Recombinant Human C-C motif chemokine 5 protein(CCL5) (Active)

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Code CSB-AP000871HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.
Target Names CCL5
Uniprot No. P13501
Research Area Immunology
Alternative Names Beta chemokine RANTES; Beta chemokine RANTES precursor; C C motif chemokine 5; CCL 5; CCL5; CCL5_HUMAN; Chemokine (C C motif) ligand 5; Chemokine CC Motif Ligand 5; D17S136E; EoCP; Eosinophil chemotactic cytokine; MGC17164; RANTES(4-68); Regulated upon activation normally T expressed and presumably secreted; SCYA 5; SCYA5; SIS delta; SIS-delta; SISd; Small inducible cytokine A5 (RANTES); Small inducible cytokine A5; Small inducible cytokine subfamily A (Cys Cys) member 5; Small-inducible cytokine A5; T cell specific protein p288; T cell specific protein RANTES; T cell specific RANTES protein; T cell-specific protein P228; T-cell-specific protein RANTES; TCP 228; TCP228
Species Homo sapiens (Human)
Source E.coli
Expression Region 24-91aa
Complete Sequence SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
Mol. Weight 7.8 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils
Gene References into Functions
  1. These CCL5 derivatives may now be tested against several inflammation-related pathologies where the CCL5:CCR5 axis plays a relevant role. PMID: 29382912
  2. The CCL5 In1.1T/C polymorphism may modulate pulmonary early-onset tuberculosis risk. PMID: 29608337
  3. We use CPRC prostate cancer model and demonstrate that endothelial cells secrete large amount of CCL5 and induces autophagy by suppressing AR expression in prostate cancer cell lines. Consequently, elevated autophagy accelerates focal adhesions proteins disassembly and promoted prostate cancer invasion. Inhibition of both CCL5/CCR5 signaling and autophagy significantly reduces metastasis in vivo PMID: 30200999
  4. CCL5, from endothelial cells, acts in a paracrine fashion on triple-negative breast cancer (TNBC) cells to enhance their migration, invasion, and metastasis. CCL5, in turn, accelerates TNBC cell secretion of PAI-1 and promotes TNBC cell metastasis, thus forming a positive feedback loop. Moreover, this enhanced metastatic ability is reversible and dependent on CCL5 signaling via the chemokine receptor, CCR5. PMID: 28899878
  5. high concentration of plasma CCL5 may promote EMT of breast cancer cells. Plasma CCL5 could be a promised candidate to predict chemotherapy response in NCT of LABC. PMID: 29758926
  6. The polymorphism of CCR1 rs3733096 and CCL5 rs3817656 are associated with spontaneous clearance of hepatitis C virus in Chinese Han population. PMID: 29703961
  7. Our results suggested that the CCL5 level was influenced collectively not only by the genotypes of -403G>A SNP and bacillary load but also by the treatment. Thus, CCL5 may be considered for the development of a diagnostic marker and also as an indicator of recovery. PMID: 29664036
  8. serum levels in active vitiligo significantly elevated compared to those in stable vitiligo patients PMID: 29115683
  9. these findings collectively indicate that TGF-beta regulates CCL5 expression in a stage-dependent manner during breast cancer progression PMID: 29107385
  10. KLF5-regulating cancer-associated fibroblasts affect gastric cancer cells progression by CCL5 secretion and activation of CCR5. PMID: 28934010
  11. Data show that plasminogen activator inhibitor-1 (PAI-1) and chemokine CCL5 (CCL5) overexpression promoted cell proliferation and migration in breast cancer cells. PMID: 29601121
  12. Among infants with lower CCL5 levels, the Haemophilus-dominant microbiota profile was associated with a higher risk of intensive care use and hospital length-of-stay >/=3 days compared to the Moraxella-dominant profile. Conversely, among those with higher CCL5 levels, there were no significant associations between the microbiota profiles and these severity outcomes PMID: 28306146
