Code | CSB-MA000031M1m |
Size | US$120 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:10000-1:640000 |
IF | 1:100-1:300 |
FC | 1:100-1:300 |
IP | 1ul-5ul |
The glutathione S transferase (GST) monoclonal antibody is secreted from the hybridoma formed by the fusion of mouse myeloma cells and splenocytes from mice immunized with the recombinant GST protein. It is purified from mouse ascites through protein A, and its purity reaches over 95%. This unconjugated GST monoclonal antibody is matched with the mouse IgG2b isotype. It is recommended for use in several scientific applications, including ELISA, WB, IF, FC, and IP applications, and can detect the GST tag from all species.
GST is a 26 kDa protein that naturally occurs in eukaryotic cells. GSTs can catalyze the binding of exogenous substances to glutathione (GSH), thereby detoxifying many environmental toxins including chemotherapy drugs, residual drugs, herbicides and carcinogens. Many researchers integrate GST DNA sequences into expression vectors to produce fusion proteins with GST tags. GST can quickly fold and be stable and has good solubility, so it can promote the expression and dissolution of the fusion protein. In addition, the binding between GST and its substrate GSH can also be used for protein purification and detection. At present, GST-tagged proteins are used in recombinant protein expression, affinity purification, antigen immunity, vaccine research, and pull-down, affinity chromatography and ELISA in a variety of research technology experiments.
There are currently no reviews for this product.