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| Code | CSB-RA846828A0ENV |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:5000 |
The production of a recombinant monoclonal antibody against malE commenced with the immunization of a rabbit using a synthesized peptide derived from Escherichia coli malE. B cells were then isolated from the immunized rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, serving as a template for extending malE antibody genes using degenerate primers. These engineered malE antibody genes were incorporated into a plasmid vector and introduced into host cells for expression. The malE recombinant monoclonal antibody was subsequently isolated from the cell culture supernatant through affinity chromatography and subjected to ELISA and FC applications, displaying specific reactivity with Escherichia coli and human malE protein.
malE, also known as maltose-binding protein (MBP), is a protein found in bacteria, particularly in the Escherichia coli bacterium. Its main role is to function as a periplasmic protein that binds to and transports maltose and related sugar molecules into the bacterial cell.
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KEGG: ecj:JW3994
STRING: 316385.ECDH10B_4223