  13. The current study suggests that TLR3 signaling induces CCL5 expression via NF-kappaB and IRF3 in bile duct cells, and this pathway may be involved in the pathogenesis of BA. PMID: 29070776
  14. Study demonstrates that increased CCL5 expression was restricted to human mesenchymal glioblastoma (GBM) and suggests that CCL5 functions in an autocrine growth-promoting circuit, and establish a new receptor responsible for CCL5 function in mesenchymal glioblastoma cells. PMID: 28380429
  15. Study showed that bone stromal cells promoted prostate cancer progression through the secretion of CCL5. In vitro co-culture of bone stromal cells with prostate cancer cells induced the expression of CCL5, which promoted prostate cancer cell migration. CCL5 was found to have a key role in the progression of prostate cancer in the bone metastasis microenvironment. PMID: 29288523
  16. identifies the essential role of the chemoattractive cytokine CCL5 for liver disease progression and especially hepatocellular carcinoma development PMID: 28011329
  17. Breast cancer cell CCL5 mediates bone marrow independent angiogenesis via paracrine signaling. PMID: 27863423
  18. the present study has demonstrated a novel pathway involving CCl5/CCR1/beta-catenin/Slug, via which human Mesenchymal stem cells promotes colorectal cancer development. PMID: 28542126
  19. The present study suggest that TT genotype of CCL5 In1.1T/C (rs2280789) polymorphism play an important role to increased CCL5 expression in T cell which may enhanced Th1 immunity and help in protection against tuberculosis PMID: 28336310
  20. CSF levels of RANTES were remarkably high only in active multiple sclerosis patients. RANTES levels were associated with transcranial magnetic stimulation measures of cortical synaptic excitability, but not with long-term potentiation (LTP)-like plasticity. PMID: 26733422
  21. we document for the first time that CCL5 induces tumor lymphangiogenesis by the induction of VEGF-C in human cancer cells PMID: 27166194
  22. Our findings indicate that the -403 G/A RANTES (CCL5) promoter gene polymorphism is connected with psoriasis vulgaris disease severity. PMID: 27859608
  23. Baseline serum CCL5 levels and decrease of the serum VEGF-A levels may serve as potential predictive markers for survival or treatment-specific toxicities in metastatic colorectal cancer patients receiving regorafenib. PMID: 27166185
  24. This meta-analysis suggests that RANTES -403G/A and -28C/G polymorphisms confer possible protection against HIV-1 infection, whereas In1.1T/C polymorphism may increase risk of HIV-1 infection, especially in Asians. PMID: 26690919
  25. CCL5 and CXCL11 expression were also induced in response to the activation of the PKC pathway, and gene silencing experiments indicated that their inducible expression was dependent on RIPK4 and IRF6. Moreover, gene reporter assays suggested that RIPK4 induces CCL5 and CXCL11 expression by stimulating the transactivation of their promoters by IRF6. PMID: 27014863
  26. Data suggest that inhibition of CCL5 in adipose microenvironment may represent an approach for the therapy of highly malignant Triple Negative Breast Cancer (TNBC). PMID: 27027351
  27. RNA-binding protein HuR (HuR) expression negatively correlated with chemokine (CC motif) ligand 5 (CCL5) expression and macrophage appearance in a cohort of breast tumors. PMID: 28731284
  28. we have utilized a broad-scaled affinity proteomics approach to identify three proteins (CCL5, HPGDS, and NPSR1) with altered plasma levels in asthmatic children compared to healthy controls, representing the first evaluation of HPGDS and NPSR1 in plasma. PMID: 27145233
  29. Data provide evidence that CCL5 enhances the proliferation and the invasive capacity of human breast cancer cell lines mediated by CCR5 activation. PMID: 27335323
  30. Cancer-FOXP3 serves as a prognostic biomarker and a crucial determinant of immunosuppressive microenvironment via recruiting Treg cells by directly trans-activating CCL5. Therefore, cancer-FOXP3 could be used to select patients with better response to CCL5/CCR5 blockade immunotherapy. PMID: 27991933
