GST Monoclonal Antibody

Datasheet

Code	CSB-MA000031M1m
Size	US$120
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Image	Western Blot Positive WB detected in: 25ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDa Observed band size: 31, 30, 31, 37, 30, 60, 37, 61 KDa Exposure time：5min Western Blot Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26, 102.9, 75.6 KDa Observed band size: 26, 103, 76 KDa Exposure time：1min Western Blot Positive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng All lanes: GST antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa Exposure time：5min Western Blot Positive WB detected in: 25ng recombinant protein with GST tag from E.coli GST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa Exposure time：5min Immunofluorescence staining of 293F transfected cells with CSB-MA000031M1m at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunoprecipitating GST in transfected-293F whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000031M1m in 293F cell lysate Transfected with GST Lane 2: CSB-MA000031M1m (5µg) + Transfected-293F whole cell lysate (500µg) Lane 3: Transfected-293F whole cell lysate (10µg) For western blotting, the blot was detected with CSB-MA000031M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 Overlay Peak curve showing 293F cells transfected with GST stained with CSB-MA000031M1m (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Product Details

Target Names

GST

Alternative Names

Glutathione S-Transferase, gst, GST tag

Raised in

Mouse

Species Reactivity

N/A

Immunogen

Recombinant GST Protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG2b

Clone No.

3D3E4

Purification Method

>95%, Protein A purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:10000-1:640000
IF	1:100-1:300
FC	1:100-1:300
IP	1ul-5ul

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The glutathione S-transferase (GST) tag is a widely adopted fusion tag that streamlines recombinant protein expression, affinity purification, and target detection in molecular and cellular biology research. CSB-MA000031M1m is a mouse monoclonal IgG2b tag antibody raised against recombinant GST protein, purified via Protein A affinity chromatography with purity above 95% to ensure consistent lot-to-lot performance and minimal non-specific background interference. As a high-specificity tag antibody with excellent batch stability, it delivers reliable and reproducible results across multiple experimental platforms. Western blot validation confirms specific recognition of various GST-fused recombinant proteins spanning a broad molecular weight range, producing sharp, clean bands with high sensitivity down to 1.5625 ng and retaining distinct signals even at an ultra-high dilution of 1:640000. It generates bright specific intracellular fluorescence in GST-transfected 293F cells for immunofluorescence, efficiently enriches target GST-tagged protein in immunoprecipitation, and clearly differentiates positive cell populations from isotype controls in flow cytometry. Validated for ELISA, WB, IF, IP and FC, this GST monoclonal antibody offers flexible working dilution ranges to support diverse workflows for GST-fused recombinant protein characterization, purification and subcellular localization studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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