Code | CSB-RA249639A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
CUSABIO meticulously engineered the TMEM192 recombinant monoclonal antibody following a precise protocol. Initially, B cells were extracted from the spleen of an animal immunized with the synthesized peptide derived from human TMEM192. The RNA isolated from these B cells was then reverse-transcribed into cDNA. Using the cDNA as a template, the gene encoding the TMEM192 antibody was amplified with a degenerate primer and inserted into a vector. Through transfection, the recombinant vector was introduced into host cells, enabling efficient expression of the TMEM192 recombinant monoclonal antibodies. These antibodies were subsequently harvested from the cell culture supernatant and underwent purification via affinity chromatography. Rigorous validation, including ELISA, WB, and IHC testings, was conducted to confirm the specific reactivity of the TMEM192 recombinant monoclonal antibody with human TMEM192 protein, ensuring its reliability, precision, and suitability for diverse applications.
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