  31. data suggest that STAT2 plays a role in the psoriasis pathogenesis by regulating the expression of CXCL11 and CCL5, and thereby attracting IFNgamma-producing immune cells to the skin PMID: 28472186
  32. Mean RANTES concentrations in nasal fluid in patients with perennial allergic rhinitis and nonallergic and allergic chronic rhinosinusitis with nasal polyps patients were significantly higher in comparison to control subjects. PMID: 28587510
  33. All fatty necrotic and osteolytic jawbone (FDOJ) samples showed high expression of RANTES and fibroblast growth factor (FGF)-2. PMID: 28685531
  34. This study showed that RANTES is important in the regulation of vascular dysfunction through modulation of perivascular inflammation. PMID: 26873938
  35. These data indicate that ECFCs, not SPCs, are the major players in MMD pathogenesis and that the chemokine CCL5 mediates the interactions. It can be hypothesized that in MMD patients, defective ECFCs direct aberrant SPC recruitment to critical vascular locations through the action of CCL5. PMID: 28072843
  36. miR-200c represses IL-6, IL-8 and CCL-5 and improves osteogenic differentiation PMID: 27529418
  37. this study shows that melanoma peptides vaccination and intratumoral administration of IFNgamma increases production of CCL5 in patient tumors PMID: 27522581
  38. Findings indicate the importance of chemokine (CC motif) ligand 5 (CCL5) genetic variability and CCL5-CCR5 (CC chemokine receptor 5) axis on the susceptibility to HCV. PMID: 27304910
  39. combined experimentally determined binding affinities (KD) of several orthologs of CCL5 with HNP1 with in silico studies to identify the most likely heterodimeric CCL5-HNP1 complex which was subsequently used as a starting structure to rationally design peptidic inhibitors PMID: 26871718
  40. CCL5 plays a pivotal regulatory role in hepatic fibrosis during nonalcoholic fatty liver disease. PMID: 27639593
  41. Intermolecular interactions of RANTES with its receptor CCR5 have been reported based on NMR spectroscopy measurements. PMID: 28052516
  42. our findings proposed that CCL5 -403G>A polymorphism may be a risk factor for susceptibility TO pulmonary tuberculosis PMID: 27668525
  43. IL-17A could enhance the expression of RANTES, but not IL-16, in cultured primary OFs in cooperation with CD40L. PMID: 27832278
  44. We also found that the activation of H4R caused the release of IL-13 and RANTES on human mast cells.these data demonstrate that the H4R activates divergent signaling pathways to induce cytokine and chemokine production in human mast cells PMID: 27400655
  45. The chemokine RANTES level could become a useful marker of severity of coronary artery disease PMID: 27226191
  46. Findings show the significant upregulated expression of chemokine CCL5 (RANTES) in plasma, compared to CSF and contused brain tissue following severe traumatic brain injury (TBI). PMID: 28340601
  47. There were no associations of CCL5 gene promoter polymorphism with the risk of diabetic microvascular complications (DMI); However, the 59029A polymorphism in CCR5 might affect individual susceptibility for DMI [Meta-Analysis] PMID: 27042273
  48. RANTES Gene Polymorphisms are Associated with HIV-1 Infections. PMID: 27821902
  49. Through the self-production of CCL5, ovarian cancer stem-like cells are induced to differentiate into endothelial cells and participate in tumor angiogenesis. PMID: 27033454
  50. monocytes and lymphocytes cooperate to enhance migration towards CXCR3 chemokines and CCL5 in COPD PMID: 26965295
  51. Results indicated that cisplatin-induced CCL5 secretion derived from the cancer-associated fibroblasts may promote cisplatin resistance, which was mediated by regulation of the STAT3 and PI3K/Akt signal pathways. PMID: 26983899
  52. Authors demonstrate that the inhibition of the CCL5 expression is the cause of the observed effects resulting from RKIP expression. Results underscore the significance of RKIP as important negative regulator of tumor microenvironment. PMID: 26375811
