UBA52 Recombinant Monoclonal Antibody

UBA52 encodes a fusion protein that serves as a precursor for two essential cellular components: ubiquitin and the 60S ribosomal protein L40. This unique gene product places UBA52 at the intersection of protein homeostasis and ribosome biogenesis, making it a valuable target for researchers investigating ubiquitin-proteasome pathways, translational machinery, and cellular stress responses.

This recombinant monoclonal antibody, clone 7A2, offers the reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human UBA52, the antibody's sequence-defined nature ensures consistent performance across experiments and eliminates the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms robust detection in human cell lines including HL-60, K562, U-937, Raji, and THP-1, as well as mouse SP2/0 and Raw264.7 cells, with optimal performance at dilutions between 1:500 and 1:2000. The observed band at 10-12 kDa runs slightly below the predicted 15 kDa molecular weight, a difference likely attributable to post-translational processing of the fusion protein or proteolytic cleavage generating the mature ubiquitin and L40 products. Immunohistochemistry validation in human glioma and small intestine tissues demonstrates reliable tissue staining with citrate-based antigen retrieval. Additional confirmation through immunofluorescence in MCF-7 cells and flow cytometry analysis of Jurkat cells provides flexibility for both imaging and quantitative single-cell applications.

Whether you're exploring ubiquitin biology, ribosomal protein function, or cancer-related pathways, this antibody delivers the technical consistency needed for publication-quality results.