Recombinant Human Granulocyte colony-stimulating factor protein(CSF3),partial (Active)

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Code CSB-AP002071HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
Target Names CSF3
Uniprot No. P09919
Research Area Immunology
Alternative Names C17orf33; Colony stimulating factor 3 (granulocyte); Colony stimulating factor 3; CSF 3; CSF beta; CSF3; CSF3_HUMAN; CSF3OS; Csfg; Filgrastim; G-CSF; GCSA; GCSF; Granulocyte colony stimulating factor; Granulocyte colony-stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin
Species Homo sapiens (Human)
Source E.coli
Expression Region 31-204aa
Complete Sequence TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Mol. Weight 18.7 kDa
Protein Length Partial of Isoform Short
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Gene References into Functions
  1. G-CSF and IL-6 concentrations may be predictive of the onset of neonatal Leukemoid reaction. PMID: 29459579
  2. we described the construction and characterization of three G-CSF dimeric proteins generated using different linker peptides. GCSF-Lalpha had the best performance in terms of purity and in vitro activity PMID: 28721592
  3. this study shows that cortisol inhibits CSF3 via DNA methylation and inhibits invasion in first-trimester trophoblast cells PMID: 28846166
  4. We propose that in aggressive pancreatic ductal adenocarcinoma , elevated G-CSF contributes to tumor progression through promoting increases in infiltration of neutrophil-like cells with high immunosuppressive activity. Such a mechanism provides an avenue for a neoadjuvant therapeutic approach for this devastating disease PMID: 28775207
  5. conclusion, there was an elevated lipolysis condition within the FF of Polycystic Ovary Syndrome (PCOS) with metabolic syndrome (MS) patients and the TNF-alpha and G-CSF levels in FF were associated with top-quality embryo percentage. PMID: 28082237
  6. a set of IL-17A-regulated genes in keratinocytes, which recapitulate typical psoriasis genes exemplified by DEFB4A, S100A7, IL19 and CSF3, based on the differences in the expression profiles of cells stimulated with six cytokines versus cells stimulated with only five cytokines lacking IL-17A. PMID: 26944069
  7. The results showed that the addition of recombinant IL-1 markedly increased G-CSF expression in fibroblasts; however, IL-1 receptor antagonist only partially abrogated KCM-stimulated G-CSF expression, indicating the role of additional keratinocyte-releasable factors PMID: 27340768
  8. Data reviewed establish that any damage to brain tissue tends to cause an increase in G-CSF and/or GM-CSF (G(M)-CSF) synthesized by the brain. Glioblastoma cells themselves also synthesize G(M)-CSF. G(M)-CSF synthesized by brain due to damage by a growing tumor and by the tumor itself stimulates bone marrow to shift hematopoiesis toward granulocytic lineages away from lymphocytic lineages. PMID: 28459367
  9. CD146/MCAM is the functional galectin-3-binding ligand on endothelial cell surfaces responsible for galectin-3-induced secretion of metastasis-promoting cytokines. PMID: 28364041
  10. These results suggest that G-CSF could decrease the Th1/Th2 ratio in the context of immune thrombocytopenic purpura, and elucidate the direct and indirect immunomodulatory mechanisms underlying G-CSF functions in Th1/Th2 cells. PMID: 27815970
  11. GCSF impairs CD8(+) T cell functionality by interfering with central activation elements. PMID: 26990855
  12. High expression of G-CSF is associated with Tongue squamous cell carcinoma. PMID: 27316348
  13. this study shows that haplotypes consisting of single nucleotide polymorphisms harboring PSMD3, CSF3 and MED24 genes are associated with asthma in Slovenian patients PMID: 27163155
  14. Cancer cells can produce IL-18, which is involved in the process of angiogenesis, stimulates invasion and metastasis. Decrease in SIPA for the production of IL-6 and GCSF by peripheral blood cells could serve as an indicator of malignant progression in invasive ductal breast carcinoma. PMID: 27021370
  15. G-CSF showed favorable effects only on the migration of HUVECs, and no direct influence was found on Osteoblasts. PMID: 27006951
  16. tumor G-CSF expression is an indicator of an extremely poor prognosis in cervical cancer patients that are treated with chemotherapy. PMID: 26666576
  17. G-CSF stimulates beta1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways. PMID: 26992288
  18. these data suggest that G-CSF may contribute to tumor growth and decrease the antitumor effect of radiotherapy, possibly by promoting vascularization in cancer lesions. PMID: 25976379
