UBE2B Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA775975A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:Hela whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), K562 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), U251 whole cell lysate(30µg), A431 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: UBE2B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time：120s IHC image of CSB-RA775975A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA775975A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA775975A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P63146

Target Names

UBE2B

Alternative Names

E2 17kDa antibody; E2 protein antibody; hHR6B antibody; HR6B antibody; RAD6 homolog B antibody; RAD6B antibody; UBC2 antibody; UBE2B antibody; UBE2B_HUMAN antibody; Ubiquitin carrier protein B antibody; Ubiquitin conjugating enzyme E2 B antibody; Ubiquitin conjugating enzyme E2B (RAD6 homolog) antibody; Ubiquitin conjugating enzyme E2B antibody; Ubiquitin protein ligase B antibody; Ubiquitin-conjugating enzyme E2 17 kDa antibody; Ubiquitin-conjugating enzyme E2 B antibody; Ubiquitin-conjugating enzyme E2-17 kDa antibody; Ubiquitin-protein ligase B antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human UBE2B protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14C7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

UBE2B, also known as RAD6B, is a ubiquitin-conjugating enzyme that plays essential roles in DNA damage repair, histone modification, and protein quality control. As a key component of the ubiquitin-proteasome system, UBE2B participates in monoubiquitination of histone H2B, a modification critical for transcriptional regulation and chromatin remodeling. Its involvement in post-replication DNA repair pathways makes it particularly relevant for researchers investigating genome stability, epigenetic mechanisms, and cancer biology.

This recombinant monoclonal antibody, clone 14C7, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human UBE2B, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Affinity purification further enhances performance by reducing background signal.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects a clean band at the predicted 17 kDa molecular weight across diverse human cell lines including HeLa, COLO205, HepG2, K562, MCF7, U251, and A431, as well as mouse NIH/3T3 cells, confirming cross-species reactivity with both human and mouse samples. Immunohistochemistry staining has been validated in paraffin-embedded human spleen tissue and cervical cancer specimens, revealing clear cellular localization patterns. Flow cytometry analysis in HeLa cells shows distinct positive signal separation from isotype controls, supporting intracellular detection applications.

This antibody serves researchers exploring epigenetics and nuclear signaling pathways, offering flexibility across Western blot, immunohistochemistry, flow cytometry, and ELISA platforms within a single validated reagent.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Gene References into Functions

These data deepen insights into the vital role of RAD6/translesion synthesis in platinum drug tolerance and reveal clinical benefits of targeting RAD6 with SMI#9 for managing chemoresistant cancers. PMID: 28490629
These results point to an important role of the affinity between RNF4 and its cognate RAD6B or UBCH5B in governing the multiplicity of substrate ubiquitination. PMID: 27678051
In conclusion, we reveal that miR-455-5p expression is regulated by the TGF-beta-dependent pathway, which subsequently leads to UBE2B down-regulation and contributes to oral cancer tumourigenesis. PMID: 27235675
These findings show the importance of Rad6 in ovarian cancer and emphasize the need for further studies of Rad6 as a potential target for the treatment of ovarian cancer. PMID: 26679603
A novel variant (Chr5.133706925 A > G) residing in the UBE2B gene promoter region confers a high risk for idiopathic azoospermia in a Chinese population. PMID: 26223869
UBE2B mRNA alterations are associated with severe oligozoospermia in infertile men. PMID: 23378580
UBE2B polymorphisms g.-293T>G, g.20016A>G and g.9157A>G are not associated with male infertility, and the GA haplotype is likely a protective factor for male fertility in Northeast Chinese men. PMID: 23079972
beta-catenin and Rad6B interacting regions, were identified. PMID: 22705350
Hydroquinone could upregulate the expression of Rad6B in L-02 hepatic cells. PMID: 19548583
The Rad6-Rad18 enzyme plays an essential role in promoting replication through DNA lesions by translesion synthesis in mammalian cells. PMID: 22547805
Data show that the homodimeric Rad18 RING domain can recruit two Rad6b E2 enzymes, whereas the full-length Rad18 homodimer binds only to a single Rad6b molecule. PMID: 21549715
Ubc2/Rad6 ser(120) regulates ubiquitin-dependent N-end rule targeting by E3{alpha}/Ubr1 PMID: 21041297
Rad6B is a fundamental component of postreplication DNA repair and can be used as an independent marker for determining response to neoadjuvant chemotherapy. PMID: 19466589
MCF10A cells stably transfected with antisense RAD6B display hypersensitivity to damage-inducing drugs. PMID: 14981545
These data reveal a potentially important role for transcriptionally active beta-catenin in the regulation of Rad6B gene expression, and link aberrant beta-catenin signaling with transcriptional deregulation of Rad6B and breast cancer development. PMID: 17050667
Findings reveal a positive regulatory feedback loop between Rad6B and beta-catenin and a novel mechanism of beta-catenin stabilization/activation in breast cancer cells. PMID: 18339854
The SAP domain of RAD18 (residues 248-282) is crucial for binding of RAD18 complexed with RAD6B to DNA substrates. PMID: 18363965
deletions in this suspected promoter region are associated with increased binding affinity for SP1, and might represent one of several factors required for alteration of UBE2B gene expression PMID: 18385908
study suggests that the UBE2B gene is associated with male infertility in Indian men PMID: 18497339

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Subcellular Location

Cell membrane. Nucleus.

Protein Families

Ubiquitin-conjugating enzyme family

Database Links

HGNC: 12473

OMIM: 179095

KEGG: hsa:7320

STRING: 9606.ENSP00000265339

UniGene: Hs.612096

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Ubiquitin mediated proteolysis

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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