Product Details

Uniprot No.

Target Names

Alternative Names

Tumor necrosis factor alpha-induced protein 3 (TNF alpha-induced protein 3) (EC 2.3.2.-) (EC 3.4.19.12) (OTU domain-containing protein 7C) (Putative DNA-binding protein A20) (Zinc finger protein A20) [Cleaved into: A20p50, A20p37], TNFAIP3, OTUD7C

Species Reactivity

Human

Immunogen

A synthesized peptide from human TNFAIP3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6F11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TNFAIP3, also known as A20, serves as a critical negative regulator of NF-κB signaling and plays an essential role in limiting inflammatory responses. This zinc finger protein possesses dual enzymatic activities—functioning as both a deubiquitinase and an E3 ubiquitin ligase—that enable it to terminate NF-κB activation following TNF-α stimulation. Dysregulation of TNFAIP3 has been implicated in autoimmune disorders, chronic inflammatory conditions, and various malignancies, making it a significant target for researchers investigating immune regulation and inflammatory pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide from human TNFAIP3, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring your results remain comparable across experiments conducted months or years apart.

Validation studies demonstrate reliable performance across multiple applications. Western blot analysis of K562 and Jurkat whole cell lysates reveals a clean band at the predicted 90 kDa molecular weight, confirming specific detection of full-length TNFAIP3 in human lymphoid and myeloid cell lines. Immunofluorescence staining in HeLa cells enables visualization of subcellular localization patterns, while flow cytometry analysis using Raji cells shows clear positive signal separation from isotype controls, supporting intracellular detection studies in B-cell models.

Whether you are investigating TNF-α-induced signaling cascades, exploring ubiquitin-mediated protein regulation, or characterizing TNFAIP3 expression in disease models, this antibody provides the versatility and reliability needed for immunology, oncology, and inflammation research applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.