  19. in vitro chemotaxis assays and an in vivo transplantation model for chemoattraction confirmed that UCX((R)) are chemotactic to CD34(-)/CD45(-) BM-MSCs via a cell-specific mobilization mechanism mediated by G-CSF. PMID: 24480602
  20. Case Report: undifferentiated colon carcinoma producing G-CSF. PMID: 25400792
  21. increases MMP-2 activity and VEGF secretion in trophoblasts through activation of PI3K/Akt and Erk signaling pathways PMID: 25249155
  22. Elevated IL-8 and G-CSF may be involved in the pathophysiology of narcolepsy. PMID: 24994458
  23. GM-CSF, through its stimulatory function on macrophages, may promote aneurysm progression. PMID: 25389911
  24. G-CSF constrains cancer to grow and progress by, respectively, supporting the survival of sympathetic nerve fibers in 6-hydroxydopamine-sympathectomized mice. PMID: 24975135
  25. Transgenic poultry with a gene of human granulocyte colony-stimulating factor (gcsf) was developed by arti- ficial insemination with the transfected sperm PMID: 25510103
  26. Data suggest that long-term protein secondary structural stability/unfolding of GCSF can be modeled from short-term physicochemical phenomena assessed via spectroscopic measurements. PMID: 24421157
  27. SCF+G-CSF treatment in chronic stroke remodels neural circuits in the aged brain PMID: 23750212
  28. The results demonstrated that 3DHSA-G-CSF has the ability to increase the peripheral white blood cell (WBC) counts of neutropenia model mice, and the half-life of 3DHSA-G-CSF is longer than that of native G-CSF. PMID: 24151579
  29. HNF1A gene was associated with C-reactive protein, and the region including PSMD3 and CSF3 genes was associated with white blood cell count. PMID: 22788528
  30. Administration of G-CSF in a dosage regimen commonly used for bone marrow donors is well tolerated and safe, and provides a signal of positive change in a task of cognitive performance in 8 patients with mild to moderate stage Alzheimer's disease. PMID: 22751169
  31. activation of the RAS/MEK/ERK pathway regulates G-CSF expression through the Ets transcription factor PMID: 23530240
  32. These data suggest that GCSF, which is raised in patient serum, may play an important role in exacerbating disease in ANCA vasculitis. PMID: 23087180
  33. CEACAM1 inhibits both G-CSF production by myeloid cells and G-CSF-stimulated tumor angiogenesis PMID: 23319418
  34. G-CSF level in sera of patients with advanced stages of breast cancer was elevated compared to early stages. PMID: 23244154
  35. G-CSF and VEGF levels in sera might be associated with an early phase of brain protection after birth in severe asphyxia treated with head cooling. PMID: 22944463
  36. Granulocyte-colony stimulating factor contributes to glioma progression that may be linked to glioma genesis and recurrence. PMID: 22313638
  37. Plasma G-CSF is elevated after injury and is greater in patients with shock. The rise in G-CSF is associated with prolonged mobilization of hematopoietic progenitor cells. PMID: 23063381
  38. Treatment of 6-week-old bone marrow stromal cells with GCSF significantly improves their proliferation activity and growth factor production and recovers therapeutic effects in the injured brain. PMID: 21981141
  39. Alteration of Dickkopf-1 and receptor activator of nuclear factor-kappaB ligand during PBSC mobilization in healthy donors by G-CSF. PMID: 22120987
  40. These findings suggest that IL-1beta, IL-1Ra, and granulocyte colony-stimulating factor are functional markers of EV71-related cardiac dysfunction. PMID: 22829643
  41. G-CSF administration at 10 mug/kg/day is safe for patients with worsening symptoms of compression myelopathy and may be effective for their neurological improvement. PMID: 21935680
  42. Non-small cell lung cancer specimens with G-CSF expression exhibited poor differentiation, remarkable atypia, prominent necrosis and infiltration of tumor mass by neutrophils or emperipolesis. PMID: 22336152
  43. Decreased soluble TGF-beta1, Tie-2, and angiopoietins serum levels in bone marrow after treating healthy donors with granulocyte colony-stimulating factor. PMID: 22465760
  44. Elevated plasma GCSF concentration was positively correlated with the severity of ischemic stroke PMID: 22440005
  45. Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signaling. PMID: 22528488
  46. G-CSF can ameliorate cardiac diastolic dysfunction and morphological damage, especially fibrosis of the myocardium, in Otsuka Long-Evans Tokushima fatty rats with diabetic cardiomyopathy. PMID: 21999467
