TOP1, as a topoisomerase, plays a crucial role in DNA replication and transcription. Its unique enzymatic structure allows it to tightly bind to DNA and resolve topological issues arising during these processes by catalyzing DNA unwinding and religation. The primary function of TOP1 is to ensure the smooth progression of DNA double helix during replication and transcription, preventing topological constraints from hindering the process. In terms of signaling pathways, TOP1 interacts and collaborates with other replication and transcription factors in response to the regulatory mechanisms of DNA replication and transcription, jointly maintaining the stability and integrity of the genome. Further exploration of TOP1's structure, function, and signaling pathways holds significant importance for understanding the complex mechanisms of cell biology and genomics, and also provides potential directions for developing new therapeutic strategies and drug targets.
TOP1 Recombinant Monoclonal Antibody
Validated Data
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate
All lanes: TOP1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa
Overlay histogram showing HepG2 cells stained with CSB-RA792129A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating TOP1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA792129A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA792129A0HU(2µg)+ K562 whole cell lysate(500µg)
Lane 3: K562 whole cell lysate (10µg)
IHC image of CSB-RA792129A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA792129A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
The following TOP1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TOP1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA024056GA01HU | TOP1 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA050283 | TOP1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA024056LA01HU | TOP1 Antibody |
Human | ELISA, IF |
CSB-PA024056LB01HU | TOP1 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA024056LC01HU | TOP1 Antibody, FITC conjugated |
Human | |
CSB-PA024056LD01HU | TOP1 Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA792129A0HU | TOP1 Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC, FC, IP |
TOP1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP024056HU CSB-EP024056HU CSB-BP024056HU CSB-MP024056HU CSB-EP024056HU-B |
Recombinant Human DNA topoisomerase 1 (TOP1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-BP024056HU1 | Recombinant Human DNA topoisomerase 1 (TOP1), partial |
Baculovirus |
CSB-BP024056HU1c7 | Recombinant Human DNA topoisomerase 1 (TOP1), partial |
Baculovirus |