Recombinant Human Thymic stromal lymphopoietin protein(TSLP) (Active)

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Code CSB-AP002121HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of >3.3x106 IU/mg.
Target Names TSLP
Uniprot No. Q969D9
Research Area Immunology
Alternative Names Thymic stromal lymphopoietin; Thymic stromal lymphopoietin protein TSLP; Tslp; TSLP protein; TSLP_HUMAN
Species Homo sapiens (Human)
Source E.coli
Expression Region 29-159aa
Complete Sequence M+YDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ
Mol. Weight 15.1 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150 mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Isoform 1
Gene References into Functions
  1. TSLP receptor allosterically activates TSLP to potentiate the recruitment of the shared interleukin 7 receptor alpha-chain (IL-7Ralpha) by leveraging the flexibility, conformational heterogeneity and electrostatics of the cytokine. PMID: 28368013
  2. Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells. PMID: 30011850
  3. presence of epithelium-derived TSLP in the eustachian tubes plays important role in the onset of eosinophilic otitis media PMID: 29272985
  4. a new mechanism of T cell stimulation in human skin was identified, demonstrating the direct induction of T cell migration by TSLP in the absence of DCs. PMID: 28377574
  5. the secretion of TSLP by keratinocytes is induced by the HIV-1 viral synapse in a miR-375 controlled manner PMID: 28443609
  6. production increased at skin sites of ovalbumin sensitization PMID: 27992078
  7. Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase in both thymic stromal lymphopoietin and IL-33 levels. PMID: 26948075
  8. short and long thymic stromal lymphopoietin isoforms have roles in house dust mite-induced asthmatic airway epithelial barrier disruption PMID: 27996052
  9. DeltaNp73 was abundantly expressed in the atopic dermatitis epidermis and increased the release of TSLP via NF-kappaB activation. PMID: 28655470
  10. this study shows that long form of thymic stromal lymphopoietin of keratinocytes is induced by protein allergens PMID: 28720058
  11. we found that activation of the protease-activated receptor 1 (PAR1) induced secretion of TSLP by the corneal stromal cells... we proposed that TSLP might function as the link between increased protease activity and inflammatory responses or itch sensation in the corneas PMID: 28631887
  12. TSLP-HOTAIR axis also plays a protective role in low-density lipoprotein (ox-LDL)-induced Endothelial cells' injury. PMID: 28615347
  13. The aim of this study was to determine the influence of human rhinovirus on the genes involved in airway remodeling and to examine the impact of TSLP and contribution of oxidative stress on airway remodeling in the context of HRV infection. PMID: 28545810
  14. TSLP-induced phosphorylation of l-plastin is increased in atopic dermatitis and increases eosinophil migration. PMID: 27304220
  15. a hit expansion exercise yielded additional active compounds that could be amenable to further optimization, providing an opportunity to dissociate TSLP inhibition from other non-desired activities. This study illustrates the potential of phenotypic drug discovery to complement target based approaches by providing new chemistry and biology leads PMID: 29320511
  16. study found that among patients with atopic dermatitis (AD), common FLG null mutations are associated with earlier AD onset in a dose-dependent manner, whereas TSLP rs1898671 appears unrelated to the timing of AD onset PMID: 28479194
  17. lactobacilli from the omnivorous group and all bifidobacteria significantly down-regulated IL-8. Notably, both genera also lowered the TSLP expression in stimulated Caco-2 cells, regardless of the diet regimen. PMID: 27863334
  18. this review focuses on recent advances on the effects of TSLP on obstetrical and gynecological diseases, including pregnancy failure, endometriosis, and cervical cancer PMID: 27976427
  19. The increase in TSLP and TNF-alpha level observed in IS fluid was found to correlate with disease severity. The increased TSLP production from asthma sputum cells was abrogated by the addition of rIL-37. Regulation of TSLP pathway may be a therapeutic approach for asthma. PMID: 27528425
  20. House dust mite sublingual immunotherapy downregulated Th2-type immune responses mediated by the TSLP-OX40L signaling pathway in patients with persistent moderate to severe allergic rhinitis. PMID: 27012942
  21. The results suggest that IL-1beta may be an early key mediator for the acquisition of an Atopic dermatitis (AD) phenotype through induction of thymic stromal lymphopoietin (TSLP) and alteration of the epidermal homeostasis. PMID: 28191908
