Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
The ED50 as determined by its ability to bind Human Galectin-3 in functional ELISA is less than 20 ng/ml.
Alternative Names
LAMP1; Lysosome-associated membrane glycoprotein 1; LAMP-1; Lysosome-associated membrane protein 1; CD107 antigen-like family member A; CD antigen CD107a
Species
Homo sapiens (Human)
Expression Region
29-382aa
Complete Sequence
AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM
Tag Info
C-terminal hFc-tagged
Buffer
Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This recombinant human LAMP1 protein is produced in mammalian cells and contains an amino acid sequence corresponding to the region 29-382aa of the human LAMP1 protein. The product has a C-terminal hFc-tag and is supplied as a lyophilized powder, which can be reconstituted in sterile water or buffer before use. The purity of the protein is greater than 95%, as determined by SDS-PAGE, and the endotoxin level is less than 1.0 EU/µg, as measured by the LAL method. The ED50, as determined by its ability to bind human Galectin-3 in a functional ELISA, is less than 20 ng/ml, making it suitable for various research applications in the field of cancer.
Lysosomal-associated membrane protein 1 (LAMP1) is a highly glycosylated type I transmembrane protein, predominantly located in the lysosomal membrane, and has been implicated in various cellular processes such as lysosome biogenesis, autophagy, and membrane trafficking. Due to its high expression levels in certain cancer cells and its role in cancer progression, LAMP1 is considered an important molecule for cancer research.