Recombinant Arabidopsis thaliana ABC transporter D family member 1 (ABCC1), partial

Code CSB-YP856736DOA
MSDS
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Source Yeast
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Code CSB-EP856736DOA
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Source E.coli
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Code CSB-EP856736DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856736DOA
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Source Baculovirus
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Code CSB-MP856736DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ABCC1
Uniprot No.
Alternative Names
ABCD1; ACN2; CTS; PED3; PMP2; PXA1; At4g39850; T5J17.20; ABC transporter D family member 1; ABC transporter ABCD.1; AtABCD1; EC 7.6.2.4; Peroxisomal ABC transporter 1; AtPXA1; Protein ACETATE NON-UTILIZING 2; Protein COMATOSE; Protein PEROXISOME DEFECTIVE 3; Ped3p
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Contributes to the transport of fatty acids and their derivatives (acyl CoAs) across the peroxisomal membrane. Provides acetate to the glyoxylate cycle in developing seedlings. Involved in pollen tube elongation, ovule fertilization, and seeds germination after imbibition (controls the switch between the opposing developmental programs of dormancy and germination), probably by promoting beta-oxidation of storage lipids during gluconeogenesis. Required for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. Confers sensitivity to monofluoroacetic acid (FAc), a toxic acetate analog, and to 2,4-dichlorophenoxybutyric acid (2,4-DB) and indole-3-butyric acid (IBA), two precursors of auxin after beta-oxidation.
Gene References into Functions
  1. Peroxisomal ATP-binding cassette transporter COMATOSE is required for the accumulation of benzoylated secondary metabolites in Arabidopsis seeds, including benzoylated glucosinolates and substituted hydroxybenzoylcholines. PMID: 24254312
  2. The Arabidopsis peroxisomal ABC transporter, comatose, complements the Saccharomyces cerevisiae pxa1 pxa2Delta mutant for metabolism of long-chain fatty acids and exhibits fatty acyl-CoA-stimulated ATPase activity. PMID: 20659892
  3. PED3 promotes seed germination by suppressing polygalacturonase inhibiting proteins under the control of ABI5. PMID: 20345608
  4. pxa1 plants accumulate free fatty acids in dark-treated leaves. Extended dark conditions are lethal for pxa1 plants. PMID: 19794119
  5. The role of the transporter COMATOSE in the biosynthesis of Jasmonic acid in A. thaliana leaves is described. [COMATOSE] [CTS] PMID: 15761209
  6. CTS has a role following after-ripening within pathways related to the progression of germination prior to radicle emergence. PMID: 16844736
  7. Beta-oxidation could play a role in the mobilization of lipids in both pollen and female tissues. Expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. PMID: 17468211
  8. Results demonstrate that COMATOSE functions can be separated by mutagenesis and the structural model provides a framework for interpretation of phenotypic data. PMID: 19019987
  9. pxa1 mutants are sucrose-dependent and IBA resistant following germination, probably because of a failure to import beta-oxidation substrates into peroxisomes. PMID: 11706205

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Subcellular Location
Peroxisome membrane; Multi-pass membrane protein. Glyoxysome membrane; Multi-pass membrane protein.
Protein Families
ABC transporter superfamily, ABCD family, Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily
Database Links

KEGG: ath:AT4G39850

STRING: 3702.AT4G39850.3

UniGene: At.22795

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