  22. House dust mite exposure increases the expression of TSLP through PI3K/Akt signaling pathway in human bronchial epithelial 16HBE cells. PMID: 28400057
  23. Posttranslational modifications control the functional activity of TSLP in humans and overproduction of TSLP may be a key trigger for the amplification of type 2 inflammation in diseases PMID: 27744031
  24. Study provides evidence for the hypothesis that TSLP could reflect the histological severity of lumbar disc degeneration (LDD), and TSLP-siRNA and TSLPR-siRNA could inhibit apoptosis of nucleus pulposus cells. The evident information obtained from the investigation could lead the way for new therapeutic approaches regarding LDD treatment. PMID: 28746197
  25. periostin-mediated TSLP production by keratinocytes directly stimulates cutaneous T-cell lymphoma (CTCL) tumor cell growth in addition to inducing a Th2-dominant tumor environment in CTCL. PMID: 27634769
  26. this study shows that TSLP may be involved in the pathogenesis of asthma and rhinitis; dust mite and mugwort allergy could increase it significantly PMID: 28303765
  27. Serum TSLP levels were significantly increased in systemic sclerosis patients compared to healthy donors, and were associated with a higher frequency of vasculopathy. The proportion of TSLP-positive dermal cells was increased in the skin of SSc patients compared with healthy donors, and was correlated with fibrosis. PMID: 27429171
  28. this study shows that NOD2 is an asthma-related factor that can promote cell proliferation and inflammatory response by mediated expression of TSLP in human airway smooth muscle cells PMID: 27889082
  29. the effects of TSLP on myeloid cells are crucial in reducing the multiple organ failure that is associated with systemic inflammation. PMID: 26934097
  30. interactions between TSLP in follicular dendritic cells and IgA production in tonsils may be an important mechanism contributing to the pathogenesis of Immunoglobulin A (IgA) nephropathy. PMID: 27187742
  31. Study demonstrated that naturally occurring severe infections by the most common respiratory viruses in hospitalized infants induce nasal airway secretion of TSLP, IL-33, and periostin when compared with healthy controls PMID: 28471975
  32. Variations in FLG and TSLP genotype were associated with differences in self-reported skin clearance, TCI usage, and steroid usage. PMID: 27902816
  33. A possible role of TSLP in immune homeostasis in HIV infection:higher plasma TSLP was found in individuals with primary HIV infection compared to both chronic HIV infection and healthy controls. PMID: 27769179
  34. The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40) that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4). Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1) in a human cell line (HEK293) expressing NHLH1 resulted in lower TSLP expression PMID: 27149122
  35. TSLP level was significantly higher in asthmatic than in nonasthmatic children. TSLP level was significantly different between allergic asthmatic and nonallergic nonasthmatic groups. PMID: 26999524
  36. While serum TSLP levels were unaffected by concomitant allergies and atopic comorbidities, serum levels of IL-31, IL-33 and sST2 were affected to a small extent. We found a positive correlation between TSLP, IL-31 and IL-33, and an inverse relationship between IL-33 and sST2 PMID: 27152943
  37. Results showed that TSLP promoted the production of alpha-SMA and collagen I (P<0.001), suggesting that it can accelerate MRC-5 cell fibrosis. PMID: 27385084
  38. TSLP is a key mediator in the pathogenesis of inflammatory bowel diseases and that further studies are needed to evaluate its role. [review] PMID: 27697608
  39. Letter: report decreased salivary gland TSLP expression in primary Sjogren's syndrome. PMID: 27494755
  40. A novel mouse xenograft model to study the role of TSLP-induced CRLF2 signals in normal and malignant human B lymphopoiesis has been developed. PMID: 26611474
  41. Heat stress-induced HSPs can significantly reduce the production and secretion of TSLP from keratinocytes cultured under Th2 environment. PMID: 26419317
  42. TSLP expression in the skin is mediated via RARgamma-RXR pathways. PMID: 26531761
  43. Epidermal thymic stromal lymphopoietin predicts the development of atopic dermatitis during infancy. PMID: 26879860
  44. study shows that specific food antigens can trigger innate immune mediated esophageal TSLP secretion PMID: 26992000
  45. production by monocyte-derived dendritic cells requires the integration of signals from dectin-1, the IL-1 receptor, and endoplasmic reticulum stress response stress signaling pathways PMID: 26573878
