Code | CSB-RA792129A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
FC | 1:20-1:200 |
IP | 1:200-1:1000 |
The production of the recombinant TOP1 antibody depended on Single B Cell technology. There are 3 main steps in the production: 1, Isolation of single B cells. High-throughput methods could be used to obtain the efficient identification and desired specificity of B cells. 11, Single B cell antibody sequencing and cloning. In this step, the antibody gene sequence of TOP1 was obtained and introduced to plasmids, which then would be transferred to mammalian cells for in vitro expression of the TOP1 antibody. 3, Screening of antibodies. The target antibody was obtained in this step. And it has been validated in ELISA, WB, IHC, FC, IP.
TOP1, also known as swivelase or untwisting enzyme, relaxes both positive and negative supercoils by nicking DNA, allowing the broken DNA strand to rotate around the complementary intact strand of the DNA duplex before catalyzing the nick closure. This action is especially crucial during transcription because helical restrictions might obstruct DNA replication and transcription, halting cell growth. TOP1 plays an essential role in normal development in mammals. TOP1 and TOP2A are tumor drivers in a variety of cancers, making them interesting and effective targets for anti-cancer drug development.
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