  47. Human recombinant G-CSF enhances angiogenesis following indirect bypass surgery, a combined therapy that is a safe and easy method of treatment. PMID: 21273924
  48. Case Report: diagnosis of G-CSF-producing ascending colon cancer. PMID: 22443081
  49. Prdx4 inhibits G-CSF-induced signalling and proliferation in myeloid progenitors. PMID: 22045733
  50. Data indicate that serum G-CSF levels were lower in JAK2 V617F-positive vs. negative erythrocytosis. PMID: 21645282
  51. G-CSF mobilization not only influences the distribution and expression levels of T cell receptors (TCR) of gamma delta+ T cells (TRGV and TRDV) repertoire, but also changes the clonality of gamma delta+ T cells. PMID: 22171570
  52. A low dose but long duration of GCSF may offer its postulated neuroprotective advantages, without side effects, in improving the functional outcome of toddlers with cerebral palsy when they are given autologous umbilical cord blood transfusions. PMID: 21335665
  53. synergizes with Flt3 ligand in bone marrow stem cell mobilization PMID: 21539498
  54. Leukocyte integrin activation mediates transient neutropenia after G-CSF administration. PMID: 21844566
  55. G-CSF-induced MMP-2 upregulation and invasive phenotype is mediated by Rac1. PMID: 21524920
  56. The beneficial effect of delivering recombinant human G-CSF via an osmotic pump may improve the motor function and enhance neurogenesis in the subventricular zone of following traumatic brain injury. PMID: 20810026
  57. potential biomarker to document the local pre-conception environment for assisted fertilization PMID: 21334074
  58. A systemic dose of human clinical-grade GCSF rapidly induces the motility and entry of neutrophils into blood vessels within the tibial bone marrow of mice. PMID: 21224471
  59. G-CSF restores RARalpha recruitment to target gene promoters through the activation of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway and the subsequent derepression of chromatin. PMID: 21262770
  60. [REVIEW]recent advances in our understanding of the mechanisms by which granulocyte colony-stimulating factor (G-CSF), the prototypical mobilizing agent, induces HSPC mobilization PMID: 21079612
  61. High serum granulocyte Colony-Stimulating Factor is associated with non small cell lung cancer. PMID: 21039040
  62. Suppressed G-CSF production in the presence of IFN-alpha may contribute to IFN-alpha-induced neutropenia. However, a TLR7/8 agonist elicits G-CSF secretion even in the presence of IFN-alpha. PMID: 21092001
  63. G-CSF-mediated hematopoietic stem/progenitor cells (HSPC) mobilization occurs in part through the HGF/c-Met axis in HSPC and myeloid cells, eliciting increased production of matrix-degrading enzymes and subsequently facilitating egress of HSPC. PMID: 20021260
  64. Granulocyte-colony stimulating factor drives the in vitro differentiation of human dendritic cells that induce anergy in naive T cells. PMID: 20957751
  65. G-CSF (granulocyte colony-stimulating factor) expression in tumour cells and the surrounding stroma is not an adverse prognostic factor in ovarian carcinomas PMID: 20012635
  66. Case Report: Report treatment of G-CSF producing lung adenocarcinoma. PMID: 20680910
  67. The transgenic Fn14/TWEAK receptor pathway in a mouse model is adversely involved in inflammatory and ischemic brain disease associated with strong increase of the endogenous neuroprotective G-CSF and the G-CSF receptor system. PMID: 20557950
  68. Recombinant human G-CSF causes type 17 differentiation. Amplification of IL-17 production by G-CSF occurs in both CD4 & CD8 conventional T cells & is dependent on & downstream of G-CSF-induced IL-21 signaling. This causes sclerodermatous GVHD. PMID: 20435882
  69. sequencing of filgrastim by LC-MS/MS method: detection of biotinylation, oxidation, and amidination of specific residues PMID: 19526310
  70. Serum levels of ECP, IL-5, -6, -8 and -10, G-CSF, MCP-1, IL-1 ra and IP-10 were significantly elevated in acute compared with stable childhhood asthma. PMID: 20523065
  71. The significant contribution of PSMD3-CSF3 and PLCB4 loci to the regulation of neutrophil count, is demonstrated. PMID: 20172861
  72. Case Report: Aggressive recurrence of gastric cancer as granulocyte-colony-stimulating factor-producing liver/peritoneal neoplasms. PMID: 20179985
  73. a possible role of G-CSF in malignant transformation, particularly in patients with neutropenia harboring mutations in the gene encoding the G-CSF receptor. PMID: 20237318
