Recombinant Human Protein transport protein Sec61 subunit beta (SEC61B)

Code CSB-CF020958HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
SEC61B
Uniprot No.
Alternative Names
SEC61B; Protein transport protein Sec61 subunit beta
Species
Homo sapiens (Human)
Expression Region
2-96
Target Protein Sequence
PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Component of a ribosome-associated ER translocon complex involved in multi-pass membrane protein transport into the ER membrane and biogenesis. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER. Required for PKD1/Polycystin-1 biogenesis.
Gene References into Functions
  1. used whole exome sequencing in a discovery cohort of 102 unrelated patients who were excluded for mutations in the 2 most common polycystic liver disease genes, PRKCSH and SEC63, to identify heterozygous loss-of-function mutations in 3 additional genes, ALG8, GANAB, and SEC61B. Similarly to PRKCSH and SEC63, these genes encode proteins that are integral to the protein biogenesis pathway in the endoplasmic reticulum. PMID: 28375157
  2. Sec61beta overexpression increased tight junction modulation rates, in conjunction with enhanced delivery of claudin-4 from and to plasma membranes. PMID: 24882410
  3. Sec61beta-KD cells also exhibited altered ATP7A cellular distribution. PMID: 22710939
  4. SEC61beta and its autoantibody as biomarkers for colorectal cancer PMID: 21255561
  5. Sec61beta function provides an alternative pathway for nuclear transport that can be utilized by membrane-embedded proteins such as full-length EGFR. PMID: 20937808
  6. This indicates that EGF receptors are trafficked from the endoplasmic reticulum to the nucleus by a novel pathway that involves the Sec61 translocon. PMID: 17215517

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein.
Protein Families
SEC61-beta family
Database Links

HGNC: 16993

OMIM: 609214

KEGG: hsa:10952

STRING: 9606.ENSP00000223641

UniGene: Hs.191887

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