Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABCD1 Polyclonal antibody

Uniprot No.

Q94FB9

Target Names

ABCD1

Alternative Names

ABCD1 antibody; ACN2 antibody; CTS antibody; PED3 antibody; PMP2 antibody; PXA1 antibody; At4g39850 antibody; T5J17.20 antibody; ABC transporter D family member 1 antibody; ABC transporter ABCD.1 antibody; AtABCD1 antibody; EC 7.6.2.4 antibody; Peroxisomal ABC transporter 1 antibody; AtPXA1 antibody; Protein ACETATE NON-UTILIZING 2 antibody; Protein COMATOSE antibody; Protein PEROXISOME DEFECTIVE 3 antibody; Ped3p antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ABCD1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Contributes to the transport of fatty acids and their derivatives (acyl CoAs) across the peroxisomal membrane. Provides acetate to the glyoxylate cycle in developing seedlings. Involved in pollen tube elongation, ovule fertilization, and seeds germination after imbibition (controls the switch between the opposing developmental programs of dormancy and germination), probably by promoting beta-oxidation of storage lipids during gluconeogenesis. Required for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. Confers sensitivity to monofluoroacetic acid (FAc), a toxic acetate analog, and to 2,4-dichlorophenoxybutyric acid (2,4-DB) and indole-3-butyric acid (IBA), two precursors of auxin after beta-oxidation.

Gene References into Functions

Peroxisomal ATP-binding cassette transporter COMATOSE is required for the accumulation of benzoylated secondary metabolites in Arabidopsis seeds, including benzoylated glucosinolates and substituted hydroxybenzoylcholines. PMID: 24254312
The Arabidopsis peroxisomal ABC transporter, comatose, complements the Saccharomyces cerevisiae pxa1 pxa2Delta mutant for metabolism of long-chain fatty acids and exhibits fatty acyl-CoA-stimulated ATPase activity. PMID: 20659892
PED3 promotes seed germination by suppressing polygalacturonase inhibiting proteins under the control of ABI5. PMID: 20345608
pxa1 plants accumulate free fatty acids in dark-treated leaves. Extended dark conditions are lethal for pxa1 plants. PMID: 19794119
The role of the transporter COMATOSE in the biosynthesis of Jasmonic acid in A. thaliana leaves is described. [COMATOSE] [CTS] PMID: 15761209
CTS has a role following after-ripening within pathways related to the progression of germination prior to radicle emergence. PMID: 16844736
Beta-oxidation could play a role in the mobilization of lipids in both pollen and female tissues. Expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. PMID: 17468211
Results demonstrate that COMATOSE functions can be separated by mutagenesis and the structural model provides a framework for interpretation of phenotypic data. PMID: 19019987
pxa1 mutants are sucrose-dependent and IBA resistant following germination, probably because of a failure to import beta-oxidation substrates into peroxisomes. PMID: 11706205

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Subcellular Location

Peroxisome membrane; Multi-pass membrane protein. Glyoxysome membrane; Multi-pass membrane protein.

Protein Families

ABC transporter superfamily, ABCD family, Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily

Database Links

KEGG: ath:AT4G39850

STRING: 3702.AT4G39850.3

UniGene: At.22795

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ABCD1 Antibody

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