Recombinant Arabidopsis thaliana GDP-Man:Man (3)GlcNAc (2)-PP-Dol alpha-1,2-mannosyltransferase (ALG11)

Code CSB-CF894498DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
ALG11; LEW3; At2g40190; T07M07.7; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Alpha-1,2-mannosyltransferase ALG11; Asparagine-linked glycosylation protein 11; Protein LEAF WILTING 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-463
Target Protein Sequence
MAIYFILYTLLTIIFAVSLSLFLSVINARKSRKRAVGFFHPYTNDGGGGERVLWCAVKAI QEENPDLDCVIFTGDHDSSSDSLARRAVDRFGVHLQSPPKVIHLNKRKWIEESTYPHFTM IGQSLGSVYLAWEALRMFTPLYFLDTSGYAFTYPLARIFGCKVVCYTHYPTISLDMISRV RQRNSMYNNDASIAKSNWLSTCKLVYYRAFSWMYGMVGSCTHLAMVNSSWTKSHIEVLWR IPERITRVYPPCDTSGLQAFPLERSSDPPKIISVAQFRPEKAHMLQLEAFSLALEKLDAD VPRPKLQFVGSCRNNSDEERLQKLKDRAVELKVDGDVQFYKNAMYRELVELLGNAVAGLH GMIDEHFGISVVEYMAAGAIPIAHNSAGPKMDIVLEEDGQKTGFLAETVEEYAEAILEIV KMNETERLKMAESARKRAARFSEQRFCEDFKTAIRPIFTGPLK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.
Gene References into Functions
  1. Positional cloning determined that lrg1 affects an alpha-1,2-mannosyltransferase gene, LEW3, involved in protein N-glycosylation. The lrg1 mutation causes a synonymous substitution that alters the correct splicing of the fourth intron in LEW3, causing a mix of wild-type and truncated protein. LRG1 RNA missplicing in roots and short root phenotypes in lrg1 are light-intensity dependent. PMID: 29106622
  2. The lew3 mutation reduces protein glycosylation, impairs cellulose synthesis, and results in xylem collapse. lew3 mutants are more sensitive to salt stress and transpire less water than the wild-type. PMID: 19732381
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Glycosyltransferase 4 family
Tissue Specificity
Expressed in shoots, leaves, sepals, filaments, siliques, stems, roots and guard cells.
Database Links

KEGG: ath:AT2G40190

STRING: 3702.AT2G40190.1

UniGene: At.21597

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