Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x10⁶ IU/mg.

Target Names

Thpo

Uniprot No.

P40226

Research Area

Cancer

Alternative Names

Thpo; Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

22-195aa

Complete Sequence

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

Mol. Weight

18.7 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

0.2 μm filtered PBS, pH 7.4, with 5% Trehalose ,lyophilized

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

Thrombopoietin is the primary physiological regulator of megakaryopoiesis and platelet production, acting through the c-Mpl receptor to drive progenitor cell proliferation and differentiation. This tag-free recombinant mouse Thpo fragment (residues 22–195) encompasses the receptor-binding domain, and its potency is confirmed by an ED50 below 1.0 ng/ml in a MO7e cell proliferation assay, corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg — providing a reliable standard for megakaryocyte colony-stimulating assays, stem cell expansion studies, and receptor-ligand binding experiments using ELISA or SPR. The E. coli expression system yields a non-glycosylated protein, which is advantageous when researchers need to isolate receptor-binding kinetics from glycosylation-dependent variables, and the partial sequence focuses on the biologically active N-terminal domain responsible for c-Mpl engagement. Purity above 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for in vivo tumor biology models and sensitive cell-based proliferation assays.

Target Background

Function

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Gene References into Functions

Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively... IL-11 promoted Hematopoietic stem cell engraftment in a mouse model of aplastic anemia an effect that was attenuated in cells overexpressing miR-204-5p. PMID: 29217821
this study assessed the physiological source of thrombopoietin, a key cytokine required for maintaining hematopoietic stem cell . PMID: 29622652
TPO and its receptor Mpl are dispensable for platelet survival in adult mice PMID: 27344013
Furthermore, although the contribution of the TPO treated graft to long-term hematological engraftment was reduced, the TPO treated and untreated grafts both contributed significantly to long-term chimerism in vivo. PMID: 25137252
TPO treatment also promoted the peripheral induction of Foxp3(+) Tregs in conjunction with elevated circulating TGF-beta levels. PMID: 25212676
novel findings about aspects of TPO action on stem cells PMID: 25564715
Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
Thrombopoietin/MPL signaling confers growth and survival capacity to CD41-positive cells in a mouse model of Evi1 leukemia. PMID: 25298035
Megakaryocytes regulate cell cycle quiescence of hematopoietic stem cell through the production of thrombopoietin. PMID: 25451253
IEX-1 has a role in activation of Erk and NF-kB pathways, which affects thrombopoietin-promoted NHEJ DNA repair in hematopoietic stem cells PMID: 24184684
Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID: 22284746
Signal transduction pathway of ERK1/2 participates in the activation of thrombopoietin in inflammatory injury of BV2 cells. PMID: 21507308
The implication of Tpo as a mediator of neuronal damage in experimental pneumococcal meningitis is reported. PMID: 21149592
Administration of recombinant human IL11 after supralethal radiation exposure promotes survival in mice: interactive effect with thrombopoietin PMID: 12005542
glycan domain of thrombopoietin acts in trans to enhance secretion of the hormone and other cytokines PMID: 12101178
Thrombopoietin expands hematopoietic stem cells after transplantation PMID: 12163458
p38 mitogen-activated protein kinase (MAPK) was responsible for the TPO-induced Hoxb4 elevation PMID: 12855555
Mpl stimulation by TPO results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID: 14726379
Thrombopoietin induces HOXA9 nuclear transport in immature hematopoietic cells PMID: 15254242
mpl-tpo signaling pathways are negatively modulated by lnk PMID: 15337790
is strongly proapoptotic in the brain, causing death of newly generated neurons through the Ras-extracellular signal-regulated kinase 1/2 pathway PMID: 15642952
TPO(high) and GATA-1(low) alterations are linked in an upstream-downstream relationship along a pathobiologic pathway leading to development of myelofibrosis PMID: 15665119
Development of myelofibrosis and osteosclerosis depends on local TPO levels in Bone marrow cells. PMID: 15927672
Thrombopoietin synthesis is induced by IL-6 in the liver during acute inflammation. PMID: 16022585
results suggest that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in HSCs PMID: 17284614
thrombopoietin has a role in mast cell differentiation [review] PMID: 17468237
Chronic TPO overexpression induces mesangioproliferative glomerulopathy. PMID: 17546634
roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit. PMID: 17925492
Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.( PMID: 18410987
TPO induces HIF-1alpha expression in a manner very similar to that of hypoxia. PMID: 18473128
thrombopoietin potentiates vasculogenesis by enhancing motility and enlivenment of transplanted endothelial progenitor cells via activation of Akt/mTOR/p70S6kinase signaling pathway PMID: 18773906
TPO downmodulates c-Myb expression through induction of miR-150. PMID: 18814950

Hide All

Subcellular Location

Secreted.

Protein Families

EPO/TPO family

Tissue Specificity

Found mainly in the liver, kidney and skeletal muscle.

Database Links

KEGG: mmu:21832

STRING: 10090.ENSMUSP00000075756

UniGene: Mm.3943

Code	CSB-AP003451MO
Abbreviation	Recombinant Mouse Thpo protein, partial (Active)
MSDS	MSDS Datasheet
Size	$354
	Order now
Image
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Thrombopoietin protein (Thpo), partial (Active)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage