Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

The ED50 as determined by its ability to bind Human Galectin-3 in functional ELISA is less than 20 ng/ml.

Target Names

LAMP1

Uniprot No.

P11279

Research Area

Cancer

Alternative Names

LAMP1; Lysosome-associated membrane glycoprotein 1; LAMP-1; Lysosome-associated membrane protein 1; CD107 antigen-like family member A; CD antigen CD107a

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

29-382aa

Complete Sequence

AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM

Mol. Weight

65.5 kDa

Protein Length

Partial

Tag Info

C-terminal hFc-tagged

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant human LAMP1 protein is produced in mammalian cells and contains an amino acid sequence corresponding to the region 29-382aa of the human LAMP1 protein. The product has a C-terminal hFc-tag and is supplied as a lyophilized powder, which can be reconstituted in sterile water or buffer before use. The purity of the protein is greater than 95%, as determined by SDS-PAGE, and the endotoxin level is less than 1.0 EU/µg, as measured by the LAL method. The ED₅₀, as determined by its ability to bind human Galectin-3 in a functional ELISA, is less than 20 ng/ml, making it suitable for various research applications in the field of cancer.

Lysosomal-associated membrane protein 1 (LAMP1) is a highly glycosylated type I transmembrane protein, predominantly located in the lysosomal membrane, and has been implicated in various cellular processes such as lysosome biogenesis, autophagy, and membrane trafficking. Due to its high expression levels in certain cancer cells and its role in cancer progression, LAMP1 is considered an important molecule for cancer research.

Target Background

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, autophagy, and cholesterol homeostasis. Plays also an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.; (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes.; (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Gene References into Functions