  46. The association of TSLP polymorphism rs2289278 with atopic dermatitis (AD) was stronger in children with allergic sensitization than in children without atopy, and TSLP polymorphisms also increased the risk of asthma in children with AD PMID: 26712523
  47. The positive feedback loop between TSLP, IL-33 and their receptors, and Th2 cytokines may facilitate Th2-skewed inflammation in eosinophilic CRSwNP. PMID: 26095319
  48. results show that rs1837253 polymorphism may be directly involved in the regulation of TSLP secretion. PMID: 25515628
  49. Upon TSLP stimulation, the cells with the highest TSLPR expression level showed enhanced proliferation and JAK/STAT-mediated gene regulation in a dose-dependent manner PMID: 26652578
  50. Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent PMID: 25962901
  51. Correlation between TSLP overexpression and gastric cancer metastasis to lymph nodes indicate that TSLP plays a role in lymph node involvement in gastric cancer patients. PMID: 26175262
  52. Japanese women with certain TSLP genotypes had a significantly increased risk of eczema. PMID: 25700684
  53. Data suggest that thymic stromal lymphopoietin (TSLP) and interleukin-16 (IL-16), expressed at early stages of disease, function to recruit malignant T cells to the skin. PMID: 24794807
  54. This study indicated that the elevation of microRNA-19b suppressed TSLP expression and then influenced T cell development in thymomatous Myasthenia gravis PMID: 26531692
  55. TRAIL regulates MID1 and TSLP, inflammation, fibrosis, smooth muscle hypertrophy, and expression of inflammatory effector chemokines and cytokines in experimental eosinophilic esophagitis. PMID: 25981737
  56. TSLP secreted by hepatocytes and keratinocytes of HCV-infected patients with CV is involved in the pathogenesis of vasculitis and may possibly support the therapeutic use of TSLP-targeted monoclonal antibodies. PMID: 25889007
  57. LIGHT controls TSLP to drive pulmonary fibrosis. PMID: 25680454
  58. The short TSLP isoform ameliorates experimental colitis in mice and prevents endotoxin shock. The long TSLP isoform is proinflammatory and is only expressed during inflammation. PMID: 26014813
  59. TSLP downregulates filaggrin expression in human skin keratinocytes PMID: 26026341
  60. Positive association exists between serum TSLP and anti-citrullinated peptide antibodies in patients with rheumatoid arthritis. PMID: 25817699
  61. short protein isoform is expressed in keratinocytes of oral cavity, skin and salivary glands, and exhibits a markedly stronger antibacterial activity compared to longer isoform PMID: 24850429
  62. Results suggest that a high level of TSLP in cancer lesions mediated by hypoxia is an important regulator of the progression of cervical cancer by recruiting and licensing tumor-associated EOS to promote the growth of the cervical cancer cell itself. PMID: 25979231
  63. these results suggest that activation of the TSLP pathway is pivotal in the development of pulmonary pathology and pulmonary hMPV replication. PMID: 25763996
  64. TSLP and IL-33 modulate migration of hematopoietic progenitor cells in patients with allergic asthma. PMID: 25656998
  65. Data indicate that co-culture with multiple myeloma (MM) cells increases expression and secretion of thymic stromal lymphopoietin (TSLP in mesenchymal stem cells (MSCs). PMID: 24410591
  66. Interstitial TSLP expression in breast tumors is associated with the prognosis and efficacy of adjuvant chemotherapy. PMID: 25652868
  67. DCs are primed in human asthma for TSLP-driven induction of both Th2 and Th9 cells. PMID: 24888572
  68. In the absence of TP63, keratinocytes readily presented TSLP and other cytokines by stimuli through Toll-like receptor 3. PMID: 25171086
  69. Data indicate that thymic stromal lymphopoietin (TSLP) expression was induced by IL-32 via activation of caspase-1. PMID: 23190696
  70. Higher TGF-beta with lower CD124 and TSLP, but no difference in PAR-2 expression in bronchial biopsy of bronchial asthma patients in comparison with COPD patients. PMID: 24185116
  71. The data reveals a novel effect of allergen-specific T cells as a positive regulator of TSLP production by epithelial cells. PMID: 24773145
  72. In endometriosis, TSLP is involved in estrogen mediated regulation of the secretion of MCP-1 and IL-8, and the growth of endometrial stromal cells through JNK and NF-kappaB signal pathways. PMID: 24966899
  73. might be potential marker of eosinophilic oesophagitis PMID: 24698291
  74. Thymic stromal lymphopoietin: a central regulator of allergic asthma PMID: 24930783
