Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Gna12

Uniprot No.

P27600

Research Area

Signal Transduction

Alternative Names

G alpha-12;G-protein subunit alpha-12

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-379aa

Target Protein Sequence

MSGVVRTLSRCLLPAEAGARERRAGAARDAEREARRRSRDIDALLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNILKGSRVLVDARDKLGIPWQHSENEKHGMFLMAFENKAGLPVEPATFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKVKSVSIKKHFPDFKGDPHRLEDVQRYLVQCFDRKRRNRSKPLFHHFTTAIDTENIRFVFHAVKDTILQENLKDIMLQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

51.5 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The process for producing recombinant Mouse Gna12 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Mouse Gna12 protein (1-379aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Mouse Gna12 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.

Target Background

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins. Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. Together with NAPA promotes CDH5 localization to plasma membrane. May play a role in the control of cell migration through the TOR signaling cascade.

Gene References into Functions

Galpha12 is required for the development of kidney cysts induced by Pkd1 mutation in mouse autosomal dominant polycystic kidney disease. PMID: 27505895
Our data reveal a previously unknown role for Galpha12 in regulating efferocytosis and that renal tubular epithelial cells require KIM-1 to mediate this process. PMID: 26697979
Selective inhibition of G12/13 signaling in macrophages can augment atheroprotective B-cell populations and ameliorate atherosclerosis. PMID: 26603156
Gpr56 regulates oligodendrocyte development via interactions with Galpha12/13 and RhoA PMID: 25607772
results suggest that KIM-1 is an endogenous protective mechanism against renal ischemia-reperfusion injury through inhibition of Galpha12. PMID: 25759266
Galpha12 may play an important role in promoting membrane trafficking and exocytosis for basal and thrombin-induced vWF secretion, in a process involving SNAP, Galpha12/13 and Galphaq/11 PMID: 24081657
Data indicate that Pyk2 is a common signaling effector downstream of both G12/13 and integrin alphaIIbbeta3 signaling, which contributes to thromboxane generation. PMID: 23640884
PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12 in fibroblasts and pulmonary fibrosis. PMID: 22609986
Expression analysis reveals that production of 5-HT7 and Galpha12 proteins in the hippocampus undergoes strong regulation with a pronounced transient increase during early postnatal stages. PMID: 22378867
that Galpha(i2)-induced signaling can act as a counterbalance to MuRF1-mediated atrophy, indicating that receptors that act through Galpha(i2) might represent potential targets for preventing skeletal muscle wasting. PMID: 22126963
Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
These results suggest that Galpha12 may be a critical signaling molecule in TCR-induced IL-2 production and also relay a signal to suppress Th2 and Th17 cell differentiation. PMID: 20233578
The G alpha(12)-mediated signaling pathway functionally interacts not only with the G alpha(13)- but also with the G alpha(q/11)-mediated signaling systems. PMID: 12077299
Hsp90 binding and acylation of Galpha12 results in localization to lipid rafts PMID: 12117999
intracellular Sphingosine 1-phosphate signaled "inside out" through its cell-surface receptors linked to G12/13-mediated stress fiber formation, important for cell motility PMID: 12963721
Galpha(12) stimulates cell proliferation and neoplastic transformation of NIH3T3 cells by attenuating p38MAPK-associated apoptotic responses, while activating the mitogenic responses through the stimulation of ERK- and JNK-mediated signaling pathways. PMID: 14712227
results from differential proteome analysis report a role for SET in Galpha(12)-mediated signaling pathways and a role for Galpha(12) in the regulation of the leukemia-associated SET-protein expression PMID: 15595726
Data demonstrate distinct roles for 5-HT7 receptor/G12 signaling pathways in neurite outgrowth and retraction PMID: 16120784
identified Galpha12/13-proteins as essential components linking extracellular signals to localized Rho-Dia1 function during directed cell movement PMID: 16251183
Galpha12 stimulates the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway through PDGFRalpha. PMID: 16354679
G12/G13 family G proteins contribute to the formation of the mature marginal zone B (MZB) cell compartment both by controlling MZB cell migration and by regulating MZB cell precursor maturation PMID: 16920934
Galpha(12) specifically regulates NF-kappaB-mediated COX-2 induction by S1P downstream of S1P(1), S1P(3), and S1P(5), in a process mediated by the JNK-dependent ubiquitination and degradation of IkappaBalpha PMID: 17098744
G alpha(12/13) regulate basal p53 levels via mdm4, which constitutes a cell signaling pathway distinct from p53 phosphorylations elicited by genotoxic stress. PMID: 17510313
Data suggest that Galpha12 and Galpha13 transmit a JNK-dependent signal for Nrf2 ubiquitination, whereas Galpha13 regulates Rho-PKC delta-mediated Nrf2 phosphorylation, which is negatively balanced by Galpha12. PMID: 17591699
PDGFRalpha and JAK3 have roles in Galpha(12)-mediated cell proliferation PMID: 18038259
G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension PMID: 18084302
AC7 is a specific downstream effector of the G(12/13) pathway PMID: 18541530
Galpha(12) and Galpha(13) regulate expression of TGFbeta1 gene through an increase in Rho/Rac-dependent AP-1 activity, implying that the G-protein-coupled receptor (GPCR)-Galpha(12) pathway is involved in TGFbeta1-mediated transdifferentiation process PMID: 19151758
Constitutive Gs-mediated, but not G12-mediated, activity of the 5-hydroxytryptamine 5-HT7(a) receptor is modulated by the palmitoylation of its C-terminal domain. PMID: 19715731

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Subcellular Location

Cell membrane; Lipid-anchor. Lateral cell membrane; Lipid-anchor. Cytoplasm.

Protein Families

G-alpha family, G(12) subfamily

Database Links

KEGG: mmu:14673

STRING: 10090.ENSMUSP00000000153

UniGene: Mm.370185

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Code	CSB-EP009584MO
Abbreviation	Recombinant Mouse Gna12 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Guanine nucleotide-binding protein subunit alpha-12 (Gna12)

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