  53. findings demonstrated that CCL5 gene -28C>G polymorphism might be a protective factor for the development of TB PMID: 26620981
  54. The results support the fact that chemokines play important roles in the pathogenesis of HSP. RANTES/CCL5 gene polymorphisms may be related to disease severity and Henoch-Schonlein purpura (HSP) nephritis. PMID: 25839768
  55. This is the first study to note that elevated systemic CCL5 and CXCL6 were associated with moderate/severe lumbar disc degeneration. These chemokines may be systemic biomarkers for the diagnosis and monitoring of disc degeneration. PMID: 26728495
  56. ERalpha decreased CCL5 and IL-6 expression in conditioned media that was collected from Cancer associated fibroblast cell. PMID: 26790618
  57. neutrophil-borne human neutrophil peptide 1 (HNP1, alpha-defensin) and platelet-derived CCL5 form heteromers. PMID: 26659570
  58. High CCL5 expression is associated with neurofibromatosis-1 Glioma. PMID: 26585233
  59. Study provides the evidence that the chemokine receptor CCR5 is highly expressed and associated with poor prognosis in human glioblastoma. Mechanistically, CCL5-CCR5 mediates activation of Akt, and subsequently induces proliferation and invasive responses in U87 and U251 cells. PMID: 26390883
  60. CCL5 signals from infiltrating bone marrow mesenchymal stem cells to HIF2alpha signals within prostate cancer (PCa) cells might play a key role to increase PCa stem cell population and PCa metastasis via altering the androgen receptor signals PMID: 26342197
  61. This meta-analysis showed that RANTES -403G/A and -28C/G were not associated with RA and AD. PMID: 25607251
  62. Data indicate that chemokines CCL5 and CXCL10 serve as the key chemokines to recruit CD8(+) T lymphocytes into esophageal squamous cell carcinoma (ESCC) tissue and may play a role in patient survival. PMID: 26317795
  63. co-cultures of KYSE-410 cells and cancer-associated fibroblasts (CAF) were investigated. Similar to SF, Has2 and Ccl5 were up-regulated and Ccl11 was down-regulated in CAF in co-culture. PMID: 26699196
  64. No relation was seen between RANTES polymorphisms, baseline parasite densities and the time to first re-infection after administration of anti-malaria drugs PMID: 26341782
  65. CCL5 signaling through CCR5 may increase platelet counts during physiological stress. PMID: 26647394
  66. CCL-5-mediated cancer cell migration and invasion are discussed in the context of intertwined feedback loops between breast cancer cells and stromal cells. [review] PMID: 26420723
  67. results suggest the role of RANTES as a potential biomarker of cardiogenic shock and acute heart failure in the hospital phase after myocardial infarction. PMID: 24934326
  68. Results show that low levels of CCL5 were associated with longer duration of viral shedding, suggesting that CCL5 may influence the clearance of norovirus. PMID: 26209395
  69. single nucleotide polymorphism of CCL5-403 G substitution by A may play a role in generalized aggressive periodontitis. PMID: 25119558
  70. a protective role for RANTES -403A allele in male group of patients with Meniere's disease PMID: 24961438
  71. The levels of CCL-5 in men were significantly increased in generalized anxiety disorder and personality disorders PMID: 25560580
  72. This work confirms that OPN, CCL5 and CXCL9 plasma levels are higher in psoriasis patients and provides evidence that their higher levels are not a consequence of obesity. PMID: 25972108
  73. The present work provides insights into the blocking mechanism of HIV-1 by CCL5. PMID: 24965094
  74. CCL5 from ovarian CSLCs activates the NF-kappaB signaling pathway in ovarian non-CSLCs via binding CCR1/3/5, thereby inducing EMT and tumor invasion. PMID: 25788271
  75. The Interaction of Heparin Tetrasaccharides with Chemokine CCL5 Is Modulated by Sulfation Pattern and pH. PMID: 25907556