  75. these results support epistasis between SPINK5 and TSLP, which contributes to childhood asthma. PMID: 24831437
  76. circulating TSLP was not significantly associated with eczema, allergen sensitization, or recurrent wheezing during the first three years of life. PMID: 24397611
  77. The results suggest that TSLP may down-regulate caspase3 expression through activation of the STAT3 pathway, thereby suppressing the apoptosis of decidual gammadelta T cells. PMID: 24028796
  78. TSLP synergized with CD40 ligand to promote dendritic cell activation and pathogenic IL-23 production by primary blood and skin dendritic cells. PMID: 24910175
  79. The three polymorphisms in the TSLP were present in the sample population of San Basilio de Palenque and its distribution is similar to that reported for African populations and African ancestry in America PMID: 24652135
  80. Low expression of PPARgamma and high expression of TSLP in the duodenal mucosa of children with newly diagnosed Celiac disease suggest that they are involved in the pathophysiology of this disease PMID: 25187315
  81. airway epithelial cells TRPV1 activation potently induces allergic cytokine thymic stromal lymphopoietin (TSLP) release PMID: 24931369
  82. It plays an important role as a master switch of allergic inflammation because it promotes Th2-type immune responses by inducing the activation of dendritic cells. PMID: 24599138
  83. TSLP induces MUC5B expression via the ERK1/2 and p38 MAPK signaling pathway in human airway epithelial cells. PMID: 24792379
  84. Increased density of intraepithelial mast cells in patients with exercise-induced bronchoconstriction regulated through epithelially derived thymic stromal lymphopoietin and IL-33. PMID: 24220317
  85. Thymic stromal lymphopoietin is a novel proinflammatory mediator in rheumatoid arthritis that potently activates CD1c+ myeloid dendritic cells to attract and stimulate T cells. PMID: 24782181
  86. demonstrate that plasmacytoid DCs, exposed to TSLP together with TLR ligands, acquire significant potential towards Th2 polarization PMID: 24335833
  87. Variants in the gene encoding the TSLP protein may have differential effects on asthma phenotypes depending on gender, atopy, and the presence of allergic rhinitis. PMID: 24525665
  88. TSLP stimulation can induce cellular senescence during airway remodeling in asthma. Inhibiting the signaling pathways of cellular senescence overcomes TSLP-induced airway remodeling PMID: 24167583
  89. TSLP gene variation maybe a potential therapeutic target for the treatment of chronic atopic dermatitis in children. PMID: 24401911
  90. trophoblast-derived thymic stromal lymphopoietin instructs decidual CD11c(+) dendritic cells with increased costimulatory molecules; MHC class II; and Th2/3-type, but not Th1-type, cytokines. PMID: 24453244
  91. inflammasome activation has an inhibitory role on thymic stromal lymphopoietin mRNA expression and T(H)2 cell fate in the skin. PMID: 23953709
  92. There is evidence of TSLP overexpression in systemic sclerosis and other non-allergic fibrotic conditions, and a demonstration of a profibrotic role that is potentially meditated by specific changes in the local cytokine milieu. PMID: 23413283
  93. TSLP is a master regulator of type 2 immune responses beyond that of allergic inflammatory conditions and suggest a novel role for TSLP in the context of chronic fibrotic lung disease. PMID: 24081992
  94. Letter: serum TSLP was not elevated in chronic spontaneous urticaria. PMID: 23602531
  95. Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis PMID: 23688414
  96. these data suggest that the TSLP-basophil axis contributes to the pathogenesis of eosinophilic esophagitis and could be therapeutically targeted to treat this disease. PMID: 23872715
  97. These results demonstrate an essential role for IL-33- and IL-25-independent group 2 innate lymphoid cell in promoting skin inflammation. PMID: 23363980
  98. This study has demonstrated that TSLP secreted by cervical carcinomas cells is involved in the angiogenesis of cervical cancer in a paracrine manner PMID: 23495958
  99. Thymic stromal lymphopoietin could activate dendritic cells (DCs) to differentiate Th17 inflammation in naive CD4(+) T cells. PMID: 23485611
  100. These studies indicate that IL-13 induces AD and atopic march via a TSLP dependent mechanism. PMID: 22355542

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Subcellular Location Secreted
Tissue Specificity Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.
Database Links

HGNC: 30743

OMIM: 607003

KEGG: hsa:85480

STRING: 9606.ENSP00000339804

UniGene: Hs.389874

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