CBP Tag Monoclonal Antibody

The CBP tag (Calmodulin Binding Peptide) is a highly efficient affinity and epitope tag derived from skeletal/cardiac muscle myosin light chain kinase 2. Renowned for its strong and specific binding affinity to calmodulin, the CBP tag has become an invaluable tool in advanced protein engineering, particularly for tandem affinity purification (TAP) systems. This dual-tag approach allows researchers to isolate target "bait" proteins along with their native interacting partners under gentle, near-physiological conditions, thereby facilitating the mapping of complex protein networks.

The Mouse Anti-CBP-Tag Monoclonal Antibody (CSB-MA000285, IgG isotype) is meticulously developed to provide high-affinity, monospecific recognition of this calmodulin-binding peptide sequence. Produced via robust hybridoma technology using a recombinant CBP tag protein as the immunogen, this antibody guarantees exceptional specificity, lot-to-lot consistency, and reliable performance even in complex sample matrices.

Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and a high binding affinity. As demonstrated in the Western Blot analysis, the antibody successfully detects the CBP recombinant protein at impressively high working dilutions of 1:5,000 and 1:10,000. The distinct, crisp bands observed on the membrane, with no visible signs of degradation or background interference, underscore the antibody's excellent immunoreactivity and superior signal-to-noise ratio. The consistent detection at both concentrations highlights its remarkable titer, making it an exceptionally cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for proteomic researchers. It serves as a critical reagent for verifying successful CBP fusion protein expression, accurately assessing protein molecular weight, and providing a reliable handle for downstream biochemical assays. With its proven performance, the CBP-Tag Monoclonal Antibody ensures dependable, high-quality results to support cutting-edge research in protein purification, protein-protein interaction studies, and structural biology.