2 + positivity using any of the 3 scoring systems. Over half (n = 10; 56%) of cases showed >/=2 + staining; 4 (22%) cases showed 3 + strong and diffuse TLE1 staining measured by all scoring systems in agreement. PMID: 29490565
Immunohistochemical analysis for TLE1 can identify basal cell adenomas and basal cell adenocarcinomas by luminal cell staining difference, especially indistinct luminal cell expression for TLE1 in invasive areas of BCAC. PMID: 30053869
TLE1 is a potential immunohistochemical marker for glomus tumors PMID: 28632567
studies indicate Bit1 is an inhibitor of EMT and metastasis in lung cancer and hence can serve as a molecular target in curbing lung cancer aggressiveness PMID: 27655370
Authors present data indicating that TLE1 is rapidly excluded from the nucleus following epidermal growth factor receptor pathway activation, an effect that likely accounts for its inability to mediate effective repression under such conditions. PMID: 28192406
TLE1 is not a stand-alone diagnostic immunohistochemical marker for synovial sarcoma PMID: 27568668
Data suggest TLE1 is highly expressed in macrophages cultured in vitro, in macrophages of atherosclerotic plaques, and in macrophages of visceral adipose tissue from obese women; expression of TLE1 is up-regulated upon macrophage activation. PMID: 26763127
TLE1 has a role in regulating epithelial-to-mesenchymal transition in A549 cells through its repressive effect on E-cadherin PMID: 25446087
There was no difference in TLE-1 staining between different subtypes of synovial sarcoma PMID: 24422953
GrG1 is involved in pancreatic cell differentiation, establishing a monohormonal beta cell identity. Grg1 is the predominant Groucho expressed in human beta-cells but acts functionally similarly to Grg3. PMID: 24487024
TCF/TLE tetramer complex promotes structural transitions of chromatin to mediate repression. PMID: 24596249
highly specific biomarker for synovial sarcoma PMID: 23197007
signaling and increases the expression of recombinant recognition sequence binding protein at the Jkappa site (RBP-J) and transducin-like enhancer of Split (TLE). PMID: 23775085
Although molecular confirmation is the diagnostic gold standard for synovial sarcoma, TLE1, in view of its high sensitivity may be a useful marker within the optimal IHC panel comprising EMA, BCL2, MIC2, CD34 and CK7 PMID: 23287123
TLE1 is selectively upregulated in invasive breast tumors relative to noninvasive ductal carcinoma in situ and normal mammary epithelial tissues. PMID: 22952044
TLE1, is a necessary transcriptional component of the ER complex, where it facilitates ER-chromatin interactions. PMID: 21536917
Epistatic and biological interactions between TLE1 and NOD2 are involved in inflammatory bowel disease pathogenesis. PMID: 21699783
TLE1 is a critical factor for the survival of synovial sarcomas PMID: 21319215
Studies suggest that TLE1 and TLE3 might also play roles independent of HESX1 by interacting with other transcription factors like PROP1. PMID: 20181723
Biologically, loss of TLE-dependent rRNA gene repression coincides with increased global protein synthesis and enhanced cell proliferation PMID: 20160071
Hyperphosphorylation induced by cofactor binding plays a positive role in the regulation of Gro/TLE1 anti-neurogenic activity. PMID: 19956621
The transcription co-repressor TLE1 interacted with the intracellular region of gpl30 through its Q domain PMID: 12030375
1.6 A crystal structure of a C-terminal fragment of human Groucho/TLE1, comprising part of the Ser/Pro-rich region and a seven-bladed beta propeller WD40 repeat domain, implicated in protein-protein interactions PMID: 12057191
Not only PNRC2 but also the corepressor TLE1 functioned as ERRgamma coactivator in a reporter gene analysis. PMID: 14651967
beta-catenin displaces Groucho/TLE from Tcf/Lef by binding to a previously unidentified second, low-affinity binding site on lymphoid enhancer factor 1 PMID: 15768032
ESG1 expression in human cells is studied. PMID: 16166252
TLE is a robust immunohistochemical marker for synovial sarcoma in humans. PMID: 17255769
interaction between Sirt1 and TLE1 is important for mediating repression of NF-kappaB activity PMID: 17680780
Knockdown of TLE1 or TLE4 levels increased the rate of cell division of the AML1-ETO-expressing Kasumi-1 cell line, whereas forced expression of either TLE1 or TLE4 caused apoptosis and cell death. PMID: 18258796
data suggest that TLE1 epigenetic inactivation contributes to the development of hematologic malignancies by disrupting critical differentiation and growth-suppressing pathways PMID: 18519670
Inhibition of cortical neuron differentiation by Groucho/TLE1 requires interaction with WRPW, but not Eh1, repressor peptides PMID: 18611861
Results demonstrate that transcriptional repression by PRH is dependent on TLE availability and suggest that subnuclear localization of TLE plays an important role in transcriptional repression by PRH. PMID: 18713067
TLX1 interacts with TLE1 via an Eh1-like motif. PMID: 19250647
TLE1 expression is by no means specific for synovial sarcoma, being present in a number of tumors, which enter its differential diagnosis. PMID: 19363472
Findings confirm, in a prospective diagnostic context, that TLE1 is more sensitive and specific for synovial sarcoma than any other currently available immunohistochemical stains. PMID: 19809272

Hide All

Subcellular Location

Nucleus. Note=Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.

Protein Families

WD repeat Groucho/TLE family

Tissue Specificity

In all tissues examined, mostly in brain, liver and muscle.

Database Links

HGNC: 11837

OMIM: 600189

KEGG: hsa:7088

STRING: 9606.ENSP00000365682

UniGene: Hs.197320

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Code	CSB-RA279338A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), A549 whole cell lysate(30µg), U251 whole cell lysate(30µg), HEK293 whole cell lysate(30µg) All lanes: TLE1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time：2min IHC image of CSB-RA279338A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA279338A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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