  74. Capillary electrophoresis methods showed better separation capacity for the separation of poly(ethylene glycol)-modified GCSFs. PMID: 20361316
  75. Serum G-CSF levels in 70.4% (38 of 54 cases) of the chronic aplastic anemiapatients were increased (272.76 +/- 58.39ng/L) PMID: 15622763
  76. Effect of in vivo infusion of granulocyte colony-stimulating factor on immune function. PMID: 11795665
  77. Induces autocrine production of epithelial cell-derived neutrophil attractant-78 in neutrophils. PMID: 11861308
  78. effect of recombinant human GCSF on dendritic cell populations in mouse spleen PMID: 11877288
  79. G-CSF can induce in vitro and in vivo differentiation of M2 AML t(8;21) cells. PMID: 11920209
  80. Stabilizing the native state of recombinant GCSF under physiological conditions using thermodynamic stabilizers, especially high-affinity ligands binding to the native state, inhibits aggregation occurring under structurally nonperturbing conditions. PMID: 12009905
  81. G-CSF increases neutrophil function in patients with severe sepsis and septic shock PMID: 12027409
  82. expression in airway epithelial cells by interleukin-17 PMID: 12034575
  83. Review. The synergy of granulocyte colony-stimulating factor with stem cell factor results in important biologic responses such as the down-regulation of p27kip1 and the independent phosphorylation of STAT3 on tyrosine and serine residues. PMID: 12152985
  84. inhibits spontaneous cytochrome c release and mitochondria-dependent apoptosis of myelodysplastic syndrome hematopoietic progenitors. PMID: 12393561
  85. results demonstrate that G-CSF is produced in the human follicle shortly before the ovulatory phase and may play an important role in the mechanism of ovulation PMID: 12456601
  86. granulocyte colony-stimulating factor decreased platelet-activating factor acetylhydrolase secretion by decidual macrophages PMID: 12548211
  87. G-CSF-mediated antiapoptosis is mediated through protein synthesis-dependent mechanisms and involves the Janus kinase-STAT pathway. PMID: 12568301
  88. In the presence of ATRA, G-CSF enhanced superoxide release stimulated by the chemotactic peptide, enhanced survival during differentiation, regulated chemotactic peptide receptors CD33 and CD10 but not of CD11b and CD11c. PMID: 12627849
  89. Endogenous G-CSF levels in chemotherapy-induced neutropenia are significantly higher than non-chemotherapy related neutropenia and controls. PMID: 12636092
  90. The induction of GATA-3, the master transcription factor for a Th2 immune response, can be demonstrated in T cells upon G-CSF treatment in vivo accompanied by an increase of spontaneous IL-4 secretion. G-CSF is a strong immune regulator of T cells. PMID: 12676791
  91. tertiary structural perturbations in G-CSF suggest the presence of an intermediate state and possible relation to ligand binding and endocytic trafficking PMID: 12717025
  92. Conformation change of an epitope of the granulocyte-colony stimulating factor after binding to receptors. PMID: 12946100
  93. G-CSF expression in some bladder cancers appears to be an early event during malignant transformation that increases beta1-integrin expression and adhesion and thereby may promote tissue invasion. PMID: 14751388
  94. significant associations of endogenous G-CSF levels with inflammatory mediators early in the development of severe lung injury suggest an endogenous anti-inflammatory role of G-CSF in vivo PMID: 14769149
  95. The significantly higher level of G-CSF in follicular fluid (FF) than in serum and the expression of G-CSF and its receptor in FF by granulosa cells suggest an important role for this growth factor in ovarian function. PMID: 15019810
  96. G-CSF appears to be a suppressor of antitumor immunity PMID: 15214947
  97. G-CSF is associated with the expression of proliferation vascularization in memingioma. PMID: 15218949
  98. The G-CFS may provide clinically useful information, particularly regarding prognosis and response to treatment. PMID: 15576295
  99. G-CSF, a promoter of tolerogenic dendritic cells, may be evaluated for the treatment of human type 1 diabetes PMID: 15616013
  100. one of the major roles of Stat3 in the G-CSF signaling pathway is to augment the function of C/EBPalpha, which is essential for myeloid differentiation PMID: 15664994

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Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

HGNC: 2438

OMIM: 138970

KEGG: hsa:1440

STRING: 9606.ENSP00000225474

UniGene: Hs.2233

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