LIN28A inhibits lysosomeassociated membrane glycoprotein 1 protein expression in embryonic stem and bladder cancer cells. PMID: 29749495
A novel data on LAMP-1 overexpression in high grade glioma are presented suggesting involvement of this gene and protein in cell adhesion and tumor progression. PMID: 29920782
LAMP1 is involved in the TNM stages and histological differentiation of the esophageal squamous cell carcinoma PMID: 28687162
LAMP1 has a role in fratricide amongst T cell receptor transgenic CD8+ T cells directed against tumor-associated antigens PMID: 27447745
Impaired autophagic pathways were further shown by analyzing late autophagic vesicles; immunostaining with lysosome-associated membrane protein 1 (LAMP-1) antibody revealed enlarged and annular LAMP-1-positive organelles in AMD retinal pigment epithelium (RPE)as opposed to smaller discrete puncta observed in normal RPE PMID: 28055007
The authors show here that human LAMP1 and LAMP2 bind cholesterol in a manner that buries the cholesterol 3beta-hydroxyl group; they also bind tightly to NPC1 and NPC2 proteins that export cholesterol from lysosomes. PMID: 27664420
downregulation of FUT1, which leads to the perinuclear localization of LAMP-1 and 2, is correlated with increased rate of autophagic flux by decreasing mTOR signaling and increasing autolysosome formation. PMID: 27560716
the assembly modes of LAMP-1 and LAMP-2 are different, which may underlie their distinct functions PMID: 27663661
Overexpression of LAMP1 was observed in prostate cancer (PCa) and castration-resistant prostate cancer clinical specimens. Moreover, downstream pathways were identified using si-LAMP1-transfected cells. The discovery of tumor-suppressive miR320a-mediated pathways may provide important insights into the potential mechanisms of PCa metastasis. PMID: 27212625
The data implied that high LAMP1 expression is associated with unfavourable prognosis in laryngeal squamous cell carcinoma patients, and LAMP1 may be identified as a novel prognostic biomarker PMID: 27788920
The data support a viral entry mechanism dependent on binding to the lysosome-resident receptor LAMP1 and further dissociation of the membrane-distal GP1 subunits. PMID: 26849049
A unique triad of histidines of Lassa Virus GP1 forms a binding site for host LAMP1. PMID: 25972533
The LAMP-1 had preferential localization in the high density secondary lysosomes where endogenous human LAMP-1 was enriched. In contrast, a major portion of I382L was located in a low density fraction. PMID: 24695761
Downregulation of miR-184 was consistent with significantly lower levels of LAMP-1 PMID: 25251993
Synergistic defects of different molecules in the cytotoxic pathway lead to clinical familial hemophagocytic lymphohistiocytosis. PMID: 24916509
Caveolin-1 associated adenovirus entry into human corneal cells. PMID: 24147000
study has shown that Lassa virus entry requires a pH-regulated engagement of alpha-DG and LAMP1 both of which need to be glycosylated PMID: 24970085
in TB pleurisy regulatory T lymphocytes effectively inhibit CD107a expression PMID: 24134738
LAMP-1 and LAMP-2 could be used as additional markers with which to assess enzyme replacement therapy effectiveness in Fabry disease. PMID: 24334114
LAMP-1/GFAP showed pronounced co-expression and LAMP-1/CD133 was co-expressed as well in astrocytomas suggesting that tumor cells including the proposed tumor stem cells contain lysosomes. PMID: 23826410
CD107a/LAMP-1 has a role in the protection of NK cells from degranulation-associated suicide PMID: 23847195
Data indicate that monoclonal antibodies specific to CD107a (LAMP-1) or CD107b (LAMP-2) enhanced LPS-induced IL-8 secretion of THP-1 cells. PMID: 23603048
LAMP1 is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity. PMID: 23632890
Simultaneous expression of LAMP1 and EGFR correlates with the ovarian neoplasm grading. PMID: 23172893
LAMP proteins retain TAPL on the limiting membrane of endosomes and prevent its sorting to intraluminal vesicles. PMID: 22641697
Data indicate that lysosomally targeted cameleon Ca2+ probe LAMP1-YCaM accurately and sensitively responds to both global and lysosome-specific Ca2+ signals. PMID: 23098255
Report expression of NKG2D and CD107 in CD8(+) effector memory lymphocytes in Churg-Strauss syndrome. PMID: 22640649
nucleolin and LAMP-1 have a role in promoting infection of human monocytes by Francisella tularensis PMID: 21152024
Human full-length amelogenin increases the proliferation of mesenchymal stem cells by interaction with LAMP1 through the MAPK-ERK signaling pathway. PMID: 20967466
Immunohistochemistry studies showed EGBs to exhibit pronounced reactivity to antibodies against lysosome-associated membrane proteins (LAMP)-1 and LAMP-2, and the lysosomal enzyme cathepsin D. PMID: 20926008
review of structure, function, cell and tissue distribution, intracellular trafficking PMID: 12144129
This study identifies two cDNA clones that code for heart extracellular matrix protein, hLAMP-1. Immunohistochemical studies indicate that the myocardial layer of the heart produces hLAMP-1. PMID: 15052658
Using human leukemia and lymphoma cell lines, we observed a close correlation between CD107a surface expression and target cell lysis, indicating that NK cell cytotoxicity can be assessed by this method. PMID: 15744340
Data identify CD13, CD107a, and CD164 as novel basophil-activation antigens. PMID: 15916720
Novel evidence for differential expression of HBGA and LAMPs in proliferative and involutive phases of immunohistochemistry is presented. PMID: 16570122
expression in keratinocytes cultured at different cell densities in order to induce differentiation PMID: 16710742
Enhanced expression of lysosome-associated membrane protein-1 in melanoma may promote invasion by influencing both adhesion to extracellular matrix and perhaps also binding to endothelial cells. PMID: 16718270
CD107a surface expression has a role in Munc13-4 defect in familial hemophagocytic lymphohistiocytosis PMID: 16778144
LAMP-1 and DC-LAMP antigen chimeras follow different trafficking pathways, induce distinct modulatory immune responses, and are able to present cryptic epitopes. PMID: 16887987
LAMP-1 and LAMP-2 may have roles in accidental involution of the thymic gland PMID: 17048695
Data show that cells lacking either LAMP-1 or LAMP-2 alone formed phagosomes that gradually acquired microbicidal activity and curtailed bacterial growth, but LAMP-1 and LAMP-2 double-deficient cells failed to kill engulfed Neisseria gonorrhoeae. PMID: 17506821
CD107a expression may be a sensitive marker for the cytotoxic activity determination PMID: 18835598
Despite its abundance, LAMP-1 is not essential, but LAMP-2 may be partially important for the Salmonella-containing vacuolar membrane. PMID: 18958159
the activation-induced degranulation of Fas ligand has distinct requirements and involves different mechanisms than those of the granule markers CD63 and CD107a/Lamp-1 PMID: 19079288
Most Hassal's corpuscules in thymoma were negative for LAMPs, but positive in normal thymus. Both lymphocytes and epithelial cells in pathological thymus showed higher intensity for LAMP-2 compared with LAMP-1. PMID: 19343823
Data show that The cell surface expression levels of (ICAM)-2 and -3 on the apoptotic cells were markedly lower, while those of calnexin, calreticulin, and (LAMP)-1 and -2 were significantly higher compared to non-apoptotic cells. PMID: 19524015

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Cytolytic granule membrane; Single-pass type I membrane protein.

Protein Families

LAMP family

Database Links

HGNC: 6499

OMIM: 153330

KEGG: hsa:3916

STRING: 9606.ENSP00000333298

UniGene: Hs.494419

Code	CSB-AP005091HU
MSDS	MSDS Datasheet
Size	$184
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Lysosome-associated membrane glycoprotein 1 (LAMP1), partial (Active)

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