Gna12 Antibody

Code CSB-PA009584ZA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Gna12 Polyclonal antibody
Uniprot No.
Target Names
Gna12
Alternative Names
Guanine nucleotide-binding protein subunit alpha-12 (G alpha-12) (G-protein subunit alpha-12), Gna12, Gna-12
Raised in
Rabbit
Species Reactivity
Mus musculus
Immunogen
Recombinant Mus musculus Gna12 protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins. Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. Together with NAPA promotes CDH5 localization to plasma membrane. May play a role in the control of cell migration through the TOR signaling cascade.
Gene References into Functions
  1. Galpha12 is required for the development of kidney cysts induced by Pkd1 mutation in mouse autosomal dominant polycystic kidney disease. PMID: 27505895
  2. Our data reveal a previously unknown role for Galpha12 in regulating efferocytosis and that renal tubular epithelial cells require KIM-1 to mediate this process. PMID: 26697979
  3. Selective inhibition of G12/13 signaling in macrophages can augment atheroprotective B-cell populations and ameliorate atherosclerosis. PMID: 26603156
  4. Gpr56 regulates oligodendrocyte development via interactions with Galpha12/13 and RhoA PMID: 25607772
  5. results suggest that KIM-1 is an endogenous protective mechanism against renal ischemia-reperfusion injury through inhibition of Galpha12. PMID: 25759266
  6. Galpha12 may play an important role in promoting membrane trafficking and exocytosis for basal and thrombin-induced vWF secretion, in a process involving SNAP, Galpha12/13 and Galphaq/11 PMID: 24081657
  7. Data indicate that Pyk2 is a common signaling effector downstream of both G12/13 and integrin alphaIIbbeta3 signaling, which contributes to thromboxane generation. PMID: 23640884
  8. PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12 in fibroblasts and pulmonary fibrosis. PMID: 22609986
  9. Expression analysis reveals that production of 5-HT7 and Galpha12 proteins in the hippocampus undergoes strong regulation with a pronounced transient increase during early postnatal stages. PMID: 22378867
  10. that Galpha(i2)-induced signaling can act as a counterbalance to MuRF1-mediated atrophy, indicating that receptors that act through Galpha(i2) might represent potential targets for preventing skeletal muscle wasting. PMID: 22126963
  11. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
  12. These results suggest that Galpha12 may be a critical signaling molecule in TCR-induced IL-2 production and also relay a signal to suppress Th2 and Th17 cell differentiation. PMID: 20233578
  13. The G alpha(12)-mediated signaling pathway functionally interacts not only with the G alpha(13)- but also with the G alpha(q/11)-mediated signaling systems. PMID: 12077299
  14. Hsp90 binding and acylation of Galpha12 results in localization to lipid rafts PMID: 12117999
  15. intracellular Sphingosine 1-phosphate signaled "inside out" through its cell-surface receptors linked to G12/13-mediated stress fiber formation, important for cell motility PMID: 12963721
  16. Galpha(12) stimulates cell proliferation and neoplastic transformation of NIH3T3 cells by attenuating p38MAPK-associated apoptotic responses, while activating the mitogenic responses through the stimulation of ERK- and JNK-mediated signaling pathways. PMID: 14712227
  17. results from differential proteome analysis report a role for SET in Galpha(12)-mediated signaling pathways and a role for Galpha(12) in the regulation of the leukemia-associated SET-protein expression PMID: 15595726
  18. Data demonstrate distinct roles for 5-HT7 receptor/G12 signaling pathways in neurite outgrowth and retraction PMID: 16120784
  19. identified Galpha12/13-proteins as essential components linking extracellular signals to localized Rho-Dia1 function during directed cell movement PMID: 16251183
  20. Galpha12 stimulates the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway through PDGFRalpha. PMID: 16354679
  21. G12/G13 family G proteins contribute to the formation of the mature marginal zone B (MZB) cell compartment both by controlling MZB cell migration and by regulating MZB cell precursor maturation PMID: 16920934
  22. Galpha(12) specifically regulates NF-kappaB-mediated COX-2 induction by S1P downstream of S1P(1), S1P(3), and S1P(5), in a process mediated by the JNK-dependent ubiquitination and degradation of IkappaBalpha PMID: 17098744
  23. G alpha(12/13) regulate basal p53 levels via mdm4, which constitutes a cell signaling pathway distinct from p53 phosphorylations elicited by genotoxic stress. PMID: 17510313
  24. Data suggest that Galpha12 and Galpha13 transmit a JNK-dependent signal for Nrf2 ubiquitination, whereas Galpha13 regulates Rho-PKC delta-mediated Nrf2 phosphorylation, which is negatively balanced by Galpha12. PMID: 17591699
  25. PDGFRalpha and JAK3 have roles in Galpha(12)-mediated cell proliferation PMID: 18038259
  26. G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension PMID: 18084302
  27. AC7 is a specific downstream effector of the G(12/13) pathway PMID: 18541530
  28. Galpha(12) and Galpha(13) regulate expression of TGFbeta1 gene through an increase in Rho/Rac-dependent AP-1 activity, implying that the G-protein-coupled receptor (GPCR)-Galpha(12) pathway is involved in TGFbeta1-mediated transdifferentiation process PMID: 19151758
  29. Constitutive Gs-mediated, but not G12-mediated, activity of the 5-hydroxytryptamine 5-HT7(a) receptor is modulated by the palmitoylation of its C-terminal domain. PMID: 19715731

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Subcellular Location
Cell membrane; Lipid-anchor. Lateral cell membrane; Lipid-anchor. Cytoplasm.
Protein Families
G-alpha family, G(12) subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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