Gene References into Functions

Analysis of postmortem brain tissue and CSF of multiple sclerosis (MS) patients revealed that expression of A20/TNFAIP3, as well as the expression levels of IL-1beta and NLRP3 were significantly increased in MS plaques. PMID: 29789522
LIFR-AS1 serves as a competitive endogenous RNA for miR-29a to inhibit its expression and up-regulate downstream target TNFAIP3 expression, finally modulating the resistance of colorectal cancer to pohotodynamic therapy. PMID: 29807108
Study demonstrates evidence of the constitutive expression of A20 in macrophages, its role in supporting the differentiation program, and the functional consequences of the zinc finger domains thereof. PMID: 28771803
A20 dimers bind linear ubiquitin to stabilize the Ripoptosome and potentiate its apoptosis-inducing activity. PMID: 30209212
At least in the subcutaneous fat of humans and mice, the levels of PGC-1a decrease during obesity, while its physical association with A20 increases. PMID: 29678181
Results suggest a clinicopathologic implication of A20 in progression of NKTL. PMID: 29380463
Studied effect of temperature on gene expression regulation by NF-kappabeta on TNF alpha induced protein 3 (A20) transcription. PMID: 29760065
TNFAIP3 abnormality was less prevalent among intestinal diffuse large B-cell lymphomas with some discordancy between gene deletion and protein expression. PMID: 29463935
antidepressant treatment exerts anti-inflammatory effects, at least in part through increased expression of the TNFAIP3 gene PMID: 27640899
A20 regulates canonical wnt-signaling through an interaction with RIPK4 PMID: 29718933
these results suggested that in human colorectal cancer cells, A20 may function to inhibit cancer progression via down-regulation of TNFalpha-induced chemokine production by suppression of ERK signaling PMID: 29175508
Low A20 expression is associated with HIV disease. PMID: 29505600
A20 overexpression in vivo significantly reduced renal injury as demonstrated by the improved levels of Scr and BUN and the reduction in histological damage. PMID: 29067462
the risk allele for the TNFAIP3 rs2230926 polymorphism was associated with systemic lupus erythematosus susceptibility. PMID: 29783072
It was concluded that the A20 protein in macrophages modulates lung injury induced by LPS. The overexpression of A20 in macrophages may be involved in modulating macrophage polarization. PMID: 28791391
TNF phase III signalling displays ongoing TNFR1/NF-kappa B activation in monocytic cells. High- and low-sensitive genes are induced including differentially regulated A20. A20 strictly controls this signalling in an IKK- and partially RIP-dependent manner. The A20-mediated control mechanisms are supported by ABIN1 and CYLD. PMID: 28629782
A20 is a negative regulator of inflammation in human myometrium and foetal membranes. PMID: 28911210
The tandem polymorphisms (rs148314165, rs200820567), deletion T followed by a T to A transversion and collectively referred to as TT > A variant of TNFAIP3 may be associated with the susceptibility of chronic hepatitis B virus infection but not the clinical disease. PMID: 28784141
elevated A20 expression is involved in the severity of chronic hepatitis B PMID: 28473659
The results of this study confirmed that the A20 expression is reduced in whole blood of Multiple Sclerosis patients as compared to healthy control. PMID: 28337659
C/EBP beta LAP isoform expression was increased and LIP/TNFAIP3/TNIP1 expression was decreased in systemic lupus erythematosus (SLE) patients. LAP expression was positively correlated with SLE disease activity; TNFAIP3 and TNIP1 expression was negatively correlated with SLE disease activity. PMID: 27659348
A20 may have a role in the progression of chronic HBV infection PMID: 27634895
A20 overexpression inhibits hepatic stellate cell activation, which could be the mechanism for high A20 expression protected livers from fibrosis. PMID: 28251449
The data demonstrate that miR-125b regulates nasopharyngeal carcinoma cell proliferation and apoptosis by targeting A20/NF-kappaB signaling pathway, and miR-125b acts as oncogene, whereas A20 functions as tumor suppressor. PMID: 28569771
we demonstrate that a pathological reduction in TNFAIP3 levels induced NF-kappaB/SMAD7 pathway activation, causing a deficiency in MSCs in ITP patients. The ability of ITP-MSCs to support megakaryocytic differentiation and thrombopoiesis of CD34(+) cells was impaired. PMID: 29327472
miR-125b regulates differentiation and reprogramming of T cell glucose metabolism via targeting A20 in T cell acute lymphoblastic leukemia. PMID: 27637078
Review/Meta-analysis: TNFAIP3 gene rs10499194, rs13207033 polymorphisms decrease the risk of rheumatoid arthritis, especially among Caucasian populations. PMID: 27779104
In vitro deletion of A20 in B cells compromised the expression of IL10. PMID: 27825134
Low A20 expression is associated with hepatocellular carcinoma. PMID: 26909601
The results suggest that TNFAIP3 polymorphisms (rs2230926, rs5029937, rs5029939, and rs3757173) are associated with susceptibility to systemic lupus erythematosus. (Meta-analysis) PMID: 27726311
Hepatitis C virus core protein ligates gC1qR to induce A20 expression in macrophages via P38, JNK and NF-kappaB signaling pathways, which leads to a low-grade chronic inflammation during HCV infection. PMID: 27183919
Results show that TNFAIP3 expression is regulated by miR-19b through targeting its 3'-UTR which contributes to the radioresistance of nasopharyngeal carcinoma and consequently activating the NF-kappaB pathway. PMID: 27919278
demonstrates that TNFAIP3 gene polymorphisms (rs2230926 and rs5029937) are associated with the increased risk of rheumatoid arthritis. [META-ANALYSIS] PMID: 28888761
CHN1 and TNFAIP3 are candidate biomarkers for esophageal squamous cell carcinomas PMID: 27072986
These results identify the ubiquitin-editing enzyme A20 as a novel endogenous mechanism for negative regulation of fibrotic response intensity PMID: 27716397
Our data propose an additional novel mechanism to explain the known NF-kappaB inhibitory effects of A20: by affecting p105 ubiquitination and subsequently its degradation and limited processing. PMID: 28923245
A20 promotes metastasis of aggressive basal-like breast cancers through multi-monoubiquitylation of Snail1. PMID: 28892081
Low TNFAIP3 expression is associated with colitis-associated colorectal cancer. PMID: 27991929
The miR-17-92 is a critical contributor to CML leukemogenesis via targeting A20 and activation of NF-kappaB signaling. PMID: 28461114
IM could upregulate A20 protein to inhibit the activation of NF-kappaB pathway in Jurkat T cells, which was independent of the ABIN1 protein. PMID: 28502291
this meta-analysis confirms that TNFAIP3 gene polymorphisms may play important roles in the pathogenesis of Rheumatoid arthritis PMID: 28199970
Report a significant association between the biased usage of IGHV4-34 (binds to the carbohydrate I/i antigens) and inactivating mutation of TNFAIP3 [encoding a global negative regulator of the canonical nuclear factor-kappaB (NF-kappaB) pathway] in ocular adnexal MALT lymphomas. PMID: 28682481
reduced expression confers susceptibility to psoriasis PMID: 28658319
Haploinsufficiency of TNFAIP3 (A20) by a germline heterozygous mutation leads to the autoimmune lymphoproliferative syndrome (ALPS) phenotype. PMID: 27845235
These results suggested that A20 is involved in regulating intracerebral hemorrhage-induced inflammatory injury in both the central and peripheral system PMID: 27986908
In targeted sequencing, a disruptive mutation of TNFAIP3 was the most common alteration (54%), followed by mutations of TBL1XR1 (18%) and cAMP response element binding proteins (CREBBP) (17%). PMID: 28152507
the DNA fragment containing the associated SNPs interacts through chromatin looping not only with TNFAIP3, but also with IL20RA, located 680 kb upstream. PMID: 27799070
The findings suggest that TNFAIP3 protein may be an independent prognostic marker for poor survival, and a promising target for esophageal squamous cell carcinoma therapy. PMID: 28197630
Study demonstrates that the rs7749323 in the TNFAIP3 gene is strongly associated with the late onset myasthenia gravis(with positive AChR Ab but without thymoma). PMID: 28514294
Influenza a virus NS1 has a role in inducing A20 contributes to viral replication by suppressing interferon-induced antiviral response PMID: 27914808

Hide All

Involvement in disease

Autoinflammatory syndrome, familial, Behcet-like (AISBL)

Subcellular Location

Cytoplasm. Nucleus. Lysosome.; [A20p50]: Cytoplasm.

Protein Families

Peptidase C64 family

Database Links

HGNC: 11896

OMIM: 191163

KEGG: hsa:7128

STRING: 9606.ENSP00000237289

UniGene: Hs.211600

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Code	CSB-RA230008A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: TNFAIP3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time：1min40s Immunofluorescence staining of Hela cell with CSB-RA230008A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Raji cells stained with CSB-RA230008A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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TNFAIP3 Recombinant Monoclonal Antibody

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Target Background