  76. our findings showed that CCL5/CCR3 signaling promotes metastasis by inducing Th2 polarization of CD4 T cells, with implications for prognosis and immunotherapy of luminal breast cancer. PMID: 26249173
  77. SNPs associated with risk of developing severe enterovirus 71 infection PMID: 26304916
  78. Nuclear beta-catenin localization is related to increased CCL5 production in breast cancer. PMID: 26363360
  79. RANTES -403 G/A polymorphism is a risk factor for asthma, while -28C/G polymorphism is not associated with asthma. PMID: 24963658
  80. Active secretion of CXCL10 and CCL5 from colorectal cancer microenvironments associates with GranzymeB+ CD8+ T-cell infiltration. PMID: 25671296
  81. simple, efficient and high-throughput mass spectrometric immunoassay (MSIA) method for analysis of RANTES proteoforms. RANTES MSIA can detect and analyze RANTES proteoforms and provide an insight into the endogenous protein modifications. PMID: 25549571
  82. Therefore, dengue virus NS5 activates RANTES production by increasing NF-kappaB binding to its binding sites on the RANTES promoter. PMID: 25523420
  83. IL-1b can substantially stimulate the secretion of eotaxin and RANTES in nasal polyp-derived fibroblasts. PMID: 25645983
  84. It is a pro-inflammatory cytokine. PMID: 25421594
  85. Up-regulation of CCL5 is associated with chondrosarcoma. PMID: 25301739
  86. Study indicated that patients with PD have an on-going systemic inflammatory profile where the elevated peripheral production of RANTES may play a role in the neurodegenerative process. PMID: 25587378
  87. Serum CCL5 levels might be utilized as a predictive marker of tumor behavior and disease prognosis in patients with gastric adenocarcinoma. PMID: 25301002
  88. these findings reveal that IKBKE-associated cytokine signaling promotes tumorigenicity of immune-driven TNBC and identify a potential therapeutic strategy using clinically available compounds. PMID: 25365225
  89. Chemokine C-C motif ligand 5 (CCL5) expression was found to be approximately 8-fold higher in old compared to that in young quiescent NHF PMID: 24691968
  90. The CCL5 -403 G>A polymorphism is a risk factor for BC. PMID: 25171476
  91. tumor-associated dendritic cell-derived CCL5 increases human colon cancer cell migration, invasion and epithelial-to-mesenchymal transition in a MALAT-1dependent manner PMID: 25546229
  92. Association between CCL5 polymorphisms and asthma risk: an updated meta-analysis PMID: 25898691
  93. ER stress induced endogenous expression of CCL5 via elevating U-STAT3 expression; however, ER stress inhibited CCL5 secretion, which in turn, decreased the transmigration of breast cancer MCF-7 cells. PMID: 25231320
  94. RANTES concentrations in the serum is primarily dependent upon 1,5-AG plasma levels. PMID: 25373852
  95. a distinct activation of the IFN-stimulated gene signature with a substantial increase in the release of proinflammatory cytokines (IL-6) and chemokines (CXCL10 and CCL5). PMID: 25769924
  96. The most potent inhibition of RANTES, eotaxin and eotaxin3 as well as MMP9 activity. PMID: 25323950
  97. Results show that the G allele of RANTES -28C/G polymorphism was associated with asthma risk in Asian children but not in Caucasian children. PMID: 25004906
  98. There is an increased levels of C-C chemokine RANTES in asbestos exposed workers and in malignant mesothelioma patients from an hyperendemic area. PMID: 25162674
  99. There was an increase in SDF-1 and CCL-5 levels in women and men with different severities of depression, versus the healthy control group PMID: 25150002
  100. High levels of CCL5 expression in mouse and human cell lines might accelerate diffuse large Bcell lymphoma formation in diabetes mellitus. PMID: 24938715

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Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.
Database Links

HGNC: 10632

OMIM: 187011

KEGG: hsa:6352

STRING: 9606.ENSP00000293272

UniGene: Hs.514821

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