MBP Monoclonal Antibody

Code CSB-MA000061M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
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  • MBP-tagged fusion protein(20ng/ml) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000061M0m at dilution of
    Lane 1:1000 Lane 4:8000 Lane 7:64000
    Lane 2:2000 Lane 5:16000 Lane 8:128000
    Lane 3:4000 Lane 6:32000 Lane 9:256000
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size:50kd
    Observed band size:50kd

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen MBP-Tag fusion protein
Raised in mouse
Tested Applications ELISA,WB;Recommended dilution:WB:1:500-1:5000
Relevance Maltose-Binding Protein (MBP) is a native E. coli protein and is part of a complex responsible for the uptake and efficient catabolism of maltodextrins. MBP is composed of 370 amino acid residues. As a fusion tag, MBP is useful for recombinant protein purification by affinity chromatography. It is also used to increase the expression level and solubility of the tagged protein, and for promoting proper folding of the attached protein. MBP increases solubility and the tag is thus particularly useful for recombinant proteins that may otherwise accumulate in an insoluble forms as inclusion bodies. Affinity purification takes place under physiological conditions and mild elution is performed using maltose. The mild elution conditions preserve the activity of the MBP-tagged protein.
Form liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%,Protein G purified
Isotype IgG2a
Clonality monoclonal
Alias Maltose Binding Protein, MBP, MBP Tag
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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