TUFM Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA025342MA1HU
Size	US$210
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Image	IHC image of CSB-RA025342MA1HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA025342MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P49411

Target Names

TUFM

Alternative Names

COXPD4 antibody; EF Tu antibody; EF TuMT antibody; EF-Tu antibody; EF-TuMT antibody; EFTu antibody; EFTU_HUMAN antibody; EFTuMT antibody; Elongation factor Tu antibody; Elongation factor Tu mitochondrial antibody; mitochondrial antibody; P43 antibody; Tu translation elongation factor; mitochondrial antibody; Tufm antibody

Species Reactivity

Human

Immunogen

Recombinant Human TUFM protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

15F4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TUFM, or mitochondrial elongation factor Tu, plays an essential role in mitochondrial protein synthesis by delivering aminoacyl-tRNAs to the ribosome during translation. This nuclear-encoded protein is critical for maintaining mitochondrial function, and mutations in TUFM have been linked to combined oxidative phosphorylation deficiency, making it a significant target for researchers investigating mitochondrial biology, metabolic disorders, and cellular energy homeostasis.

This recombinant monoclonal antibody against human TUFM offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence (clone 15F4), this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The human IgG1 isotype format and affinity-chromatography purification ensure high specificity for your target while minimizing background interference.

Validation studies demonstrate robust performance across multiple platforms. In immunohistochemistry, the antibody produces clear staining in paraffin-embedded human stomach tissue when used at dilutions between 1:50 and 1:200 following citrate buffer antigen retrieval. Flow cytometry validation using HeLa cells confirms reliable intracellular detection, with distinct positive signal separation from isotype control when cells are fixed and permeabilized, supporting quantitative analysis of TUFM expression at the single-cell level.

The unconjugated format provides flexibility for researchers to pair this antibody with their preferred detection systems across ELISA, immunohistochemistry, and flow cytometry applications. Whether you are exploring mitochondrial dysfunction in disease models, investigating translational regulation, or characterizing metabolic phenotypes, this TUFM antibody delivers the reproducible performance essential for meaningful research in epigenetics and nuclear signaling pathways.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.

Gene References into Functions

Novel mutation in mitochondrial Elongation Factor EF-Tu associated to dysplastic leukoencephalopathy and defective mitochondrial DNA translation. PMID: 28132884
study revealed a novel role for TUFM as a host restriction factor that exerts an inhibitory effect on avian-signature PB2627E influenza virus propagation in human cells; found that increased TUFM-dependent autophagy correlates with the inhibitory effect on avian-signature influenza virus replication and may serve as a key intrinsic mechanism to restrict avian influenza virus infection in humans PMID: 28611246
High expression of TUFM is associated with colorectal cancer. PMID: 28449687
we identify a novel signaling hub centering on the NLRX1 TUFM protein complex, promoting autophagic flux. Defects in the expression of either NLRX1 or TUFM result in compromised autophagy when treated with EGFR inhibitors.These findings expand our understanding of the components involved in head and neck squamous cell carcinoma autophagy machinery that responds to EGFR inhibitors. PMID: 26876213
TUFM is a novel regulator of epithelial-mesenchymal transition (EMT); there may be a molecular link between mitochondrial dysfunction and EMT induction PMID: 26781467
NLRX1 and TUFM work in concert to reduce cytokine response and augment autophagy. PMID: 23321557
Increased expression of TUFM is a promising new prognostic indicator for colorectal carcinoma. PMID: 22772342
By recruiting Atg5-Atg12 and NLRX1, TUFM serves as a nodal checkpoint of the RIG-I-MAVS axis. It acts similarly to NLRX1 by inhibiting RigI-like-receptor-induced IFN-I but promoting autophagy. PMID: 22749352
Genetic investigation of patients with defective mitochondrial translation led to the discovery of novel mutations in the mitochondrial elongation factor G1 (EFG1) in one affected baby and in the mitochondrial elongation factor Tu (EFTu) in another one PMID: 17160893
Myoblasts isolated from the MELAS patients show A3243G mutation in tRNALeu(UUR) produces a severe respiratory chain deficiency and this phenotype can be partially suppressed by overexpression of EFTu and EFG2. PMID: 18753147
Results suggest that the R336Q mutant mt-EFTu variant fails to bind to aminoacylated mitochondrial tRNAs, thus explaining the observed impairment of mitochondrial translation. PMID: 19524667

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Involvement in disease

Combined oxidative phosphorylation deficiency 4 (COXPD4)

Subcellular Location

Mitochondrion.

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily

Database Links

HGNC: 12420

OMIM: 602389

KEGG: hsa:7284

STRING: 9606.ENSP00000322439

UniGene: Hs.12084

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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