TMEM192 Recombinant Monoclonal Antibody

Code CSB-RA249639A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
    All lanes: TMEM192 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 31 kDa
    Observed band size: 31 kDa
  • IHC image of CSB-RA249639A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Target Names
TMEM192
Alternative Names
Transmembrane protein 192, TMEM192
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human TMEM192
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
21G8
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO meticulously engineered the TMEM192 recombinant monoclonal antibody following a precise protocol. Initially, B cells were extracted from the spleen of an animal immunized with the synthesized peptide derived from human TMEM192. The RNA isolated from these B cells was then reverse-transcribed into cDNA. Using the cDNA as a template, the gene encoding the TMEM192 antibody was amplified with a degenerate primer and inserted into a vector. Through transfection, the recombinant vector was introduced into host cells, enabling efficient expression of the TMEM192 recombinant monoclonal antibodies. These antibodies were subsequently harvested from the cell culture supernatant and underwent purification via affinity chromatography. Rigorous validation, including ELISA, WB, and IHC testings, was conducted to confirm the specific reactivity of the TMEM192 recombinant monoclonal antibody with human TMEM192 protein, ensuring its reliability, precision, and suitability for diverse applications.

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Target Background

Gene References into Functions
  1. Authors found that transmembrane protein 192 (TMEM192) interacted with TIG1. Authors also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins. PMID: 27989102
  2. TMEM192 deficiency can induce autophagy in tumor cells, and can further activate apoptosis by the mitochondrial pathway through autophagy. PMID: 22736246
  3. TMEM192 was found to be strongly expressed in human kidney, liver, lung and pancreas tissue. The widespread tissue distribution could suggest an important role of TMEM192 for lysosomal function. PMID: 20370317
Subcellular Location
Lysosome membrane; Multi-pass membrane protein. Late endosome.
Protein Families
TMEM192 family
Tissue Specificity
Strongly expressed in kidney, liver, lung and pancreas.
Database Links

HGNC: 26775

KEGG: hsa:201931

STRING: 9606.ENSP00000305069

UniGene: Hs.704511

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