His-Tag Monoclonal Antibody

The His-tag is a cornerstone affinity label in modern protein engineering and structural biology. Its principal strength lies in its remarkable aptitude for facilitating efficient metal-chelate affinity purification, primarily via immobilized nickel-nitrilotriacetic acid (Ni-NTA) chromatography.

The Mouse Anti-His-Tag Monoclonal Antibody (CSB-MA000178, IgG subclass) offers exquisite specificity and robust binding for the detection of recombinant constructs. Generated through rigorous hybridoma technology utilizing a synthetic peptide immunogen, this reagent undergoes stringent affinity-chromatography purification. This sophisticated process ensures the elimination of non-specific immunoglobulins, thereby delivering unparalleled lot-to-lot consistency and minimal background interference in complex sample matrices.

Validated for Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB), this antibody exhibits extraordinary sensitivity. As vividly demonstrated in the accompanying validation data, the antibody achieves unambiguous detection of the His-tagged recombinant protein even at exceptionally high working dilutions of 1:100,000 (Lane 1) and 1:200,000 (Lane 2). The presence of intense, well-defined bands at these high dilutions, conspicuously devoid of smearing or non-specific cross-reactivity, attests to the antibody's superior immunoreactivity and impressive signal-to-noise characteristics. Such high titers render it a remarkably economical yet powerful tool for high-throughput screening and routine diagnostic applications.

This monoclonal antibody serves as an indispensable reagent for verifying recombinant protein expression, assessing molecular weight, and monitoring the efficiency of affinity purification protocols. Whether employed for basic research in gene function elucidation, the development of biotherapeutics, or structural proteomics, the His-Tag Monoclonal Antibody guarantees dependable, high-fidelity results to propel scientific discovery.