DGKD Antibody

Code CSB-PA002129
Size US$100
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  • Western Blot analysis of HuvEc cells using DGK-δ Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
DGKD
Alternative Names
DGKD antibody; KIAA0145Diacylglycerol kinase delta antibody; DAG kinase delta antibody; EC 2.7.1.107 antibody; 130 kDa diacylglycerol kinase antibody; Diglyceride kinase delta antibody; DGK-delta antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Synthesized peptide derived from the N-terminal region of Human DGK-δ.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development. May also regulate clathrin-dependent endocytosis.
Gene References into Functions
  1. purification and characterization of DGKepsilon's enzymatic and conformational properties PMID: 28199087
  2. Diacylglycerol signaling limits Akt activation through diacylglycerol kinase delta and PHLPP2. PMID: 23184957
  3. This study demonistrated that the lack of association of DGKD SNPs with PD in the Han Chinese population. PMID: 22406787
  4. Zinc site mutations impair DGKdelta localization to cytoplasmic puncta and enhance localization the plasma membrane. PMID: 20857926
  5. Data indicate a novel mechanism where diacylglycerol kinase delta and protein kinase Calpha modulate the levels of ubiquitinated epidermal growth factor receptors through Akt and ubiquitin-specific protease 8. PMID: 20064931
  6. Alternative splicing of the gene generates two isoforms differing in their expression patterns and in regulatory functions PMID: 12200442
  7. Phosphorylation is involved in the control of subcellular localization of DGKdelta1. PMID: 15228384
  8. Disruption of diacylglycerol kinase delta (DGKD) is associated with seizures in humans and mice. PMID: 17357084
  9. Study identified reduced diacylglycerol kinase delta (DGKdelta) expression and DGK activity in skeletal muscle from type 2 diabetic patients. PMID: 18267070
  10. Polymerization of DGK delta regulates the activity of the enzyme by sequestering DGK delta in an inactive cellular location. PMID: 18334213

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Subcellular Location
Membrane, clathrin-coated pit. Cytoplasm.; [Isoform 1]: Cell membrane; Peripheral membrane protein. Cytoplasm.
Protein Families
Eukaryotic diacylglycerol kinase family
Tissue Specificity
[Isoform 2]: Widely expressed.; [Isoform 1]: Only detected in ovary, and to a lesser extent in spleen.
Database Links

HGNC: 2851

OMIM: 601826

KEGG: hsa:8527

STRING: 9606.ENSP00000264057

UniGene: Hs.471675

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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